山海螺化學成分與質量控制研究
發(fā)布時間:2017-12-26 20:28
本文關鍵詞:山海螺化學成分與質量控制研究 出處:《北京中醫(yī)藥大學》2017年碩士論文 論文類型:學位論文
【摘要】:本論文包括兩部分實踐部分和實驗部分。論文第一部分為中日友好醫(yī)院藥學部實踐部分。分別是門診中藥房、住院中藥房、中藥庫、中藥制劑室、藥檢室。在這5個部門實踐過程中,學習和了解了各部門的工作流程及注意事項。論文第二部分為是山海螺的化學成分與質量控制研究。主要分為三個部分,一、山海螺藥材的研究進展;二、山海螺的化學成分研究;三、山海螺的質量控制研究。第一部分對山海螺的化學成分、藥理作用、臨床應用等方面進行論述。闡述了山海螺的化學成分主要為三萜皂苷類化合物,此外,還含有糖類、甾醇、生物堿類、黃酮類及揮發(fā)油等化合物。具有抗癌、抗突變、抗氧化、降血糖、降血脂、鎮(zhèn)痛等作用。第二部分為山海螺的化學成分研究,采用HP-20大孔樹脂、Sephadex LH-20柱色譜等色譜分離方法對山海螺50%乙醇洗脫部位進行化合物分離純化,通過1 H-NMR和13 C-NMR等波譜技術和化學方法鑒定所分離純化得到的化合物結構。通過反復上SephadexLH-20柱色譜共分離得到6個化合物,鑒定了 5個化合物,分別是5-羥甲基-2-呋喃甲醛(1)、CodonopilodiynosideA(2)、lobetyol(3)、(+)-isolariciresinol(4)、黨參炔苷(5)。其中化合物(1)、(2)、(3)、(4)均為首次從該植物中分得。第三部分為山海螺的質量控制研究,運用HPLC建立黨參炔苷含量測定的方法學,同時共收集了 13批山海螺藥材進行HPLC測定,測得色譜圖,運用中國藥典委員會"中藥色譜指紋圖譜相似度評價軟件A"進行評價,生成對照圖譜。該方法操作簡便快捷,具有專屬性強、穩(wěn)定性、重復性良好等特點,可用于山海螺藥材的質量控制與評價。
[Abstract]:This paper includes two parts of practice and experiment. The first part of the thesis is the practical part of the pharmacy department of China-Japan Friendship Hospital. They are the pharmacy in the outpatient department, the pharmacy in the hospital, the Chinese medicine library, the Chinese medicine preparation room and the medicine inspection room. During the practice of these 5 departments, we learn and understand the work flow and attention of various departments. The second part of the thesis is to study the chemical constituents and quality control of Codonopsis lanceolata. Mainly divided into three parts. The research progress of medicinal materials, a study on the chemical constituents of Codonopsis lanceolata; two, the research on the quality control of Codonopsis lanceolata; three, Codonopsis lanceolata. The first part of Codonopsis lanceolata chemical composition, pharmacological effects and clinical application were discussed. Describes the three main chemical composition of Codonopsis lanceolata saponins compounds, in addition, also contains carbohydrates, sterols, alkaloids, flavonoids and volatile oil compounds. It has the effect of anticancer, anti - mutation, antioxidation, hypoglycemic, antilipidemia and analgesic effect. The second part is the study on the chemical constituents of Codonopsis lanceolata, using the separation method of HP-20 macroporous resin column chromatography, Sephadex LH-20 chromatography for separation and purification of compounds of 50% ethanol elution fraction of Codonopsis lanceolata, structure of pure compounds obtained by the separation of 1 H-NMR and 13 C-NMR spectra and chemical identification methods. 6 compounds were isolated by repeated SephadexLH-20 column chromatography. 5 compounds were identified, namely, 5- hydroxymethyl -2- furan formaldehyde (1), CodonopilodiynosideA (2), lobetyol (3), (+) -isolariciresinol (4), and Codonopsis glucoside (5). The compounds (1), (2), (3) and (4) were all derived from the plant for the first time. The third part is the research on the quality control of Codonopsis lanceolata, using HPLC to establish a method for determination of Lobetyolin content, and collected a total of 13 batches of medicinal materials of Codonopsis lanceolata HPLC were measured using Chinese chromatogram, Pharmacopoeia Commission "chromatographic fingerprint similarity evaluation software A to evaluate the generated control map. This method is simple and quick, has the characteristics of strong specificity, stability, good repeatability, and can be used for quality control and evaluation of medicinal materials of Codonopsis lanceolata.
【學位授予單位】:北京中醫(yī)藥大學
【學位級別】:碩士
【學位授予年份】:2017
【分類號】:R284.1
【參考文獻】
相關期刊論文 前10條
1 趙鵬飛;;山海螺本草考證與臨床研究進展[J];遼寧中醫(yī)藥大學學報;2014年03期
2 陳慧慧;牛偉霞;李士棟;張淹;趙紅燕;鄭海榮;;HPLC測定不同產地黨參中黨參炔苷的含量[J];食品與藥品;2014年01期
3 閆珍;陳培豐;;中醫(yī)藥治療肺癌研究進展[J];浙江中西醫(yī)結合雜志;2013年10期
4 馮佩佩;李忠祥;原忠;;黨參屬藥用植物化學成分和藥理研究進展[J];沈陽藥科大學學報;2012年04期
5 張峰;高永峰;張繼國;;四葉參多糖對糖尿病大鼠血糖及免疫功能的影響[J];中國實驗方劑學雜志;2012年02期
6 李鐵軍;;輪葉黨參的研究進展[J];中國現代中藥;2011年06期
7 王文永;趙仕丞;劉東新;;山海螺化學成分研究[J];中藥材;2011年04期
8 曹煦;李創(chuàng)軍;楊敬芝;魏佰興;羅永明;張東明;;雷公藤葉化學成分研究[J];中國中藥雜志;2011年08期
9 陳恒沖;馬彬峽;李景輝;;輪葉黨參藥材生藥學研究[J];中國民族民間醫(yī)藥;2010年23期
10 宋龍;張紅梅;周秀佳;;羊乳的本草考證[J];醫(yī)學信息(中旬刊);2010年06期
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