【摘要】：國內(nèi)外對細菌的作用及作用機制研究較多,而對真菌的作用及機制研究較少.為了提高NO,NO_x的脫除效率,本文對脫氮塔生物膜中的反硝化真菌進行了分離、純化及鑒定,通過搖瓶實驗研究了反硝化真菌對NO_3~-的作用特征,最后在脫氮塔中投加了分離的反硝化真菌純菌株擴大培養(yǎng)液,研究了其對NO_x脫除效率的影響,還對脫氮塔內(nèi)真菌的群落結(jié)構(gòu)進行了高通量測序分析.實驗結(jié)果表明:經(jīng)過真菌形態(tài)特征和脫氮(NO_3~-)特性研究及ITS序列測定分析,將3LNB菌株鑒定為鐮刀菌的腐皮鐮刀菌Fusarium solani.高通量測序分析結(jié)果證明了脫氮塔內(nèi)確實存在鐮刀菌屬的腐皮鐮刀菌Fusarium solani.在NO_3~-—N濃度高達10.01g/L的條件下,實驗共10d,細菌反硝化組反硝化率為5.6%,其脫除效率為0.56g/L;真菌3LNB菌株的反硝化率能達到15.1%,其脫除效率為1.51g/L.實驗室實驗證明3LNB菌株對NO_3~-具有較強的反硝化能力.將3LNB純菌株擴大培養(yǎng)液400mL(4%)投加到脫氮塔中,NO,NO_x的平均脫除率分別提高了5.12%,5.36%.脫氮塔凈化實驗證明3LNB培養(yǎng)液能強化微生物對NO,NO_x的脫除效能,這可能主要與腐皮鐮刀菌等真菌細胞存在細胞色素P450nor有關(guān).
[Abstract]:There are many studies on the function and mechanism of bacteria at home and abroad, but less on fungi. In order to improve the removal efficiency of NO,NO_x, denitrifying fungi in biofilm of denitrification tower were isolated, purified and identified, and the action characteristics of denitrifying fungi on NO_3~- were studied by shaking flask experiment. Finally, the expanded culture medium of isolated denitrifying fungi was added to the denitrifying tower, and the effect of the isolated denitrifying fungi on the removal efficiency of NO_x was studied, and the community structure of fungi in the denitrification tower was analyzed by high throughput sequencing. The results showed that 3LNB strain was identified as Fusarium humus Fusarium solani. by morphological and nitrogen removal (NO_3~-) characteristics and ITS sequencing analysis. The results of high throughput sequencing analysis showed that there was indeed Fusarium humilis Fusarium solani. in the denitrification tower. Under the condition that the concentration of NO_3~--N was as high as 10.01g/L, the denitrification rate of bacterial denitrification group was 5.6% and the removal efficiency was 0.56 g 路L for 10 days. The denitrification rate of fungal 3LNB strain can reach 15.1%, and its removal efficiency is 1.51 g 路L ~ (- 1). Laboratory experiments showed that 3LNB strain had strong denitrification ability to NO_3~-. When the expanded culture medium 400mL (4%) of 3LNB was added to the denitrification tower, the average removal rate of NO,NO_x increased by 5.12% and 5.36%, respectively. The purification experiment of denitrification tower showed that 3LNB medium could enhance the removal efficiency of NO,NO_x by microorganisms, which may be related to the existence of cytochrome P450nor in fungal cells such as Fusarium humus.
【作者單位】： 云南大學(xué)生態(tài)學(xué)與環(huán)境學(xué)院;
【基金】：國家自然科學(xué)基金資助項目(51168046,51278447) 云南省自然科學(xué)基金資助項目(2015FB111)~~
【分類號】：X172;X701

【相似文獻】

相關(guān)期刊論文 前5條

1 陳佳儀;;亞鐵螯合劑法同時脫硫脫氮[J];科技信息;2014年09期

2 張立群;電廠燃煤鍋爐同時脫硫脫氮技術(shù)分析[J];科技情報開發(fā)與經(jīng)濟;2005年07期

3 毛永楊;鄒平;孫s釷，

本文編號：2473813