隨著淡水水體富營養(yǎng)化程度的加劇,藍藻水華（Cyanobacterial blooms）在全球范圍的爆發(fā)日趨嚴重,對水生生態(tài),經(jīng)濟,甚至是人類的健康都可能產(chǎn)生不良影響,引發(fā)了嚴重的環(huán)境問題。目前,已經(jīng)有大量關(guān)于藍藻水華控制和藍藻毒素降解的相關(guān)研究。其中溶藻微生物（溶藻細菌）已經(jīng)被作為一種可以抑制藍藻水華和降解藍藻毒素的方法而得到廣泛研究。然而,在溶藻微生物的相關(guān)研究中,除了對一種可產(chǎn)生抗菌素真菌的抑藻能力的研究外,對于溶藻真菌的情況還知之甚少。與此同時,盡管溶藻細菌有一定的抑制藻類生長的能力,但是相比于真菌而言,隨著溶藻細菌自身的繁殖,細胞數(shù)量的增加會大大降低水體的透明度,且溶藻細菌的次生代謝產(chǎn)物多,變異快,不易控制等缺點都會影響到溶藻細菌的實際應(yīng)用。自2007年以來,我國太湖也爆發(fā)了大規(guī)模的藍藻水華,對當(dāng)?shù)氐娜嗣裆?經(jīng)濟及社會的發(fā)展都造成了嚴重的影響。為了能夠更好的控制藍藻水華,減小其造成的危害,本研究分離到兩株白腐真菌Trichaptum abietinum 1302BG和Lopharia spadicea,并對其抑藻能力進行了研究。1.本論文研究了兩株白腐真菌對不同淡水藍藻的抑制... 

【文章來源】：南京大學(xué)江蘇省 211工程院校 985工程院校 教育部直屬院校

【文章頁數(shù)】：131 頁

【學(xué)位級別】：博士

【文章目錄】：
LIST oF ABBREVIATIONS
摘要
ABSTRACT
CHAPTER 1:INTRODUCTION
    1. CYANOBACTERIA
    2. HARMFULALGAL BLOOMS(HABs)
        2.1 Harmful cyanobacteria
        2.2 Harmful cyanobacterial blooms
    3. RESEARCH OBJECTIVES
    REFERENCES
CHAPTER 2:SCREENING FUNGAL STRAINS FROM BAMBOO SOILS AND EVALUATE THEIR ALGAE INHIBITION CAPACITIES AND MECHANISMS
    1. INTRODUCTION
    2. MATERIALS AND METHODS
        2.1. Cyanobacterial strains and cultivation
        2.2. Fungal isolation,identification,culture and maintenance
        2.3. Degradation test of TA-1302 on algae
        2.4. Lipid peroxidation
        2.5. Biomass of mycelial pellicle
        2.6. Algae lysis
        2.7. Scanning electron microscopy (SEM)
        2.8. Transmission electron microscopy (TEM)
        2.9. Statistical analysis
    3. Results
        3.1. Changes in algal cells during incubation
        3.2. Changes in lipid peroxidation
        3.3. The mass of mycelial pellicle
        3.4. Degradation mechanism of the algal cells
        3.5 Identification and phylogenetic analysis of TA-1302
    4. Discussion
    References
CHAPTER 3: A CAPABLE OF TRICHAPTUM ABIETINUM 1302BG ONDEGRADING MICROCYSTIN-LR AND REDUCING BIOMASS IN THEALGAL CULTURE
    1. Introduction
    2. Materials and methods
        2.1. Phytoplankton strains and cultivation
        2.2 Fungal isolation, culture, and maintenance
        2.3 Degradation test of TA-1302 on algae and MC-LR
        2.4 HPLC analysis
        2.5 Antioxidant enzyme activity assays
        2.6 Toxicity studies
        2.7 Statistical analysis
    3. Results
        3.1 The biomass of algal cultures
        3.2 Changes in concentration of MC-LR
        3.3 Effects of MC-LR on antioxidant enzymes
        3.4 The toxicity of degraded algal cuture
    4. Discussion
    References
CHAPTER 4: EVALUATION OF ALGICIDAL ABILITY WITH FUNGI ONDIFFERENT FRESHWATER ALGAE SPECIES
    1. Introduction
    2. Materials and methods
        2.1. Algal strains and cultivation
        2.2. Fungal strains and maintenance
        2.3. Inhibiting tests of different fungi on different algal species
        2.4. Dry weight of mycelial pellicle
        2.5. Statistical analysis
    3. Results
        3.1 Preliminary screening of algicidal fungi
        3.2 Variations in biomass of algicidal fungi during incubation
    4. Discussion
    References
CHAPTER 5: A NEW STRATEGY FOR DEALING WITH THECYANOBACTERIAL BLOOMS
    1. Introduction
    2. Materials and methods
        2.1 Site and sampling
        2.2 Fungal culture and maintenance
        2.3 Growth test
        2.4 Degradation test of TA-1302 and LS-1302 on cyanobacterial bloom algae
        2.5 Statistical analysis
    3. Results
        3.1 Mycelial plaque of TA-1302 andLS-1302
        3.2 Dry weight of TA-1302 andLS-1302
        3.3 Changes in biomass of culture during incubation
    4. Discussion
    References
CHAPTER 6: SUMMARY
    6.1 Conclusions
    6.2 Main innovation
    6.3 Prospects
SUPPLEMENTAL MATERIAL
PRODUCTIONS
ACKNOWLEDGEMENTS
附件


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期刊論文
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