川夏寧坤湯治療痰濕瘀結型子宮腺肌病的作用機制及臨床研究
發(fā)布時間：2018-08-01 19:06
【摘要】：目的及意義:子宮腺肌病(Adenomyosis,AM)是女性的常見病、多發(fā)病,臨床表現(xiàn)如繼發(fā)性痛經(jīng)進行性加重、經(jīng)期延長、經(jīng)量過多、性交痛甚至不孕等,嚴重影響患者生活質量,甚至危害著患者的健康。西藥及手術治療,均存在一定局限性。中醫(yī)認為AM的病機核心是血瘀,而血瘀往往不單獨致病,而與其他病理產(chǎn)物搏結相兼而病,痰濕即是其中之一,痰濕瘀結也成為導致AM的一種重要病機而備受關注。本研究以化痰消ve為法制定方劑"川夏寧坤湯",從宏觀整體研究其對子宮腺肌病患者癥狀及證候的療效。從微觀分子生物學角度,本研究以子宮腺肌病ICR模型小鼠為研究對象,以Survivin、VEGF、Caveolin-1為研究靶點,觀察模型小鼠經(jīng)"川夏寧坤湯"治療后的三組指標表達的差異,從不同角度探討川夏寧坤湯治療子宮腺肌病(痰濕瘀結型)的作用機制,并驗證其療效。研究方法1.實驗研究本研究以小鼠模型為研究對象,在分子蛋白水平檢測Survivin、VEGF、Caveolin-1含量。實驗分為造模與動物實驗兩大部分,M時間持續(xù)長達6個月。1.1研究方法1.1.1造模:將72只新生2天雌性ICR小鼠隨機分為9組:模型組1、2組,空白組1、2組,中成藥組(散結鎮(zhèn)痛組)、西藥組(米非司酮組)、中藥高劑量組、中藥中劑量組、中藥低劑量組,其中模型組、空白組均為2組,每組8只。除空白組以外,其他各組均以他莫昔芬與花生油、卵磷脂、煉乳制成混合液,給予滴灌處理,連續(xù)4天,喂養(yǎng)至3個月,使用組織病理方法觀察肌層是否出現(xiàn)內膜浸潤,以明確造模是否成功,并以免疫組化法檢測模型組、空白組中Survivin、VEGF表達差異,以輔證造模成功與否。1.1.2動物實驗:空白組和模型組小鼠均使用生理鹽水灌胃。中藥組采取"川夏寧坤湯"顆粒劑(配制成高、中、低劑量)分布灌胃,西藥組、中成藥組分布采用米非司酮、散結鎮(zhèn)痛膠囊配制溶液灌胃。灌胃周期3個月,3個月后處死小鼠,通過免疫組化、RT-PCR方法檢測不同組間Survivin、VEGF、Caveolin-1表達的差異。以探究:子宮腺肌病模型小鼠病灶子宮內CAV-1表達與正常小鼠子宮是否存在差異;不同劑量的川夏寧坤湯、米非司酮、散結鎮(zhèn)痛膠囊灌胃后,不同組間Survivin、VEGF、Caveolin-1在小鼠子宮表達的差異。2.臨床研究以"川夏寧坤湯"作為試驗組,以臨床常用治療子宮腺肌病藥物散結鎮(zhèn)痛膠囊作為對照藥物,將痰濕瘀結型AM患者隨機分組進行觀察,以明確"川夏寧坤湯"顆粒的臨床療效。2.1研究方法2.1.1通過試驗組與對照組的比較分析,對"川夏寧坤湯"顆粒減輕痰濕瘀結型子宮腺肌病痛經(jīng)程度、降低持續(xù)時間、減少月經(jīng)血量療效進行評價。2.1.2通過試驗組與對照組的比較分析,對"川夏寧坤湯"顆粒改善痰濕瘀結型子宮腺肌病患者的中醫(yī)證候積分、血清CA125、子宮體積等方面療效進行評價。結果1.實驗研究1.1造模結果:經(jīng)他莫昔芬滴灌4天,并正常飼養(yǎng)至3個月后,處死空白組1組、模型組1組,經(jīng)病理組織切片觀察,可見異位至肌層腺體細胞增生。1.2實驗結果:1.2.1子宮腺肌病小鼠病灶子宮內,Survivin呈高表達;經(jīng)中藥(高劑量組)治療,其表達降低,但中、低濃度組灌胃后表達無明顯下降。1.2.2子宮腺肌病小鼠病灶子宮內,VEGF呈高表達;且經(jīng)中藥(高、中、低劑量組)灌胃后,表達均降低,且無明顯差異(P0.5)。1.2.3子宮腺肌病小鼠病灶子宮內,Caveolin-1呈高表達。經(jīng)中藥(高、中、低劑量)灌胃后,表達降低,且不同濃度組間存在差異(P0.5)。2.臨床研究2.1經(jīng)3個月治療,試驗組與對照組痛經(jīng)NRS評分較治療前下降(P0.5),試驗組與對照組療后比較,試驗組NRS評分明顯低于對照組(P0.5),表明"川夏寧坤湯"顆粒能明顯改善痛經(jīng)程度,且療效優(yōu)于散結鎮(zhèn)痛膠囊。2.2經(jīng)3個月治療,試驗組與對照組COX量表評分較治療前下降(P0.5),試驗組與對照組療后比較,試驗組痛經(jīng)嚴重程度評分明顯低于對照組(P0.5),表明"川夏寧坤湯"顆粒能明顯改善痛經(jīng)程度,且療效優(yōu)于散結鎮(zhèn)痛膠囊,與NRS評分一致;試驗組與對照組療后比較,二者癥狀持續(xù)總時間積分無明顯差異(P0.5)。表明"川夏寧坤湯"顆粒能有效地改善痛經(jīng)持續(xù)時間及程度,在緩解癥狀程度方面療效優(yōu)于散結鎮(zhèn)痛膠囊。2.3經(jīng)3個月治療,試驗組與對照組中醫(yī)證侯積分較療前均有明顯降低(P0.5),兩組療后積分比較無明顯差異(P0.5)。表明"川夏寧坤湯"與散結鎮(zhèn)痛膠囊在治療子宮腺肌病痰濕瘀結型證候積分方面均有明顯療效,療效相近。2.4經(jīng)3個月治療,試驗組與對照組在治療前后子宮體積大小無明顯變化(P0.5),表明"川夏寧坤湯"顆粒在改善子宮體積大小方面無明顯作用。2.5經(jīng)3個月治療,試驗組與對照組在治療前后血清CA125值無明顯下降,表明"川夏寧坤湯"顆粒對降低子宮腺肌病患者血清CA125值方面無明顯作用。2.6經(jīng)3個月治療,試驗組與對照組治療后月經(jīng)量MBL評分較前均有明顯下降(P0.5)。治療后與對照組比較,試驗組改善月經(jīng)量、降低MBL評分方面療效更顯著。表明"川夏寧坤湯"在改善子宮腺肌病患者月經(jīng)量方面療效優(yōu)于散結鎮(zhèn)痛膠囊。2.7經(jīng)3個月治療,試驗組有效率84.84%,對照組有效率62.96%,試驗組有效率高于對照組。表明"川夏寧坤湯"治療子宮腺肌病總有效率高于散結鎮(zhèn)痛膠囊。結論1.實驗研究1.1新生ICR小鼠滴灌他莫昔芬混合液造成子宮腺肌病小鼠模型方法可行。1.2在子宮腺肌病模型小鼠病灶子宮內,Survivin、VEGF均呈現(xiàn)高表達狀態(tài);經(jīng)中藥"川夏寧坤湯"不同濃度灌胃3個月后,VEGF表達明顯降低,Survivin表達僅在使用高劑量中藥灌胃后有所降低。表明"川夏寧坤湯"可能通過抑制新生血管生成、抑制細胞過度增殖而對子宮腺肌病起到治療作用。1.3實驗證實,在子宮腺肌病模型小鼠病灶子宮內Caveolin-1呈高表達。在中藥"川夏寧坤湯"不同濃度組,Caveolin-1表達有明顯差異。2.臨床研究2.1 "川夏寧坤湯"顆粒具有明顯改善子宮腺肌病患者痛經(jīng)癥狀程度、痛經(jīng)程度、持續(xù)時間的作用,可有效改善月經(jīng)量及中醫(yī)證候。2.2 "川夏寧坤湯"顆粒對子宮腺肌病患者子宮體積大小及血清CA125降低無明顯療效。
[Abstract]:Objective and significance: Adenomyosis (AM) is a common and frequently occurring disease of women. The clinical manifestations of secondary dysmenorrhea, such as secondary dysmenorrhea, prolonged menstrual period, excessive menstruation, sexual intercourse and even infertility, seriously affect the quality of life of the patients and even harm the health of the patients. There are certain limitations in western medicine and surgical treatment. Chinese medicine thinks AM The core of the pathogenesis is blood stasis, and the blood stasis often does not cause disease alone, but it is one of the other pathological products. Phlegm dampness is one of them. The phlegm and blood stasis also becomes an important pathogenesis of AM. This study takes the phlegm elimination ve as the prescription of "Sichuan Xia Ningkun soup", and studies the patients with adenomyosis from the macro overall. From the microscopic molecular biology point of view, this study took the ICR model mice of adenomyosis as the research object, with Survivin, VEGF, Caveolin-1 as the research target, observed the difference of the three groups of expression in the model mice after "Chuan Xia Ning Kun soup" treatment, and discussed the treatment of uterine adenomyosis (phlegm dampness) from different angles. The mechanism of the effect of blood stasis type), and to verify its effect. Study methods 1. experimental study in mice model, the molecular protein level of Survivin, VEGF, Caveolin-1 content. The experiment was divided into two parts of model and animal experiment, M time lasted for 6 months.1.1 research method 1.1.1 model: 72 newborn 2 days female ICR was small Rats were randomly divided into 9 groups: Group 1,2 of model group, group 1,2 of blank group, Chinese patent medicine group (dissection analgesic group), western medicine group (mifepristone group), high dose group of traditional Chinese medicine, middle dose group of traditional Chinese medicine, low dose group of traditional Chinese medicine, in which the model group and blank group were all 2 groups with 8 rats in each group. The other groups were made from tamoxifen with peanut oil, lecithin and condensed milk. The mixture was treated with drip irrigation for 4 days, feeding for 3 months, and using histopathology to observe the infiltration of the intima in the myometrium to determine whether the model was successful, and the model group was detected by immunohistochemistry. The difference of Survivin and VEGF expression in the blank group was supplemented by.1.1.2 animal experiment: blank group and model group. The traditional Chinese medicine group took "Chuan Xia Ning Kun soup" granules (prepared into high, medium, low dose) distribution of stomach, the western medicine group, the Chinese patent medicine group distributed using mifepristone, Sanjie analgesic capsule preparation solution gavage. 3 months after the gastric perfusion, 3 months after the death of mice, through immunohistochemical, RT-PCR method to detect Survivin, VEGF, Ca among different groups The difference between the expression of veolin-1 and the difference in the uterus CAV-1 expression in the uterine adenomyosis model mice and the normal mice uterus, the difference between the different doses of the Chuan Xia Ning Kun soup, mifepristone, the Shan Jie analgesic capsule, the difference between the different groups of Survivin, VEGF, Caveolin-1 in the uterus of the mice,.2. clinical study of "Chuan Xia Ning Kun soup" "As the experimental group, the clinical treatment of AM patients with phlegm dampness and stasis type was randomly divided into two groups to observe the clinical curative effect of" Chuan Xia Ning Kun Decoction "(Chuan Xia Ning Kun Decoction) on the clinical therapeutic effect of" Chuan Xia Ning Kun Decoction "and" Chuan Xia Ning Kun Decoction "to reduce the phlegm and dampness and blood stasis through the comparison and analysis between the experimental group and the control group, 2.1.1. The degree of dysmenorrhea, reducing duration and reducing the curative effect of menstrual blood volume of the patients with adenomyosis,.2.1.2 was evaluated through comparison and analysis between the experimental group and the control group. The curative effect of "Chuan Xia Ning Kun Decoction" on the improvement of TCM syndrome score, serum CA125, and the volume of uterus was evaluated. Results 1. experimental research was carried out. Study 1.1 model results: after 4 days of tamoxifen drip irrigation and normal feeding to 3 months after 3 months, 1 groups were killed in the blank group and 1 groups in the model group. The results of.1.2 test of the ectopic and myometrium gland cell proliferation were observed by pathological tissue section. The expression of Survivin was high in the uterus of 1.2.1 uterine adenomyosis of mice, and the expression decreased after the treatment of Chinese medicine (high dose group). In the low concentration group, the expression of VEGF was highly expressed in the endometriosis of.1.2.2 uterine adenomyosis, and the expression decreased after gavage, and there was no significant difference (P0.5) in the uterine adenomyosis of mice (P0.5) in the uterine adenomyosis of mice and the high expression of Caveolin-1. After 3 months, the NRS score of dysmenorrhea decreased (P0.5) in the experimental group and the control group compared with the control group (P0.5). The NRS score of the experimental group was significantly lower than that of the control group (P0.5), which showed that the "Chuan Xia Ning Kun Tang" granule could obviously improve the degree of dysmenorrhea and the curative effect in the experimental group and the control group. The results showed that the effect of "Chuan Xia Ning Kun Tang" can obviously improve the degree of dysmenorrhea, and the effect of the clinical study of the experimental group and the control group was significantly lower than that of the control group (P0.5). After 3 months of treatment, the score of.2.2 in the experimental group and the control group was lower than that before the treatment (P0.5). Compared with the control group, the score of the severity of dysmenorrhea in the experimental group was significantly lower than that of the control group (P0.5), indicating that the "Chuan Xia Ning Kun soup" granule could obviously improve the degree of dysmenorrhea, and the curative effect was better than that of the scattered analgesic capsule and NRS. The scores of the test group and the control group were compared with the control group, and the total time integral of the symptoms of the two patients had no significant difference (P0.5). The results showed that "Chuan Xia Ning Kun Tang" granule could effectively improve the duration and degree of dysmenorrhea, and the curative effect in alleviating the symptom degree was better than that of the Sanjie analgesic capsule for 3 months. The score of the TCM syndrome in the experimental group and the control group was more than that of the control group. Before treatment (P0.5), there was no significant difference between the two groups after treatment (P0.5). It showed that "Chuan Xia Ning Kun soup" and Sanjie analgesic capsule had obvious curative effect in the treatment of syndrome scores of phlegm dampness and stasis syndrome of adenomyosis. The curative effect was similar to that of.2.4 after 3 months, and the size of uterus in the test group and the control group had no obvious changes before and after the treatment. (P0.5) the results showed that "Chuan Xia Ning Kun soup" granules had no obvious effect on the size of the uterus for 3 months. The serum CA125 value of the test group and the control group did not decrease significantly before and after the treatment. It showed that the "Sichuan Xia Ningkun soup" granule had no significant effect on the CA125 value of the patients with uterine adenomyosis for 3 months, and the experimental group was not treated with.2.6. After treatment, the MBL score of the control group was significantly lower than that before the treatment (P0.5). Compared with the control group, the effect of the experimental group on improving the menstrual volume and reducing the MBL score was more significant. The effect of "Chuan Xia Ning Kun Decoction" in improving the menstrual volume of the patients with uterine adenomyosis was better than that of the Sanjie analgesic capsule.2.7 for 3 months, and the effective rate of the experimental group was 84.84. %, the effective rate of the control group was 62.96%, the effective rate of the experimental group was higher than that of the control group. It showed that the total effective rate of "Chuan Xia Ning Kun soup" in the treatment of adenomyosis was higher than that of the Sanjie analgesic capsule. Conclusion 1. experimental study on the mouse model of adenomyosis caused by the mixed solution of tamoxifen in 1.1 newborn ICR mice was feasible in the uterine adenomyosis model mice. The expression of Survivin and VEGF both showed high expression, and the expression of VEGF was obviously reduced after 3 months of gavage of Chinese traditional Chinese medicine "Chuan Xia Ning Kun soup", and the expression of Survivin was reduced only after the use of high dose of traditional Chinese medicine. It showed that "Chuan Xia Ningkun soup" may be treated by inhibiting the formation of neovascularization and inhibiting the excessive proliferation of cells. The.1.3 test in the uterine adenomyosis model mice showed a high expression of Caveolin-1 in the uterine adenomyosis model mice. The expression of Caveolin-1 in the different concentration groups of "Chuan Xia Ning Kun soup" in the Chinese medicine was significantly different from the.2. clinical study 2.1 "Chuan Xia Ning Kun Tang" granules which obviously improved the degree of dysmenorrhea, the degree of dysmenorrhea, and the duration of the duration of the uterine adenomyosis. 2.2 "Chuanxia Ningkun Decoction" Granule has no obvious effect on reducing uterine volume and serum CA125 in patients with adenomyosis.
【學位授予單位】：北京中醫(yī)藥大學
【學位級別】：博士
【學位授予年份】：2017
【分類號】：R271.9
[Abstract]:Objective and significance: Adenomyosis (AM) is a common and frequently occurring disease of women. The clinical manifestations of secondary dysmenorrhea, such as secondary dysmenorrhea, prolonged menstrual period, excessive menstruation, sexual intercourse and even infertility, seriously affect the quality of life of the patients and even harm the health of the patients. There are certain limitations in western medicine and surgical treatment. Chinese medicine thinks AM The core of the pathogenesis is blood stasis, and the blood stasis often does not cause disease alone, but it is one of the other pathological products. Phlegm dampness is one of them. The phlegm and blood stasis also becomes an important pathogenesis of AM. This study takes the phlegm elimination ve as the prescription of "Sichuan Xia Ningkun soup", and studies the patients with adenomyosis from the macro overall. From the microscopic molecular biology point of view, this study took the ICR model mice of adenomyosis as the research object, with Survivin, VEGF, Caveolin-1 as the research target, observed the difference of the three groups of expression in the model mice after "Chuan Xia Ning Kun soup" treatment, and discussed the treatment of uterine adenomyosis (phlegm dampness) from different angles. The mechanism of the effect of blood stasis type), and to verify its effect. Study methods 1. experimental study in mice model, the molecular protein level of Survivin, VEGF, Caveolin-1 content. The experiment was divided into two parts of model and animal experiment, M time lasted for 6 months.1.1 research method 1.1.1 model: 72 newborn 2 days female ICR was small Rats were randomly divided into 9 groups: Group 1,2 of model group, group 1,2 of blank group, Chinese patent medicine group (dissection analgesic group), western medicine group (mifepristone group), high dose group of traditional Chinese medicine, middle dose group of traditional Chinese medicine, low dose group of traditional Chinese medicine, in which the model group and blank group were all 2 groups with 8 rats in each group. The other groups were made from tamoxifen with peanut oil, lecithin and condensed milk. The mixture was treated with drip irrigation for 4 days, feeding for 3 months, and using histopathology to observe the infiltration of the intima in the myometrium to determine whether the model was successful, and the model group was detected by immunohistochemistry. The difference of Survivin and VEGF expression in the blank group was supplemented by.1.1.2 animal experiment: blank group and model group. The traditional Chinese medicine group took "Chuan Xia Ning Kun soup" granules (prepared into high, medium, low dose) distribution of stomach, the western medicine group, the Chinese patent medicine group distributed using mifepristone, Sanjie analgesic capsule preparation solution gavage. 3 months after the gastric perfusion, 3 months after the death of mice, through immunohistochemical, RT-PCR method to detect Survivin, VEGF, Ca among different groups The difference between the expression of veolin-1 and the difference in the uterus CAV-1 expression in the uterine adenomyosis model mice and the normal mice uterus, the difference between the different doses of the Chuan Xia Ning Kun soup, mifepristone, the Shan Jie analgesic capsule, the difference between the different groups of Survivin, VEGF, Caveolin-1 in the uterus of the mice,.2. clinical study of "Chuan Xia Ning Kun soup" "As the experimental group, the clinical treatment of AM patients with phlegm dampness and stasis type was randomly divided into two groups to observe the clinical curative effect of" Chuan Xia Ning Kun Decoction "(Chuan Xia Ning Kun Decoction) on the clinical therapeutic effect of" Chuan Xia Ning Kun Decoction "and" Chuan Xia Ning Kun Decoction "to reduce the phlegm and dampness and blood stasis through the comparison and analysis between the experimental group and the control group, 2.1.1. The degree of dysmenorrhea, reducing duration and reducing the curative effect of menstrual blood volume of the patients with adenomyosis,.2.1.2 was evaluated through comparison and analysis between the experimental group and the control group. The curative effect of "Chuan Xia Ning Kun Decoction" on the improvement of TCM syndrome score, serum CA125, and the volume of uterus was evaluated. Results 1. experimental research was carried out. Study 1.1 model results: after 4 days of tamoxifen drip irrigation and normal feeding to 3 months after 3 months, 1 groups were killed in the blank group and 1 groups in the model group. The results of.1.2 test of the ectopic and myometrium gland cell proliferation were observed by pathological tissue section. The expression of Survivin was high in the uterus of 1.2.1 uterine adenomyosis of mice, and the expression decreased after the treatment of Chinese medicine (high dose group). In the low concentration group, the expression of VEGF was highly expressed in the endometriosis of.1.2.2 uterine adenomyosis, and the expression decreased after gavage, and there was no significant difference (P0.5) in the uterine adenomyosis of mice (P0.5) in the uterine adenomyosis of mice and the high expression of Caveolin-1. After 3 months, the NRS score of dysmenorrhea decreased (P0.5) in the experimental group and the control group compared with the control group (P0.5). The NRS score of the experimental group was significantly lower than that of the control group (P0.5), which showed that the "Chuan Xia Ning Kun Tang" granule could obviously improve the degree of dysmenorrhea and the curative effect in the experimental group and the control group. The results showed that the effect of "Chuan Xia Ning Kun Tang" can obviously improve the degree of dysmenorrhea, and the effect of the clinical study of the experimental group and the control group was significantly lower than that of the control group (P0.5). After 3 months of treatment, the score of.2.2 in the experimental group and the control group was lower than that before the treatment (P0.5). Compared with the control group, the score of the severity of dysmenorrhea in the experimental group was significantly lower than that of the control group (P0.5), indicating that the "Chuan Xia Ning Kun soup" granule could obviously improve the degree of dysmenorrhea, and the curative effect was better than that of the scattered analgesic capsule and NRS. The scores of the test group and the control group were compared with the control group, and the total time integral of the symptoms of the two patients had no significant difference (P0.5). The results showed that "Chuan Xia Ning Kun Tang" granule could effectively improve the duration and degree of dysmenorrhea, and the curative effect in alleviating the symptom degree was better than that of the Sanjie analgesic capsule for 3 months. The score of the TCM syndrome in the experimental group and the control group was more than that of the control group. Before treatment (P0.5), there was no significant difference between the two groups after treatment (P0.5). It showed that "Chuan Xia Ning Kun soup" and Sanjie analgesic capsule had obvious curative effect in the treatment of syndrome scores of phlegm dampness and stasis syndrome of adenomyosis. The curative effect was similar to that of.2.4 after 3 months, and the size of uterus in the test group and the control group had no obvious changes before and after the treatment. (P0.5) the results showed that "Chuan Xia Ning Kun soup" granules had no obvious effect on the size of the uterus for 3 months. The serum CA125 value of the test group and the control group did not decrease significantly before and after the treatment. It showed that the "Sichuan Xia Ningkun soup" granule had no significant effect on the CA125 value of the patients with uterine adenomyosis for 3 months, and the experimental group was not treated with.2.6. After treatment, the MBL score of the control group was significantly lower than that before the treatment (P0.5). Compared with the control group, the effect of the experimental group on improving the menstrual volume and reducing the MBL score was more significant. The effect of "Chuan Xia Ning Kun Decoction" in improving the menstrual volume of the patients with uterine adenomyosis was better than that of the Sanjie analgesic capsule.2.7 for 3 months, and the effective rate of the experimental group was 84.84. %, the effective rate of the control group was 62.96%, the effective rate of the experimental group was higher than that of the control group. It showed that the total effective rate of "Chuan Xia Ning Kun soup" in the treatment of adenomyosis was higher than that of the Sanjie analgesic capsule. Conclusion 1. experimental study on the mouse model of adenomyosis caused by the mixed solution of tamoxifen in 1.1 newborn ICR mice was feasible in the uterine adenomyosis model mice. The expression of Survivin and VEGF both showed high expression, and the expression of VEGF was obviously reduced after 3 months of gavage of Chinese traditional Chinese medicine "Chuan Xia Ning Kun soup", and the expression of Survivin was reduced only after the use of high dose of traditional Chinese medicine. It showed that "Chuan Xia Ningkun soup" may be treated by inhibiting the formation of neovascularization and inhibiting the excessive proliferation of cells. The.1.3 test in the uterine adenomyosis model mice showed a high expression of Caveolin-1 in the uterine adenomyosis model mice. The expression of Caveolin-1 in the different concentration groups of "Chuan Xia Ning Kun soup" in the Chinese medicine was significantly different from the.2. clinical study 2.1 "Chuan Xia Ning Kun Tang" granules which obviously improved the degree of dysmenorrhea, the degree of dysmenorrhea, and the duration of the duration of the uterine adenomyosis. 2.2 "Chuanxia Ningkun Decoction" Granule has no obvious effect on reducing uterine volume and serum CA125 in patients with adenomyosis.
【學位授予單位】：北京中醫(yī)藥大學
【學位級別】：博士
【學位授予年份】：2017
【分類號】：R271.9
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