本文關(guān)鍵詞：電針促脂肪代謝的腺苷受體機(jī)制　出處：《南京中醫(yī)藥大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 電針 肥胖 腺苷受體 白色脂肪組織 脂肪代謝 能量消耗

【摘要】：目的:觀察電針對(duì)高脂飲食誘導(dǎo)肥胖(diet induced obesity,DIO)小鼠白色脂肪組織(WAT)中腺苷受體的影響,探討腺苷受體在電針減肥中的作用機(jī)制。方法:將63只3周齡雄性C57BL/6小鼠隨機(jī)分為普食組(21只)和高脂組(42只),分別給予普通飲食和高脂飲食喂養(yǎng)12周后,以體質(zhì)量超過(guò)正常小鼠平均體質(zhì)量的20%作為肥胖模型標(biāo)準(zhǔn),并最終將小鼠隨機(jī)分為正常對(duì)照組(8只)、正常電針組(13只)、模型對(duì)照組(9只)、模型電針組(14只)。兩個(gè)治療組均電針"后三里"和"內(nèi)庭"穴,每周治療6次,共電針4周。測(cè)量小鼠體質(zhì)量和附睪脂肪組織質(zhì)量,計(jì)算小鼠體重變化以及脂肪組織脂體比;用代謝籠監(jiān)測(cè)小鼠熱量釋放、攝食、活動(dòng)量(XT+YT)和總移動(dòng)路程;HE染色評(píng)價(jià)脂肪細(xì)胞的體積;用qPCR技術(shù)檢測(cè)脂肪組織中A1R、A2AR、A2BR、A3R的mRNA表達(dá)以及棕色化相關(guān)基因Tbx1、Tmem26的表達(dá);用Western Blot檢測(cè)脂肪組織中腺苷受體、PRDM16、PGC-1α、UCP1、pTH、pHSL 的蛋白表達(dá)。結(jié)果:實(shí)驗(yàn)一:1.與普食組相比,高脂喂養(yǎng)12周能明顯增加小鼠體質(zhì)量(P0.01),其中,體質(zhì)量超過(guò)普食組20%的小鼠有23只(占54.76%)。2.治療結(jié)束后,與正常對(duì)照組相比,正常電針組小鼠體質(zhì)量和附睪脂肪組織(Epi-WAT)脂體比下降(P0.05),模型對(duì)照組體質(zhì)量依然維持在較高狀態(tài)(P0.05)、體質(zhì)量變化值差異有統(tǒng)計(jì)學(xué)意義(P0.01)、Epi-WAT質(zhì)量和脂體比升高(P0.01,P0.05);與模型對(duì)照組相比,模型電針組體質(zhì)量、Epi-WAT脂體比均降低(P0.05)、體質(zhì)量變化值增大(P0.01)。3.代謝籠:與正常對(duì)照組相比,模型對(duì)照組VO2水平下降(P0.05)、呼吸交換率(Respiratory exchange rate,RER)升高(P0.05)、攝食量升高(P0.05)、活動(dòng)量(XT+YT)和總移動(dòng)路程無(wú)顯著差異(P0.05);與模型對(duì)照組相比,模型電針組H1、H2、V02、VC02水平均升高(P0.05),RER無(wú)統(tǒng)計(jì)學(xué)差異(P0.05),攝食量下降(P0.05),XT+YT、總移動(dòng)路程無(wú)顯著差異(P0.05)。4.HE染色:與正常對(duì)照組相比,正常電針組附睪脂肪細(xì)胞面積減小(P0.05),模型對(duì)照組面積增大(P0.01);與模型對(duì)照組相比,模型電針組面積減小(P0.01)。實(shí)驗(yàn)二:1.A1RmRNA在四組中表達(dá)的差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);與正常對(duì)照組相比,正常電針組A2AR和A2BR蛋白表達(dá)上調(diào)(P0.05,P0.01),模型對(duì)照組A3R mRNA的表達(dá)降低(P0.01)、A2AR蛋白表達(dá)降低(P0.05)、A2BR蛋白表達(dá)差異不明顯(P0.05);與模型對(duì)照組相比,模型電針組A2AR、A2BR mRNA表達(dá)升高(P0.05),A2AR和A2BR蛋白表達(dá)升高(P0.01)。2.與正常對(duì)照組相比,模型對(duì)照組Tbx1、Tmem26 mRNA表達(dá)降低(P0.05),BAT中PRDM16蛋白水平下降(P0.05);與模型對(duì)照組相比,模型電針組Tbx1、Tmem26mRNA水平上調(diào)(P0.05),BAT中PRDM16、UCP1蛋白水平上調(diào)(P0.01),Epi-WAT 中 PRDM16、PGC-1α、UCP1 的蛋白表達(dá)升高(P0.05,P0.05,P0.01)。3.與正常對(duì)照組相比,正常電針組pTH蛋白表達(dá)升高(P0.05),模型對(duì)照組pTH、pHSL蛋白水平下降(P0.01,P0.05);與模型對(duì)照組相比,模型電針組pTH、pHSL蛋白水平均升高(P0.01)。結(jié)論:電針可能通過(guò)激活自主神經(jīng)系統(tǒng),調(diào)節(jié)WAT中A2AR、A2BR的表達(dá),促進(jìn)脂肪代謝和能量消耗,這可能是電針減肥的部分機(jī)制。
[Abstract]:Objective: To observe the effect of Electroacupuncture on adenosine receptor in white adipose tissue (WAT) of diet induced obesity (DIO) mice induced by high-fat diet, and to explore the mechanism of adenosine receptor in reducing weight by electroacupuncture. Methods: 63 3 week old male C57BL/6 mice were randomly divided into normal control group (21 rats) and high-fat group (42 rats), which were fed with normal diet and high-fat diet for 12 weeks, with the body mass of more than 20% of the average body weight of normal mice as obesity model standard, and finally the mice were divided into normal control group (8 rats), normal acupuncture group (13 rats), model group (9 rats), model group (14 rats) electroacupuncture. The two treatment groups were treated with electroacupuncture "after three li" and "inner court", 6 times a week for 4 weeks. Measurement of mice body weight and epididymal adipose tissue mass, calculate the weight changes of mice adipose tissue and body fat ratio; release, using metabolic cages of mice feeding and activity monitoring heat capacity (XT+YT) and the total moving distance; HE staining was used to evaluate the size of fat cells; using qPCR to detect the fat tissue of A1R, A2AR, A2BR, A3R the expression of mRNA and browning related gene Tbx1 and Tmem26 expression; expression of adenosine Blot receptors in the detection of adipose tissue Western, PRDM16, PGC-1, UCP1, pTH, alpha pHSL protein. Results: Experiment 1: 1., compared with the common food group, high fat diet can significantly increase body weight (P0.01) in 12 weeks, and the body weight of the mice is 20% higher than that of the common food group (23) (54.76%). 2. after the end of treatment, compared with the normal control group, normal acupuncture group of mice body weight and epididymal adipose tissue (Epi-WAT) decreased body fat (P0.05), model control group, the body mass is still maintained at a high state (P0.05), the mass variation value difference was statistically significant (P0.01), Epi-WAT quality and ratio of fat. Increased (P0.01, P0.05); compared with the model group, EA group model body mass, fat body were lower than Epi-WAT (P0.05), the changes of body weight increases (P0.01). 3. metabolic cages: compared with normal control group, model control group, VO2 decreased (P0.05), respiratory exchange ratio (Respiratory exchange rate, RER) (P0.05), increased food intake increased (P0.05), activity (XT+YT) and no significant difference between the total mobile distance (P0.05); compared with the model control group model H1, H2, V02 EA group, the level of VC02 increased (P0.05), RER (P0.05), there was no significant difference in food intake decreased (P0.05), XT+YT had no significant difference, the total mobile distance (P0.05). 4.HE staining: compared with the normal control group, the area of epididymal adipocytes in the normal electroacupuncture group decreased (P0.05), and the area in the model control group increased (P0.01). Compared with the model control group, the area of the EA group decreased (P0.01). Experiment two: there was no significant difference in the expression of 1.A1RmRNA in the four groups (P0.05); compared with the normal control group, normal acupuncture group of A2AR and A2BR protein expression (P0.05, P0.01), model control group A3R expression decreased mRNA (P0.01), lower expression of A2AR (P0.05) and A2BR protein expression difference obviously (P0.05); compared with the model group, the increased expression of A2AR, A2BR, mRNA model EA group (P0.05), the expression of A2AR and A2BR protein level (P0.01). 2. compared with normal control group, model control group Tbx1, Tmem26 decreased expression of mRNA (P0.05), PRDM16 protein level in BAT decreased (P0.05); compared with the model control group, model group, Tbx1 EA increased level of Tmem26mRNA (P0.05), the level of PRDM16 and UCP1 protein in BAT (P0.01), increased expression of Epi-WAT in PRDM16 PGC-1, alpha, UCP1 protein level (P0.05, P0.05, P0.01). 3., compared with the normal control group, the expression of pTH protein in the normal electroacupuncture group increased (P0.05), and the pTH and pHSL protein levels in the model control group decreased (P0.01, P0.05). Compared with the model control group, the pTH and pHSL protein levels in the EA group increased (P0.01). Conclusion: EA may regulate the expression of A2AR and A2BR in WAT and promote lipid metabolism and energy consumption by activating the autonomic nervous system, which may be a part of the mechanism of electro acupuncture for weight loss.
【學(xué)位授予單位】：南京中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R245

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