目的:翼狀胬肉是一種侵襲性增生,是角膜上皮細(xì)胞過度增殖的結(jié)果。分化治療近年來引起了人們的廣泛關(guān)注。本研究的目的是從翼狀胬肉中鑒定干細(xì)胞,并檢測翼狀胬肉干細(xì)胞的分化策略。分化療法的概念來源于激素、細(xì)胞因子或不同分化劑可以促進(jìn)分化,并且可以不可逆地改變細(xì)胞表型。本文簡要地介紹了幾篇文獻(xiàn)和研究,以證明目前的目標(biāo),即分化治療在治療多種臨床疾病中能夠發(fā)揮關(guān)鍵和有前途的作用。方法:應(yīng)用體外原代培養(yǎng)技術(shù)獲得胬肉干細(xì)胞,應(yīng)用免疫熒光染色法研究培養(yǎng)的翼狀胬肉干細(xì)胞的干細(xì)胞標(biāo)志物和多向分化潛能等干細(xì)胞特性。誘導(dǎo)分化后利用克隆形成實驗和MTT法檢測細(xì)胞活力,檢測和評價分化治療策略在胬肉治療中的應(yīng)用潛能。結(jié)果:翼狀胬肉干細(xì)胞可在體外進(jìn)行持續(xù)的培養(yǎng)和擴增。它們表達(dá)了多能干細(xì)胞標(biāo)記物,可在體外誘導(dǎo)分化為神經(jīng)元細(xì)胞、骨細(xì)胞和脂肪細(xì)胞。經(jīng)誘導(dǎo)試驗處理后,翼狀胬肉干細(xì)胞球表達(dá)干細(xì)胞標(biāo)志物和上皮-間充質(zhì)轉(zhuǎn)化標(biāo)志物。誘導(dǎo)分化后,翼狀胬肉干細(xì)胞的增殖和存活能力受到抑制,提示分化誘導(dǎo)策略在翼狀胬肉治療中有廣闊的應(yīng)用前景。結(jié)論:翼狀胬肉來源的細(xì)胞表達(dá)多種干細(xì)胞標(biāo)志物并且具備多向誘導(dǎo)分化潛能。胬肉干細(xì)胞在體外經(jīng)過誘導(dǎo)培養(yǎng)基處理后,... 

【文章來源】：江蘇大學(xué)江蘇省

【文章頁數(shù)】：95 頁

【學(xué)位級別】：博士

【文章目錄】：
摘要
Abstract
1. Background of study
    1.1. Morphology
    1.2. Histopathological findings in pterygia
    1.3. Epidemiology
    1.4. Molecular mechanisms, proteins and genes affected inPterygium
    1.5. Current approaches in the treatment of Pterygium
    1.6. Differentiation therapy and contemporary research
2. Purpose of the study & Experimental design
    Aim of the experiment
    Methods
3. Materials and methods
    3.1. Preparation of pterygiums
    3.2. Emergence of pterygium stem cells
    3.3. Sphere formation of pterygium stem cells
    3.4. H&E immunohistochemistry staining of pterygium tissues
    3.5. Immuno-phenotype
    3.6. Differentiation capacity
    3.7. Analysis of inducing differentiation characteristics in cells
    3.8. Statistical analysis
4. Results
    4.1. Expression level of stemness in superficial level of humanpterygia
    4.2. Expression of multilineage stem cell markers in pterygia
    4.3. Pterygium stem cells sphere formation and its significance
    4.4. Inducing pterygium stem cell differentiation
    4.5. Viability of pterygium stem cells & Significance of MTT;CFA
5.Discussion
6. Conclusion
7. Future perspectives
8. References
9. Acknowledgement


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期刊論文
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