生物樣品的檢測需要納升電噴霧離子化質(zhì)譜（nESI-MS）來適應(yīng)更苛刻的條件,如高濃度的非揮發(fā)性緩沖液和亞微米尺寸的毛細(xì)管孔徑.以上兩個需求會給nESI-MS帶來一個棘手的問題:毛細(xì)管堵塞.因此,為了延緩毛細(xì)管堵塞,我們將感應(yīng)交流電壓施加在尖端內(nèi)徑小于1μm且注入高濃度鹽溶液的毛細(xì)管上進(jìn)行電噴霧.結(jié)果表明,改進(jìn)后的毛細(xì)管比常規(guī)nESI下的毛細(xì)管壽命長1～2個數(shù)量級.較長的噴霧時間是由于電場方向周期性變化使析出的鹽結(jié)晶再溶解從而延緩毛細(xì)管堵塞.同時,經(jīng)過長時間（約10 min）高濃度鹽溶液的電噴霧,改進(jìn)后的nESI仍保持穩(wěn)定和靈敏的信號.最后對模擬細(xì)胞外液和細(xì)胞內(nèi)液進(jìn)行了驗證,結(jié)果表明感應(yīng)nESI是一種延緩毛細(xì)管堵塞的廣泛適用的生物樣品分析方法. 

【文章來源】：中國科學(xué)技術(shù)大學(xué)學(xué)報. 2020,50(11)北大核心CSCD

【文章頁數(shù)】：8 頁

【文章目錄】：
1 Introduction
2 Experimental section
3 Results and discussion
    3.1 Effects of conventional nESI and induced nESI on the tip clogging
    3.2 Dynamic status of tips in conventional and induced nESI
    3.3 Investigation of the performance of induced nESI under various conditions
    3.4 Versatility of Induced alternating voltage on mitigating the clogging of mimic intracellular fluid and extracellular fluid
4 Conclusions
Conflict of interest



本文編號：3550334