魚(yú)類性別控制研究是現(xiàn)代水產(chǎn)養(yǎng)殖的熱點(diǎn)。金錢(qián)魚(yú)（Scatophagus argus,Linnaeus,1766）是一種廣鹽性魚(yú)類,可作觀賞魚(yú)和食用魚(yú),在養(yǎng)殖群體中性比為1:1。金錢(qián)魚(yú)雌魚(yú)的生長(zhǎng)比雄魚(yú)快,個(gè)體大,有更好經(jīng)濟(jì)價(jià)值。因此,克隆性別決定主效基因（master sex-determination gene,SD）將有助于金錢(qián)魚(yú)的性別控制。本研究旨在研究金錢(qián)魚(yú)的性別決定和分化機(jī)制,并為以后的單性（全雌魚(yú)）金錢(qián)魚(yú)培育提供理論基礎(chǔ)。下面為本研究的主要發(fā)現(xiàn):1、金錢(qián)魚(yú)性別特異性標(biāo)記的開(kāi)發(fā)和性別決定候選基因的克隆本研究克隆了金錢(qián)魚(yú)Dmrt1（Dmrt1和Dmrt1b）和Dmrt3（Dmrt3和Dmrt3△-Y）的兩個(gè)亞型。本研究開(kāi)發(fā)了兩個(gè)位于金錢(qián)魚(yú)Dmrt3和Dmrt1上的性別連鎖分子標(biāo)記,分別命名為Dmrt3-Marker-F/R和Dmrt1-Marker-F/R。Dmrt3-Marker-F/R擴(kuò)增的片段包含能導(dǎo)致截短型Dmrt3（Dmrt3△-Y）c DNA提前編碼終止的基因組序列。Dmrt3-Marker-F/R擴(kuò)增片段的151和166 bp單核苷酸多態(tài)性（SNP）顯示雌性個(gè)體均為C... 

【文章來(lái)源】：廣東海洋大學(xué)廣東省

【文章頁(yè)數(shù)】：121 頁(yè)

【學(xué)位級(jí)別】：碩士

【文章目錄】：
Abbreviations
Abstract
摘要
1 General Introduction
    1.1 Background of the Study
    1.2 Literature Review on Teleost Sex Determination and Differentiation (SDD)
        1.2.1 Processes of Gonad Development in Gonochoristic
        1.2.2 Sex-Determination (SD) Genes in Fish
        1.2.3 Other Sex Related Genes
        1.2.4 Epigenetic Mechanisms
        1.2.5 Environmental Effects (EE) on Sex Determination and Differentiation (SDD)
        1.2.6 Understanding the Sex-Determination (SD) Pathway
        1.2.7 The Tag of War Between GSD and ESD
        1.2.8 Sex Control in Fish
    1.3 Problem Statement
    1.4 The Aim and Scope of the Study
    1.5 The Scientific Significance of the Study
2 Identification of Sex-Specific Markers and Cloning of Candidate Sex-Determination Gene in Spotted scat, Scatophagus argus
    2.1 Introduction
    2.2 Materials and Methods
        2.2.1. Animals and Samples Collection
        2.2.2 RNA Extraction
        2.2.3 Genomic DNA (gDNA) Extraction
        2.2.4 Cloning of cDNA and gDNA
        2.2.5 Sequence Analysis
        2.2.6 Syntenic Analysis
        2.2.7 Reverse Transcription PCR (RT-PCR)
    2.3 Results
        2.3.1 cDNA Cloning and Sequence Analysis
        2.3.2 Phylogenetic Analysis
        2.3.3 Screening of Sex-Specific Marker on Dmrt3 Locus
        2.3.4 Cloning of Male-Specific Dmrt1
        2.3.5 Synthenic Analysis
        2.3.6 Gene Expression Profile of Dmrt1, Dmrt1b, Dmrt3 and Dmrt3△-Y in Gonads of Scatophagus argus
    2.4 Discussion
    2.5 Conclusion
3 Expression Analysis and Relationship Study of Dmrt1 and Gsdf in Spotted Scat(Scatophagus argus)
    3.1 Introduction
    3.2 Materials and Methods
        3.2.0 Animals and Sampling
        3.2.1 Genotyping and Phenotyping of the Sex
        3.2.2 H&E Staining
        3.2.3 cDNA Cloning and Sequence Analysis
        3.2.4 Tissue Distribution of gsdf, dmrt1 and sf1 mRNA
        3.2.5 Expression of gsdf, dmrt1 and sf1 at Different Gonad Developmental Phases
        3.2.6 Western Blotting
        3.2.7 Immunohistochemistry (IHC)
        3.2.8 Primary Antibody Pre-adsorption Test
        3.2.9 In Silico and In Vitro Promoter Analysis
        3.2.10 Statistical Analysis
    3.3 Results
        3.3.1 Fish Sampling
        3.3.2 cDNA Cloning and Sequence Analysis
        3.3.3 Tissue Distribution
        3.3.4 Expression of gsdf, dmrt1 and sf1 mRNA at Different Gonad Developmental Phases
        3.3.5 Gsdf Protein Expression in Gonads by Western Blot
        3.3.6 Expression of Gsdf at Different Gonad Developmental Phases by IHC
        3.3.7 Transcriptional Regulation of gsdf
    3.4 Discussion
        3.4.1 Gsdf Showed Sex-biased Expression in Fish and Activated by Dmrt1 in Male Spotted Scat
        3.4.2 Function of Gsdf in Ovary Development and Other Possible Factors Influence Gsdf Expression
    3.5 Conclusions
4 General Conclusion and Recommendations
    4.1 Conclusion
    4.2 Recommendations
References
Acknowledgements
Author’s profile
Supervisors’ profile


【參考文獻(xiàn)】：
期刊論文
[1]Sequencing, de novo assembly and characterization of the spotted scat Scatophagus argus（Linnaeus 1766） transcriptome for discovery of reproduction related genes and SSRs[J]. 楊尉,陳華譜,崔雪凡,張克偉,江東能,鄧思平,朱春華,李廣麗.  Journal of Oceanology and Limnology. 2018(04)
[2]金錢(qián)魚(yú)抗繆勒氏管激素基因克隆及其在性腺發(fā)育不同時(shí)期mRNA表達(dá)水平的分析[J]. 曾文剛,劉振浩,李紅,張俊彬.  水產(chǎn)學(xué)報(bào). 2015(11)
[3]金錢(qián)魚(yú)性腺發(fā)育及其組織結(jié)構(gòu)觀察[J]. 崔丹,劉志偉,劉南希,張瑩瑩,張俊彬.  水產(chǎn)學(xué)報(bào). 2013(05)



本文編號(hào)：3593443