本文關(guān)鍵詞： 活性炭纖維 填埋場覆蓋層微生物 固定化細(xì)胞反應(yīng)器 三氯乙烯　出處：《應(yīng)用與環(huán)境生物學(xué)報》2017年05期 　論文類型：期刊論文

【摘要】：為探究固定化細(xì)胞對三氯乙烯(TCE)的生物降解特性及機(jī)理,以優(yōu)選的活性炭纖維為固定化材料,構(gòu)建雙塞頭固定化細(xì)胞反應(yīng)器;利用填埋場覆蓋層富集篩選的高效混合菌SWA1為生物介質(zhì)開展細(xì)胞固定化及TCE生物降解研究;運(yùn)用掃描電鏡,熒光定量PCR和高通量測序技術(shù)分別考察TCE降解前后固定化微生物微觀結(jié)構(gòu)、關(guān)鍵酶表達(dá)豐度和混合菌群落結(jié)構(gòu)變化.結(jié)果顯示:(1)該固定化細(xì)胞反應(yīng)器對微生物具有良好的固定化效果,且微生物可維持較高活性(甲烷氧化速率為0.6μmol g~(-1) d~(-1));(2)在TCE初始濃度為18 mg/L時,反應(yīng)1 d降解率為94.1%,降解過程中有8.7μmol有機(jī)氯轉(zhuǎn)化為氯離子;(3)反應(yīng)器中TCE降解關(guān)鍵酶為甲烷單加氧酶和苯酚羥化酶,TCE降解后優(yōu)勢菌屬為Anaerolineaceae_unclassified(0.6%-19.9%)和甲基孢囊菌屬M(fèi)ethylocystis(0-15.8%).該雙塞頭固定化細(xì)胞反應(yīng)器能高效去除氯代烴類污染物,可為含氯有機(jī)廢水高效生物治理的工程應(yīng)用提供參考.
[Abstract]:In order to investigate the biodegradation characteristics and mechanism of TCEs by immobilized cells, a double-plug immobilized cell reactor was constructed using the selected activated carbon fiber as the immobilized material. Cell immobilization and biodegradation of TCE were carried out by using highly efficient mixed bacteria (SWA1) enriched and screened in landfill as biomaterials. The microstructures of immobilized microorganisms before and after TCE degradation were investigated by scanning electron microscopy fluorescence quantitative PCR and high-throughput sequencing. The results showed that the immobilized cell reactor had a good immobilization effect on microorganism. The microorganism can maintain high activity (methane oxidation rate is 0. 6 渭 mol / g ~ (-1)). (2) when the initial concentration of TCE was 18 mg/L, the degradation rate for 1 day was 94.1, and there was 8.7 渭 mol organic chlorine converted to chloride ion in the degradation process. The key enzymes of TCE degradation in the reactor are methane monooxygenase and phenol hydroxylase. The dominant bacteria of the genus Anaerolineaceaeae after TCE degradation are 0.6-19.9- 0.6-19.9in the genus Anaerolineaceaeae. And Methylocystis 0-15.80.The double plug immobilized cell reactor can effectively remove chlorinated hydrocarbon pollutants. It can provide reference for the engineering application of high efficiency biological treatment of organic wastewater containing chlorine.
【作者單位】： 重慶理工大學(xué)化學(xué)化工學(xué)院;
【基金】：國家自然科學(xué)基金項(xiàng)目(51378522,41502328) 重慶市基礎(chǔ)與前沿研究項(xiàng)目(cstc2015jcyjB0015) 重慶市民生保障項(xiàng)目(cstc2015shmszx80006)資助~~
【分類號】：X703
【正文快照】： 氯代烴是一類廣泛應(yīng)用于皮革、電子、化工等行業(yè)的工業(yè)原料,由于處置不當(dāng)造成了很多地區(qū)的環(huán)境污染[1-3],對人類健康和生態(tài)環(huán)境產(chǎn)生了嚴(yán)重威脅[4].隨著國內(nèi)外在可持續(xù)發(fā)展中對生態(tài)環(huán)境提出的更高要求,采取有效途徑 消除氯代烴污染已成為環(huán)境領(lǐng)域的重要研究內(nèi)容.氯代烴類污染

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