本文選題：過(guò)敏性休克　切入點(diǎn)：類胰蛋白酶　出處：《吉林大學(xué)》2005年碩士論文

【摘要】：肥大細(xì)胞(Mast Cell,MC)與變態(tài)反應(yīng)性疾病有著密切的關(guān)系,在其發(fā)病過(guò)程中發(fā)揮重要的作用。過(guò)敏性休克是臨床常見(jiàn)的急癥之一,由于缺乏確切的實(shí)驗(yàn)室診斷標(biāo)準(zhǔn),是導(dǎo)致醫(yī)療糾紛的一個(gè)重要原因。因此,研究過(guò)敏性休克發(fā)生的機(jī)制,確立可靠、準(zhǔn)確的診斷指標(biāo),提出簡(jiǎn)單易行的診斷方法,在法醫(yī)病理診斷中尤為重要。本實(shí)驗(yàn)應(yīng)用類胰蛋白酶、IL-1β免疫組化染色、甲苯胺藍(lán)特殊染色的方法觀察了27例過(guò)敏性休克致死和8例非過(guò)敏性休克致死病例的各臟器,通過(guò)研究,得到如下結(jié)論: 1.過(guò)敏性休克致死尸檢病例中各臟器肥大細(xì)胞(MC)數(shù)目增多,尤以肺組織、腸組織最明顯。 2.類胰蛋白酶可以作為MC特異性標(biāo)記檢測(cè)組織中MC的數(shù)量。 3.尸檢病例腸及肺等組織中大量嗜酸性粒細(xì)胞的存在提示過(guò)敏性休克死亡。 4、TNF-α、IL-1β參與過(guò)敏性休克的病理過(guò)程。 5、過(guò)敏性休克時(shí),腸粘膜Eotaxin染色陽(yáng)性。 本實(shí)驗(yàn)應(yīng)用免疫組化染色和特殊染色的方法,通過(guò)研究過(guò)敏性休克中MC的形態(tài)變化,證實(shí)在過(guò)敏性休克過(guò)程中類胰蛋白酶、TNF-α、IL-1β和Eotaxin發(fā)揮的作用,為其在法醫(yī)病理實(shí)踐中的應(yīng)用提供了切實(shí)的理論依據(jù)。
[Abstract]:Mast cell carcinoma (Mast) has a close relationship with allergic diseases and plays an important role in the pathogenesis of the disease. Anaphylactic shock is one of the common clinical emergencies, due to the lack of accurate laboratory diagnostic criteria. Therefore, to study the mechanism of anaphylactic shock, to establish reliable and accurate diagnostic indicators, and to put forward a simple and feasible diagnostic method. It is especially important in forensic pathology diagnosis. The organs of 27 cases of anaphylactic shock and 8 cases of non-anaphylactic shock were observed by using trypsinoid IL-1 尾 immunohistochemical staining and toluidine blue special staining. The following conclusions have been drawn:. 1. The number of mast cells in various organs was increased, especially in lung tissue and intestinal tissue. 2. Trypsin can be used as a specific marker to detect the number of MC in tissues. 3. The presence of a large number of eosinophils in intestinal and lung tissues suggests anaphylactic shock death. 4 TNF- 偽 -IL-1 尾 was involved in the pathological process of anaphylactic shock. 5. Eotaxin staining was positive in intestinal mucosa during anaphylactic shock. In this study, immunohistochemical staining and special staining were used to study the morphological changes of MC in anaphylactic shock. The results showed that the role of TNF- 偽 IL-1 尾 and Eotaxin in the process of anaphylactic shock. It provides a practical theoretical basis for its application in forensic pathology practice.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類號(hào)】：D919

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本文編號(hào)：1663155