本文選題：缺氧預處理 + 心臟挫傷��； 參考：《山西醫(yī)科大學》2006年碩士論文

【摘要】：目的：探討缺氧預處理對大鼠鈍性心臟挫傷的保護作用及其機制。 方法：建立大鼠鈍性心臟挫傷模型，健康成年wistar大鼠60只，隨機分為缺氧預處理組、單純挫傷組(每組各24只)和正常對照組(12只)，并將每組大鼠分為2h、4h、6h、12h四個時間段。挫傷前以O_2(10％)和N_2(90％)_2的混合氣體進行全身缺氧3h，，常氧正常飼養(yǎng)24h復制缺氧預處理模型。HE染色觀察大鼠心肌組織學改變，免疫組化法檢測心肌組織中cTnT及bcl-2蛋白含量并用圖像分析儀對其進行定量分析。 結(jié)果：免疫組織化學染色(1)心臟挫傷2h可見大鼠心肌cTnT明顯脫失，隨著時間延長挫傷組cTnT脫失逐漸加重，與單純挫傷組相比缺氧預處理組cTnT的脫失顯著減輕(p＜0.05)，對照組心肌未見明顯脫失；(2)對照組有少量bcl-2表達，挫傷2h組bcl-2表達減少，隨時間延長挫傷組bcl-2表達逐漸增多，與假手術(shù)組和挫傷組相比HPC組bcl-2表達明顯增強(p＜0.05)。 結(jié)論：bcl-2的表達說明其參與了心臟挫傷后心肌損傷的形成。缺氧預處理對大鼠鈍性心肌挫傷有保護作用，表現(xiàn)為HPC能減輕心肌細胞cTnT的脫失及促進bcl-2的表達。缺氧預處理的心肌保護作用很可能是通過上調(diào)bcl-2的表達來實現(xiàn)的，但其具體機制有待進一步研究。
[Abstract]:Objective: to investigate the protective effect and mechanism of anoxic preconditioning on blunt cardiac contusion in rats. Methods: a blunt cardiac contusion model was established in rats. 60 healthy adult wistar rats were randomly divided into hypoxia preconditioning group, simple contusion group (24 rats in each group) and normal control group (12 rats). Before contusion, the mixture of O _ 2 (10%) and N _ 2 (90%) was used for 3 h of systemic hypoxia. The hypoxic preconditioning model was induced by normoxic feeding for 24 h. The histological changes of myocardium were observed by HE staining. The contents of cTnT and bcl-2 protein in myocardial tissue were detected by immunohistochemical method and quantitatively analyzed by image analyzer. Results: immunohistochemical staining (1) the myocardial cTnT loss was observed at 2h after cardiac contusion in rats, and increased gradually with time prolonging in the contusion group. Compared with the simple contusion group, the loss of cTnT in hypoxia preconditioning group was significantly reduced (p < 0. 05), but no significant loss of cTnT was found in the control group. (2) there was a small amount of bcl-2 expression in the control group, while the bcl-2 expression decreased in the contusion group for 2 hours, and the bcl-2 expression in the contusion group gradually increased with the extension of time. The expression of bcl-2 in HPC group was significantly higher than that in sham operation group and contusion group (p < 0. 05). Conclusion the expression of BCL 2 suggests that it is involved in the formation of myocardial injury after cardiac contusion. Hypoxia preconditioning has protective effect on blunt myocardial contusion in rats, which shows that HPC can attenuate the loss of cTnT and promote the expression of bcl-2 in cardiac myocytes. The myocardial protective effect of hypoxia preconditioning may be achieved by upregulating the expression of bcl-2, but its mechanism needs further study.
【學位授予單位】：山西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：D919

【參考文獻】

相關(guān)期刊論文 前10條

1 陳日;缺氧預處理心肌保護作用的研究進展[J];中國醫(yī)師雜志;2003年01期

2 黃海輝,梁后杰,胡紹毅,邊志衡,何建明;磷脂酰肌醇3激酶信號通路影響細胞凋亡在肺腺癌A549細胞群集耐藥中的作用[J];第三軍醫(yī)大學學報;2004年09期

3 蔡建輝,劉維永,易定華,羅新林,梁繼河,郝衛(wèi)亞;重度心肌挫傷的發(fā)病機制[J];第四軍醫(yī)大學學報;1999年10期

4 劉維永,蔡建輝,易定華;心臟撞擊傷生物力學致傷機制及心肌挫傷分級[J];第四軍醫(yī)大學學報;2000年05期

5 陶凌,李源,高峰,王躍民,龔衛(wèi)琴;缺血后處理對急性心肌缺血再灌注兔心臟的保護作用[J];第四軍醫(yī)大學學報;2000年06期

6 章華礎(chǔ),蕭祥熊;心肌肌鈣蛋白(cTn)檢測的臨床評價[J];放射免疫學雜志;2002年04期

7 官大威,李慶生,賈靜濤,李德祥;早期心肌挫傷超微結(jié)構(gòu)變化的實驗研究[J];中國法醫(yī)學雜志;1996年04期

8 孫凱,劉志蘇,孫權(quán);缺血后處理對肝臟缺血再灌注損傷的保護作用[J];武漢大學學報(醫(yī)學版);2004年02期

9 何蘭,唐中,張國元,凡瞿明;心肌挫傷患者血清心肌標志物變化分析[J];臨床心血管病雜志;2005年01期

10 孫勝,高鈺琪,高文祥,范明;缺氧誘導因子1與PI3K/Akt/mTOR信號轉(zhuǎn)導通路[J];生命科學;2005年04期

本文編號：2099868