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水稻分枝調(diào)控蛋白ABR1互作蛋白的鑒定與功能分析

發(fā)布時(shí)間:2019-01-12 14:11
【摘要】:水稻分蘗是決定植株形態(tài)和產(chǎn)量形成的重要農(nóng)藝性狀之一。水稻有效分蘗一般由著生于基部非伸長(zhǎng)節(jié)間上的分蘗芽伸長(zhǎng)生長(zhǎng)而來(lái),而著生于伸長(zhǎng)節(jié)間上的分蘗由于很難長(zhǎng)出稻穗被認(rèn)為是無(wú)效分蘗。為了避免無(wú)效分蘗對(duì)營(yíng)養(yǎng)物質(zhì)的過(guò)多消耗,探索抑制伸長(zhǎng)節(jié)間上分蘗芽伸長(zhǎng)生長(zhǎng)的機(jī)制,對(duì)于改良水稻植株形態(tài)、提高產(chǎn)量形成潛力具有重要意義。前期的研究中,我們鑒定了一個(gè)分枝異常突變體abr1,由于伸長(zhǎng)節(jié)間上的分蘗芽休眠被打破,從而伸長(zhǎng)生長(zhǎng)為分蘗,最終表現(xiàn)為分蘗數(shù)增多。在本研究中,我們發(fā)現(xiàn)ABR1調(diào)節(jié)上位分蘗芽伸長(zhǎng)獨(dú)立于獨(dú)腳金內(nèi)脂途徑。ABR1編碼一個(gè)胞質(zhì)定位的蛋白,與兩個(gè)同源基因K12A/B分別互作。轉(zhuǎn)基因株系k12ak12b中也觀察到了類似于abr1突變體的表型,表明K12A/B具有調(diào)節(jié)水稻伸長(zhǎng)節(jié)間上分蘗芽伸長(zhǎng)生長(zhǎng)的功能。綜合上述研究結(jié)果,初步明確了 ABR1與K12A/B蛋白相互作用調(diào)節(jié)伸長(zhǎng)節(jié)間上分蘗芽的伸長(zhǎng)生長(zhǎng)。
[Abstract]:Rice tillering is one of the important agronomic traits that determine the formation of plant morphology and yield. Rice effective tillers are generally derived from the elongation of tiller buds on non-elongated internodes at the base, while tillers on elongated nodes are considered ineffective because of the difficulty in producing rice panicles. In order to avoid excessive consumption of nutrients by ineffective tillers, it is of great significance to explore the mechanism of inhibiting the growth of tiller buds at elongation Internode, which is of great significance for improving the morphology of rice plants and increasing the potential of yield formation. In previous studies, we identified a mutant with abnormal branching, abr1, which was broken by dormancy of the tiller bud at the elongation Internode, and thus grew into a tiller, which eventually showed an increase in the number of tillers. In this study, we found that ABR1 regulates the elongation of superior tiller buds independent of the monopodium internal lipid pathway. ABR1 encodes a cytoplasmic localization protein and interacts with two homologous genes, K12A/B, respectively. Phenotypes similar to those of abr1 mutants were also observed in transgenic line k12ak12b, indicating that K12A/B could regulate the growth of tiller buds on the elongation internodes of rice (Oryza sativa L.). Based on the above results, the interaction between ABR1 and K12A/B protein was used to regulate the growth of tiller buds at elongation Internode.
【學(xué)位授予單位】:中國(guó)農(nóng)業(yè)科學(xué)院
【學(xué)位級(jí)別】:博士后
【學(xué)位授予年份】:2017
【分類號(hào)】:S511

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