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牛支原體NM 2012株全基因組測(cè)序缺口修補(bǔ)及序列分析

發(fā)布時(shí)間:2018-05-01 23:15

  本文選題:牛支原體 + 克隆。 參考:《內(nèi)蒙古農(nóng)業(yè)大學(xué)》2015年碩士論文


【摘要】:牛支原體是能夠?qū)е屡7窝、關(guān)節(jié)炎、流產(chǎn)、乳腺炎等多種疾病的一種病原體。給養(yǎng)牛業(yè)造成了巨大的經(jīng)濟(jì)損失,嚴(yán)重影響了養(yǎng)牛業(yè)的健康發(fā)展。由于王艷杰測(cè)序的M. bovis NM 2012菌株的全基因組序列存在序列缺口和多余的重復(fù)序列,為了得到M.bovis NM 2012基因組完整序列,本試驗(yàn)對(duì)王艷杰測(cè)序的M.bovis NM 2012進(jìn)行基因組補(bǔ)缺口(補(bǔ)洞)工作。本試驗(yàn)選擇M.bovis HB0801為參考基因序列,經(jīng)過(guò)將王艷杰測(cè)序的M.bovis NM 2012與M.bovis HB0801比對(duì)后發(fā)現(xiàn),共有34處未完成的基因序列,在未完成的序列兩端各增加200~1,000bp設(shè)計(jì)引物,進(jìn)行PCR擴(kuò)增和克隆測(cè)序,并利用生物信息學(xué)軟件進(jìn)行拼接,從而完成M.bovisNM 2012菌株的全基因組補(bǔ)缺口(補(bǔ)洞)工作,并應(yīng)用生物信息學(xué)軟件對(duì)M.bovisNM 2012菌株的ORFs、脂蛋白、IS元件等進(jìn)行預(yù)測(cè),并對(duì)相關(guān)的基因進(jìn)行功能注釋。比對(duì)結(jié)果顯示,在34處未完成的基因序列中,有21處由字母N代替,字母N的長(zhǎng)度是在10~2,359bp之間,字母N的總長(zhǎng)度為13,131bp;34處未完成的序列中,有23處是缺口序列,總共缺失的序列是24,763bp,11處多余重復(fù)的序列長(zhǎng)度為14,765bp?寺y(cè)序結(jié)果表明,在23處缺口序列中,總共缺失的序列是20,222bp;11處多余重復(fù)序列的總長(zhǎng)度為14,765bp,實(shí)際有10,226bp為無(wú)效的重復(fù)序列,4,539bp確為M.bovis NM 2012本身的序列;本試驗(yàn)中,去除由未知序列N代替的13,131bp和無(wú)效的重復(fù)序列10,226bp,補(bǔ)齊缺失的序列20,222bp。將王艷杰測(cè)序的全基因組大小為993,483bp的M.bovis NM 2012,經(jīng)過(guò)補(bǔ)缺口工作,最終拼接得到M. bovis NM 2012全基因組大小為990,348bp。M.bovis NM 2012菌株全基因組大小為990,348bp, GC%含量為29.29%;與M.bovis HB0801進(jìn)行比對(duì)后,預(yù)測(cè)編碼區(qū)含839個(gè)ORFs,含有34個(gè)tRNA,6個(gè)rRNA,預(yù)測(cè)含有103個(gè)脂蛋白和2個(gè)Vsp。通過(guò)對(duì)5株牛支原體全基因組進(jìn)行基因組共線性分析比較,M. bovis NM 2012基因組與M.bovis PG45基因組的相似性最小,與M.bovis HB0801基因組的相似性最高。
[Abstract]:Mycoplasma bovis is a pathogen that can cause many diseases, such as pneumonia, arthritis, abortion, mastitis and so on. It has caused enormous economic losses to the cattle industry and seriously affected the healthy development of the cattle industry. In order to obtain the complete genome sequence of M.bovis NM 2012, there is a gap in the whole genome sequence and redundant repeat sequence in the whole genome sequence of M. bovis NM 2012, which was sequenced by Wang Yanjie. M.bovis NM 2012, which was sequenced by Wang Yanjie, was studied in this experiment. In this experiment, M.bovis HB0801 was selected as the reference gene sequence. After comparing the M.bovis NM 2012 and M.bovis HB0801 sequenced by Wang Yanjie, it was found that there were 34 uncompleted gene sequences, and the primers designed by 200~1000bp were added at each end of the unfinished sequence. PCR amplification, cloning and sequencing were carried out, and bioinformatics software was used to assemble the whole genome gap (hole) of M.bovisNM 2012 strain. Bioinformatics software was used to predict ORFs, lipoprotein is elements of M.bovisNM 2012 strain, and to annotate the related genes. The results showed that of the 34 uncompleted sequences, 21 were replaced by the letter N, the length of the letter N was between 10~2359bp, and the total length of the letter N was 13131bpng34, of which 23 were gap sequences. The total missing sequence was 24763bpmpn11, and the length of the redundant repeats was 14765bp. The results of cloning and sequencing showed that the total missing sequence of 23 gap sequences was 20222bp-1, the total length of redundant repeats was 14765 BP, and the 4539bp repeats with 10226bp as invalid were indeed the sequences of M.bovis NM 2012. The 13131bp replaced by the unknown sequence N and the invalid repeat sequence 10226bpwere removed, and the missing sequence 20222bpwas added. The whole genome size of M.bovis NM2012, which was 993483bp, was sequenced by Wang Yanjie. After filling the gap, the whole genome size of M. bovis NM 2012 strain 990348bp.M.bovis NM 2012 was 990348bpand the content of GC% was 29.290.After comparing with M.bovis HB0801, the whole genome size of M. bovis NM 2012 was 990348bpand the content of GC% was 29.29. The predicted coding region contained 839 ORFs, 34 tRNAs, 6 rRNAs, 103 lipoproteins and 2 Vsps. The genomic collinear analysis of 5 strains of Mycoplasma bovis was conducted to compare the similarity between M. bovis NM 2012 genome and M.bovis PG45 genome, and the highest similarity with M.bovis HB0801 genome.
【學(xué)位授予單位】:內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】:碩士
【學(xué)位授予年份】:2015
【分類號(hào)】:S852.62

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 胡長(zhǎng)敏;石磊;龔瑞;白智迪;郭愛珍;;牛支原體病研究進(jìn)展[J];動(dòng)物醫(yī)學(xué)進(jìn)展;2009年08期

2 張利;李玉平;黎曉敏;尼瑪倉(cāng)木拉;;牛支原體藥物敏感性試驗(yàn)[J];動(dòng)物醫(yī)學(xué)進(jìn)展;2012年02期

3 陳忠瓊;范媛;黎曉敏;;牛支原體生物學(xué)特性及其膜蛋白的研究進(jìn)展[J];中國(guó)畜牧獸醫(yī);2012年02期

4 石磊;龔瑞;尹爭(zhēng)艷;周勇;裴潔;胡智斌;王利霞;胡長(zhǎng)敏;劉濤;陳穎鈺;廖娟紅;趙俊龍;陳煥春;郭愛珍;;肉牛傳染性牛支原體肺炎流行的初步診斷[J];華中農(nóng)業(yè)大學(xué)學(xué)報(bào);2008年04期

5 蔣勇;周蓓;黎曉敏;;牛支原體病診斷方法的研究進(jìn)展[J];飼料博覽;2013年04期

6 李大偉;黃燦平;張彥明;謝建華;冉智光;熊仲良;范偉興;;牛支原體、無(wú)乳支原體和絲狀支原體絲狀亞種小克隆三重PCR檢測(cè)方法的建立及初步應(yīng)用[J];畜牧獸醫(yī)學(xué)報(bào);2011年02期

7 張永強(qiáng);張維;王清華;吳研功;趙云玲;趙永剛;王志亮;;牛支原體肺炎流行RT-PCR方法的初步診斷[J];中國(guó)動(dòng)物檢疫;2011年06期

8 張軒;儲(chǔ)岳峰;逯忠新;;家畜重要支原體病疫苗的研究進(jìn)展[J];中國(guó)獸醫(yī)科學(xué);2011年12期

9 辛九慶;李媛;郭丹;宋念華;胡守萍;陳超;裴潔;曹培麗;;國(guó)內(nèi)首次從患肺炎的犢牛肺臟中分離到牛支原體[J];中國(guó)預(yù)防獸醫(yī)學(xué)報(bào);2008年09期

10 宋志強(qiáng);李媛;孫文靜;劉洋;鄒曉輝;陳維;周玉梅;辛九慶;;牛支原體新基因(P18)的克隆表達(dá)及活性鑒定[J];中國(guó)預(yù)防獸醫(yī)學(xué)報(bào);2012年03期

相關(guān)碩士學(xué)位論文 前5條

1 李彥芹;牛支原體PCR檢測(cè)方法的建立及臨床應(yīng)用[D];山東農(nóng)業(yè)大學(xué);2009年

2 范媛;牛支原體生長(zhǎng)特性及其致病性研究[D];西南大學(xué);2011年

3 孫文靜;牛支原體硫辛酰胺脫氫酶的蛋白質(zhì)組學(xué)篩選及其功能鑒定[D];東北農(nóng)業(yè)大學(xué);2012年

4 蔣勇;牛支原體免疫原性研究[D];西南大學(xué);2013年

5 王艷杰;牛支原體的分離鑒定及其全基因組序列測(cè)定與分析[D];內(nèi)蒙古農(nóng)業(yè)大學(xué);2014年

,

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