【摘要】：前言 皮膚損傷愈合的過程可分為三個階段,即炎癥期、增生期及組織重建期。中性粒細(xì)胞和巨噬細(xì)胞的滲出是該過程重要的防御機(jī)制,它們吞噬并消化細(xì)菌等外源性微粒狀物質(zhì)及死亡細(xì)胞碎片。許多研究表明在這些細(xì)胞數(shù)量減少的同時有細(xì)胞的凋亡。 細(xì)胞的程序性死亡也叫做細(xì)胞凋亡,是細(xì)胞自我毀壞的生理過程。凋亡在生物發(fā)育、維持多細(xì)胞生物體內(nèi)環(huán)境穩(wěn)定及宿主防御中起十分重要的作用。細(xì)胞凋亡的失調(diào)涉及癌癥、自身免疫疾病、神經(jīng)變性疾病及局部缺血損傷等多種疾病。 caspase是誘導(dǎo)細(xì)胞凋亡的重要的蛋白酶,有14個家族成員:caspase-1～-14。caspase既是半胱氨酸蛋白酶能以半胱氨酸為親核基團(tuán)裂解底物,又是天冬氨酸蛋白酶可將天冬氨酸C-端肽鏈酶切。caspase家族的反應(yīng)是級聯(lián)放大的。上游啟動型caspase(如caspase-8、-9)被caspase適配器活化后將激活效應(yīng)型caspase(如caspase-3、-6、-7)使細(xì)胞蛋白裂解,導(dǎo)致細(xì)胞凋亡。啟動型caspase的活化是細(xì)胞凋亡的調(diào)控點。 作為caspase家族的啟動因子,caspase-8在皮膚損傷愈合過程中起重要作用。本研究用免疫組織化學(xué)染色技術(shù)和Western blot方法檢測小鼠皮膚切創(chuàng)愈合過程中caspase-8在多核粒細(xì)胞、單核細(xì)胞及成纖維細(xì)胞中的表達(dá)并探討通過檢測caspase-8表達(dá)的規(guī)律推測皮膚損傷時間的可行性。 材料和方法 1.動物模型建立及分組 健康成年清潔級昆明系雄性小鼠33只,體重35g-40g,隨機(jī)分成11
[Abstract]:The healing process of skin injury can be divided into three stages: inflammation stage, proliferative stage and tissue reconstruction stage. The exudation of neutrophils and macrophages is an important defense mechanism in this process. They engulf and digest foreign particulate matter such as bacteria and death cell fragments. Many studies have shown that the number of these cells decreases at the same time as cell apoptosis. Programmed cell death, also known as apoptosis, is a physiological process of cell self-destruction. Apoptosis plays an important role in biological development, maintenance of environmental stability in multicellular organisms and host defense. Apoptosis disorders are associated with cancer, autoimmune diseases, neurodegenerative diseases and ischemic damage. Caspase is an important protease that induces cell apoptosis. There are 14 family members: caspase-1~-14.caspase is a cysteine protease that can use cysteine as the nucleophilic group lysate substrate. Aspartate protease can cut the C-terminal peptide chain of aspartic acid. The reaction of caspase family is amplified by cascade. When upstream caspase (such as caspase-8,-9) is activated by caspase adaptor, the activated caspase (such as caspase-3,-6,-7) cleavage the cell protein and induce cell apoptosis. Activation of priming caspase is the regulatory point of apoptosis. As the promoter of caspase family, caspase-8 plays an important role in the healing of skin injury. In this study, immunohistochemical staining and Western blot methods were used to detect caspase-8 in polymorphonuclear granulocytes (PMN) during skin wound healing in mice. The expression of caspase-8 in monocytes and fibroblasts and the feasibility of estimating the time of skin injury by detecting the expression of caspase-8. Materials and methods 1. Establishment and grouping of healthy adult Kunming male mice of clean grade, weighing 35g-40g, randomly divided into 11
【學(xué)位授予單位】：中國醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：D919

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