本文關(guān)鍵詞：基于SSR和AFLP標記的東方百合群體遺傳圖譜構(gòu)建及QTL定位　出處：《中國農(nóng)業(yè)科學院》2016年博士論文　論文類型：學位論文

  更多相關(guān)文章： 染色體 DNA標記 花 連鎖圖譜 基因位點 花被片 數(shù)量性狀基因座(QTL) 斑點

【摘要】：百合屬植物是單子葉植物綱百合科的多年生球根花卉,其在全世界范圍內(nèi)分布約有100種(變種),是植物中基因組最大的類群之一。東方百合(Oriental Hybrid lily)是百合中一個重要的雜種系,不同的百合雜種系群體得到構(gòu)建,如AA(亞洲系百合×亞洲系百合),AL(亞洲系百合×鐵炮百合),OT(東方系百合×喇叭百合)等。在本實驗室構(gòu)建了OO(東方百合×東方百合)作圖群體,利用將SSR和AFLP兩種分子標記法構(gòu)建了百合連鎖群。其中,利用本實驗室開發(fā)的495對SSR引物對群體進行檢測。試驗中最終用于構(gòu)建連鎖圖譜的F1群體為100個單株。利用JoinMap4軟件的CP模型對獲得的分子標記數(shù)據(jù)進行處理分析,構(gòu)建了30個遺傳連鎖群圖譜,其中?Sorbonne’(母本)和?Gaudi’(父本)各為12個,F1代為6個。分析觀賞和農(nóng)藝性狀后,用MapQTL 4.0軟件定位遺傳連鎖圖譜中的QTL。主要的研究結(jié)果總結(jié)如下:1.試驗對940對引物組進行了篩選,篩選出172對合適的引物組合(包括96個個AFLP標記和76個SSR標記引物)用于構(gòu)建圖譜。最終共標記出616個位點,其中AFLP標記出465個位點,SSR標記出151個位點。最終獲得616個多態(tài)性位點,其中AFLP位點465個,SSR位點151個。為了對標記進行分組,對連鎖值(LOD)進行了2到10的梯度設置。在分組后,利用LOD為3.0-5.0構(gòu)建了連鎖圖譜。整個圖譜的長度為2144.2cM.在遺傳圖譜中共顯示189個標記位點,其中AFLP位點142個,SSR位點47個。2.母系為12個連鎖群,全長為851.6cM。父本最小和最大的連鎖群的遺傳距離分別為2.2cM和55.7cM。父本遺傳連鎖圖譜共有52個標記位點,其中AFLP位點42個,SSR位點10個。在12個連鎖群的父本遺傳圖譜中,連鎖群全長為676 cM,標記位51個。結(jié)果表明,全長中AFLP標記位點46個,SSR標記位點5個。在母系圖譜中的最小連鎖群距離為0.1 cM,而最大距離為47.7cM。在F1代的6個連鎖群中發(fā)現(xiàn)有86完全評估位點。結(jié)果還表明,AFLP標記數(shù)得分位點為54位點,SSR標記位點有32個。F1代的連鎖群總長度為606.6 cM。F1代最小和最大的連鎖群的距離分別為0.2 cM和26.8 cM。3.檢測到控制重要百合性狀的8個QTL位點,每一個QTL位點可解釋表型變異的2.4%~89.5%。在LG-F1P2上,LOD LOD的最高值的最高值(35.21)下找到了4個QTL。母系的LG-M10上的兩個標記位點間定位到一個有關(guān)葉數(shù)的QTL,其LOD值為7.08。以E-CGC/M-CGC-4為引物,將花被片長度的QTL定位在F1雜交圖譜的LG-F1P2上�；ū黄瑢挾鹊腝TL與LN相似,定位在母系的LG-M10上。PW2的QTL在父本的LG-F4。在F1、母本和父本圖譜上定位有5個關(guān)于斑點數(shù)量的QTL,1個長度為51cM關(guān)于斑點大小的QTL在母系的LG-M8上,以E-CGC/M-CGC-4為引物在F1的LG-F1P2上定位到株高的QTL,其LOD值為12.54。
[Abstract]:Lilium is Monocotyledoneae Liliaceae perennial bulbous flower, its distribution in the whole world there are about 100 species (varieties), is one of the largest groups in the plant genome. Oriental Lily (Oriental Hybrid lily) is one of the most important hybrid lilies in different hybrids groups are constructed, such as AA (Asian Lilium * Asian lily), AL (Asian Lilium longiflorum x), OT (Oriental Lily Lily * horn). A group of OO (Oriental Lily and Oriental Lily) was constructed in this laboratory. The Lilium chain group was constructed by two molecular markers of SSR and AFLP. Among them, 495 pairs of SSR primers developed in our laboratory were used to detect the population. In the experiment, the F1 population, which was finally used to construct the linkage map, was 100 single plants. Based on the CP model of JoinMap4 software, we processed and analyzed the obtained molecular marker data, and constructed 30 genetic linkage map. Among them, Sorbonne and Gaudi were 12 and F1 6, respectively. After analyzing the ornamental and agronomic traits, the MapQTL 4 software was used to locate the QTL in the genetic linkage map. The main results are summarized as follows: 1.. 940 pairs of primers were screened out, and 172 pairs of suitable primer combinations (including 96 AFLP markers and 76 SSR primers) were screened out for constructing map. At last, 616 loci were labeled, of which 465 loci were labeled by AFLP and 151 loci were marked by SSR. In the end, 616 polymorphic loci were obtained, including 465 AFLP loci and 151 SSR loci. In order to group the tags, a gradient setting of 2 to 10 of the chain value (LOD) is performed. After grouping, LOD was used to construct a linkage map for 3.0-5.0. The length of the entire map is 2144.2cM. in the genetic map showing 189 marker loci, including 142 AFLP loci and 47 SSR loci. The 2. matrilineal group is 12 chain groups with a total length of 851.6cM. The genetic distances of the smallest and largest paternal linkage groups were 2.2cM and 55.7cM, respectively. There are 52 marker loci in the paternal genetic linkage map, including 42 AFLP loci and 10 SSR loci. In the paternal genetic map of 12 chain groups, the total length of the linkage group was 676 cM and the marker position was 51. The results showed that there were 46 AFLP marker loci and 5 SSR marker loci in the whole length. The minimum distance of the linkage group in the maternal linkage map is 0.1 cM, and the maximum distance is 47.7cM. 86 complete evaluation sites were found in 6 F1 generation chain groups. The results also showed that the score site of AFLP markers was 54, and there were 32 SSR markers. The total length of the F1 generation chain group was 606.6 cM. The distance between the smallest and the largest group of F1 generation is 0.2 cM and 26.8 cM, respectively. 3. the 8 QTL loci for controlling the important Lily traits were detected, and each QTL locus could explain the 2.4%~89.5% of the phenotypic variation. On LG-F1P2, 4 QTL are found at the highest value (35.21) of the maximum value of the LOD LOD. The two marker loci on the maternal LG-M10 are located between the QTL of the number of leaves and the LOD value of 7.08. Using E-CGC/M-CGC-4 as primers, the QTL of the length of the perianth was located on the LG-F1P2 of the F1 cross map. The width of the perianth of the QTL is similar to that of LN, and is located on the LG-M10 of the maternal line. The QTL of PW2 is in the father's LG-F4. In F1, maternal and paternal maps, there are 5 QTL spots about the number of spots. The 1 51cM is QTL on the maternal LG-M8, with E-CGC/M-CGC-4 as primer, and QTL on the F1 LG-F1P2, the LOD value is 12.54.
【學位授予單位】：中國農(nóng)業(yè)科學院
【學位級別】：博士
【學位授予年份】：2016
【分類號】：S682.29

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本文編號：1344570