本文關(guān)鍵詞：新型次氯酸、硫化氫熒光探針研制及其在環(huán)境分析中的應用　出處：《江蘇大學》2017年碩士論文　論文類型：學位論文

  更多相關(guān)文章： 環(huán)境分析 次氯酸 硫化氫 香豆素 熒光成像

【摘要】：目前,環(huán)境中一些污染物如次氯酸(HOCl)和硫化氫(H2S)等對環(huán)境和人類的危害性逐漸被人們所認識。HOCl是一種僅存在于溶液中的極不穩(wěn)定的弱酸,是細胞內(nèi)氧化還原反應中的重要參與者之一;人體內(nèi)的HOCl濃度過高或過低都會影響人體的健康。H2S在生物體內(nèi)是一種具有生理作用的內(nèi)源性氣體,但同時其具有極強的毒性,即使環(huán)境中濃度極低也會造成人體損傷。研制具有高性能的HOCl和H2S熒光探針仍是一個具有挑戰(zhàn)和充滿活力的領(lǐng)域。本論文中,我們合成并研究了三種以香豆素作為平臺分子的熒光探針并分別用于HOCl和H2S的檢測。在第二章中,我們以7-二乙胺基香豆素為熒光母體鍵聯(lián)強淬滅基團二硝基苯肼得到熒光探針分子Cou-HOCl-1并用于自來水和細胞內(nèi)HOCl的檢測。該探針分子由于二硝基苯肼的強淬滅熒光作用和乙酰肼中N-N單鍵的旋轉(zhuǎn)作用共同導致了其熒光背景非常弱,加入10倍當量的次氯酸時熒光強度增加了134倍,靈敏度顯著提高。探針對HOCl響應線性范圍為0.1~40μM,檢測下限為0.052μM;探針對HOCl響應快,選擇性高。最后,該探針被成功用于自來水和細胞內(nèi)HOCl的檢測。在第三章中,我們利用香豆素衍生物與巰基乙醇反應合成了熒光淬滅型次氯酸比色探針Cou-HOCl-2。加入次氯酸前后,探針分子紫外吸收光譜發(fā)生了顯著的波長變化,波長紅移了44 nm;同時溶液顏色從無色變?yōu)辄S色。當加入100μM次氯酸鈉時,探針分子的熒光淬滅了20.27倍。在次氯酸濃度為0.8~9μM范圍內(nèi),442 nm和398 nm處紫外吸光強度的比值和次氯酸濃度呈現(xiàn)良好的線性關(guān)系,檢測下限達到0.667μM。最后,該探針被用于自來水中HOCl的檢測,結(jié)果令人滿意。在第四章中,我們同樣以香豆素作為熒光母體研制了一種硫化氫(H2S)熒光探針Cou-H2S。當存在H2S時,疊氮基結(jié)構(gòu)遭到破壞轉(zhuǎn)化為氨基結(jié)構(gòu),探針Cou-H2S被激發(fā)出熒光。相對于其他活性硫化物(RSS)探針,Cou-H2S對H2S表現(xiàn)出很高的靈敏性和選擇性。當加入100μM的硫化氫(H2S)后,探針熒光強度增強了28.72倍。熒光光譜顯示,探針分子與H2S在1~10μM的濃度范圍內(nèi)呈現(xiàn)出良好的線性關(guān)系,檢測下限為0.427μM,有望用于細胞內(nèi)H2S熒光成像。
[Abstract]:Right now. Some pollutants in the environment, such as Hocl) and H2S), have been recognized as a very unstable weak acid only in solution. It is one of the important participants in intracellular redox reaction. Too high or too low concentration of HOCl in human body will affect the health of human body. H2S is an endogenous gas with physiological effect in vivo, but at the same time, it has very strong toxicity. The development of high performance HOCl and H2S fluorescent probes is still a challenging and dynamic field. We have synthesized and studied three fluorescent probes using coumarin as a platform molecule for the detection of HOCl and H2S respectively. The fluorescence probe molecule Cou-HOCl-1 was obtained by using 7-diethylaminocoumarin as the fluorescence mother with strong quenching group dinitrophenylhydrazine. The probe molecule was used for the detection of HOCl in tap water and in cells. The fluorescence background of dinitrophenylhydrazine is very weak due to the strong quenching of fluorescence and the rotation of N-N single bond in acetyl hydrazine. When 10 times equivalent hypochlorite was added, the fluorescence intensity increased by 134-fold and the sensitivity was improved significantly. The linear range of the HOCl response of the probe was 0.1 渭 m and the detection limit was 0.052 渭 M. The probe is fast in response to HOCl and highly selective. Finally, the probe has been successfully used for the detection of HOCl in tap water and cells. In Chapter 3. Cou-HOCl-2was synthesized by the reaction of coumarin derivatives with mercaptoethanol. The fluorescence quenching hypochloric acid colorimetric probe Cou-HOCl-2 was synthesized before and after the addition of hypochloric acid. The UV absorption spectrum of the probe molecule changed obviously, and the wavelength shifted to 44 nm. At the same time, the color of the solution changed from colorless to yellow. When 100 渭 M sodium hypochlorite was added, the fluorescence of the probe molecule quenched 20.27 times. The ratio of UV absorptivity to hypochloric acid concentration at 442 nm and 398 nm showed a good linear relationship, and the detection limit was 0.667 渭 M. finally. The probe was used to detect HOCl in tap water with satisfactory results. In Chapter 4th. We have also developed a novel fluorescence probe Cou-H2Swith coumarin as the fluorescence mother. In the presence of H2S, the azido group structure was destroyed and converted into amino structure. The probe Cou-H2S was stimulated by fluorescence, compared with other active sulphide probes. Cou-H2S showed high sensitivity and selectivity to H2S. The fluorescence intensity of the probe was increased by 28.72 times when 100 渭 M H2S was added. The probe molecule has a good linear relationship with H _ 2S in the concentration range of 1 ~ 10 渭 m, and the detection limit is 0.427 渭 M, which is expected to be used in intracellular H _ 2S fluorescence imaging.
【學位授予單位】：江蘇大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：X830

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