應用昆蟲分子系統(tǒng)學對法醫(yī)嗜尸性蠅類種屬鑒定的研究
發(fā)布時間：2018-08-01 08:20
【摘要】：目的 本課題旨在探索可用于法醫(yī)嗜尸性蠅類種屬鑒定的昆蟲分子系統(tǒng)學方法。方法 選擇線粒體DNA(mtDNA)Cytb和ND1基因，PCR擴增，聚丙烯酰胺凝膠電泳銀染顯色分型，用DNA遺傳分析儀對分型產(chǎn)物測序；利用系統(tǒng)進化學軟件和統(tǒng)計學方法對序列進行分析，建立對法醫(yī)嗜尸性蠅類進行種屬鑒定的方法。結(jié)果 對中國3個地區(qū)6種嗜尸性蠅類的mtDNA Cytb和ND1基因進行了擴增、電泳檢測和序列分析；利用統(tǒng)計學的方法，通過系統(tǒng)進化學的分析，建立了這些嗜尸性蠅類的系統(tǒng)進化關(guān)系；結(jié)合系統(tǒng)進化學的分析結(jié)果，初步建立了法醫(yī)嗜尸性蠅類種屬鑒定的方法。結(jié)論 基于mtDNA Cytb和ND1基因，初步建立了法醫(yī)嗜尸性蠅類種屬鑒定的昆蟲分子系統(tǒng)學方法。
[Abstract]:Objective to explore a molecular phylogenetic method for identification of necrophilic flies in forensic medicine. Methods Mitochondrial DNA (mtDNA) Cytb and ND1 gene were amplified by PCR, polyacrylamide gel electrophoresis (page) silver staining was used to type the genotypes, DNA genetic analyzer was used to sequence the typing products, and the sequence was analyzed by systematic chemical software and statistical method. A method for species identification of necrophilic flies in forensic medicine was established. Results the mtDNA Cytb and ND1 genes of 6 species of necrophilic flies in three regions of China were amplified, electrophoretic and sequenced. The phylogenetic relationship of these necrophagous flies was established, and the method of identifying the species of coriophilic flies by forensic medicine was preliminarily established in combination with the results of phylochemical analysis. Conclusion based on mtDNA Cytb and ND1 genes, a molecular phylogenetic method for identification of species of necrophilic flies was established.
【學位授予單位】：四川大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：D919.4
本文編號：2156943
[Abstract]:Objective to explore a molecular phylogenetic method for identification of necrophilic flies in forensic medicine. Methods Mitochondrial DNA (mtDNA) Cytb and ND1 gene were amplified by PCR, polyacrylamide gel electrophoresis (page) silver staining was used to type the genotypes, DNA genetic analyzer was used to sequence the typing products, and the sequence was analyzed by systematic chemical software and statistical method. A method for species identification of necrophilic flies in forensic medicine was established. Results the mtDNA Cytb and ND1 genes of 6 species of necrophilic flies in three regions of China were amplified, electrophoretic and sequenced. The phylogenetic relationship of these necrophagous flies was established, and the method of identifying the species of coriophilic flies by forensic medicine was preliminarily established in combination with the results of phylochemical analysis. Conclusion based on mtDNA Cytb and ND1 genes, a molecular phylogenetic method for identification of species of necrophilic flies was established.
【學位授予單位】：四川大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：D919.4
【參考文獻】
相關(guān)期刊論文 前7條
1 成新躍,周紅章,張廣學;分子生物學技術(shù)在昆蟲系統(tǒng)學研究中的應用[J];動物分類學報;2000年02期
2 張亞平,施立明;動物線粒體DNA多態(tài)性的研究概況[J];動物學研究;1992年03期
3 童富淡,汪俏梅,劉艷荷;西方蜜蜂四個亞種酯酶同工酶型和蘋果酸脫氫酶Ⅱ同工酶基因型的遺傳差異[J];動物學報;2002年06期
4 張小雷,崔銀秋,呂慧英,周慧;系統(tǒng)發(fā)育分析在古DNA研究中的應用[J];吉林大學學報(理學版);2005年05期
5 張大治,戴沛捷;蜻蜓目昆蟲DNA的提取方法研究[J];寧夏大學學報(農(nóng)業(yè)科學版);2004年03期
6 蔡繼峰,劉敏,應斌武,董建國,鄧振華,陶濤,潘洪富,張紅霞,閆紅濤,廖志鋼;成都地區(qū)四種食尸性蠅類mtDNA中COⅠ基因序列檢測[J];昆蟲學報;2005年01期
7 田英芳,黃剛,鄭哲民,魏朝明;一種簡易的昆蟲基因組DNA提取方法[J];陜西師范大學學報(自然科學版);1999年04期
相關(guān)博士學位論文 前1條
1 應斌武;法醫(yī)昆蟲種屬鑒定的分子生物學方法研究[D];四川大學;2005年
，本文編號：2156943
本文鏈接：http://www.sikaile.net/shekelunwen/gongan/2156943.html
