【摘要】：本文以菜籽粕為原料,酸處理將菜籽粕脫毒后,采用堿溶酸沉法提純菜籽蛋白,再用堿性蛋白酶酶解菜籽蛋白制備菜籽多肽,采用單因素逐級(jí)優(yōu)化試驗(yàn)設(shè)計(jì),以酶解液中菜籽多肽的含量為指標(biāo)優(yōu)化酶解工藝參數(shù)。結(jié)果表明:酸處理后的硫甙含量下降了84.32%,堿溶酸沉后,蛋白的提取率為76.25%。單因素逐級(jí)優(yōu)化試驗(yàn)的結(jié)果表明:堿性蛋白酶酶解的最適條件為底物濃度4%、加酶量7%(E/S)、溫度50℃、p H 8.5、酶解時(shí)間120 min,該條件下多肽含量為(35.4±0.11)mg/m L。
[Abstract]:Rapeseed protein was purified from rapeseed meal by acid treatment after detoxification, and rapeseed polypeptide was prepared by alkaline protease enzymatic hydrolysis of rapeseed protein. The experimental design was optimized by single factor step by step. The content of rapeseed polypeptide in enzymatic hydrolysis solution was taken as the index to optimize the technological parameters of enzymatic hydrolysis. The results showed that the content of glucosinolate decreased by 84.322.After alkaline acid precipitation, the extraction rate of protein was 76.25%. The results of single-factor stepwise optimization test showed that the optimum conditions for enzymatic hydrolysis of alkaline protease were substrate concentration of 4, enzyme content of 7% (E / S), temperature of 50 鈩，

本文編號(hào)：2404013