本文選題：腦缺血 + 膠質原纖維酸性蛋白��； 參考：《山西醫(yī)科大學》2005年碩士論文

【摘要】：目的探討大鼠腦組織缺血后不同時間膠質原纖維酸性蛋白(Glial fibrillary acidic protein,GFAP)和熱休克蛋白70(Heat shock protein 70,HSP70)的表達,旨為法醫(yī)實踐中推斷腦組織缺血時間提供科學的參考依據(jù)。 方法建立Wistar 大鼠腦組織缺血模型,應用免疫組化S-P 法與顯微圖像分析技術檢測腦組織缺血后10min、30min、1h、3h、6h、12h、24h、48h 和正常組GFAP、HSP70 的表達,觀察是否有統(tǒng)計學意義。 結果(1)大鼠海馬區(qū)神經細胞缺血3h 可見水腫、壞死,錐體細胞受損;缺血48h 見大量錐體細胞壞死,核膜崩解、核仁消失而僅留細胞碎屑。(2)腦組織缺血3h,GFAP 在星形膠質細胞的陽性表達與正常組比較無差異; 6h 與3h 比較有差異(p 0.05);12-48h 各組間比較有顯著性差異(p 0.01)。(3)HSP70 的表達隨著缺血時間的延長而逐漸增高,HSP70 陽性細胞于腦缺血后10min 后開始出現(xiàn),隨著缺血時間的延長開始逐漸增多,缺血6h 內各組間比較無差異;6-12h 各組間比較有差異(p 0.05);24-48h各組間比較有顯著性差異(p 0.01)。 結論大鼠腦組織缺血GFAP、HSP70 陽性表達呈現(xiàn)一定規(guī)律,與缺血時間明顯相關,可作為推斷腦缺血時間的參考指標之一,在法醫(yī)檢案中具有應用價值。
[Abstract]:Objective to investigate the expression of Glial fibrillary acidic protein (GFAP) and heat shock protein 70 (Heat shock protein 70, HSP70) at different time after cerebral ischemia in rats, and to provide a scientific reference for the inference of cerebral ischemic time in forensic practice.
Methods the cerebral ischemia model of Wistar rats was established, and the immunohistochemical S-P method and microscopic image analysis technique were used to detect 10min, 30min, 1H, 3h, 6h, 12h, 24h, 48h and GFAP, HSP70, and whether there was statistical significance.
Results (1) edema, necrosis, and pyramidal cells were damaged in the ischemic 3H in the hippocampus of the rats, and the ischemic 48h saw a large number of pyramidal cells necrosis, the nuclear membrane disintegrating and the nucleolus disappeared and only the cell debris left. (2) the cerebral ischemia 3h, the positive expression of GFAP in astrocytes was not different from that in the normal group; 6h and 3H were different (P 0.05); 12-48h There were significant differences in each group (P 0.01). (3) the expression of HSP70 increased gradually with the prolongation of ischemic time. The HSP70 positive cells began to appear after 10min after cerebral ischemia, and gradually increased with the prolongation of ischemic time. There was no difference between each group in the ischemic 6h (P 0.05), and the ratio of 24-48h to each group was compared. There was a significant difference (P 0.01).
Conclusion the positive expression of GFAP and HSP70 in the cerebral ischemia of rats is related to the ischemic time. It can be used as one of the reference indexes to infer the time of cerebral ischemia, and it is valuable in forensic examination.
【學位授予單位】：山西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2005
【分類號】：D919

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