高糖高脂日糧對團頭魴生長性狀、肝組織結構及轉錄組的影響
發(fā)布時間：2021-11-01 23:31
團頭魴是中國重要的淡水草食性混養(yǎng)魚類之一。近年來,如何最大限度地利用非蛋白能量飼料及其在飼料中如何正確配比等問題已有較多研究與報道。非蛋白能量飼料的過量使用可以造成魚類體脂的大量沉積,從而導致魚類脂肪肝疾病。然而迄今為止,有關非蛋白能量飼料過量使用導致團頭魴脂肪肝疾病發(fā)生的相關報道較少。通常情況下,魚類攝食是由餌料能量及營養(yǎng)成分等因素協(xié)同調節(jié)影響魚類健康,而魚類健康又與相關功能基因的表達有關,類似的研究已在其它魚類中獲得證實,如虹鱒、金鯧等。團頭魴該方面的相關研究,主要集中在碳水化合物與脂肪飼料對團頭魴生長性能及免疫機制改善等方面的研究,而由于日糧中高糖高脂導致團頭魴脂肪沉積所產(chǎn)生的生長性能和肝組織改變,以及其變化后的轉錄組學特征未見報道。因此,本文以團頭魴為研究對象,經(jīng)過8周高糖高脂日糧的飼養(yǎng)實驗,利用組織切片與轉錄組學的方法來研究高脂日糧（HFD,脂肪含量12.22%）、高糖日糧（HCBD,還原糖含量34.13%）和高脂高糖日糧（HFHCD,脂肪含量10.43%,還原糖含量30.80%）對團頭魴生長性能、肝組織及肝轉錄組的影響,并在此基礎上,結合實時定量PCR、血液生化指標及部分肝...
【文章來源】：華中農(nóng)業(yè)大學湖北省 211工程院校 教育部直屬院校
【文章頁數(shù)】：154 頁
【學位級別】：博士
【文章目錄】：
中文摘要
ABSTRACT
ABBREVIATIONS
CHAPTER 1 Review of literature
1.1 General introduction of Megalobrama amblycephala
1.1.1 Biological characteristics
1.1.2 Megalobrama amblycephala nutrient requirements
1.1.3 The problem of Megalobrama amblycephala in productions
1.2 Transcriptome study
1.2.1 Introduction of transcriptome
1.2.2 The ability of transcriptomics
1.2.3 The use of transcriptome data to explore gene functions
1.2.4 Transcriptome studies in aquatic animals
1.2.5 The study of transcriptome in Megalobrama amblycephala
1.3 Fat and carbohydrate in fish diets
1.3.1 Fat and carbohydrate characteristics
1.3.2 Function in animals
1.3.3 Fat and carbohydrate diets in aquaculture
1.4 Fatty liver disease
1.4.1 Fatty liver disease types
1.4.2 Fatty liver disease in fish researches
1.5 Objective and significance
1.5.1 Objective
1.5.2 Significance
CHAPTER 2 The effect of high fat and high carbohydrate diets on growth and liver histology of Megalobrama amblycephala
2.1 Introduction
2.2 Materials and methods
2.2.1 Animal rearing and experimental procedures
2.2.2 Dietary preparation and proximate analysis
2.2.3 Sample collection
2.2.4 Histology
2.3 Results
2.3.1 The effect of high fat and high carbohydrate diets on growth performance
2.3.2 Liver histology changes of BSB fed with high fat and high carbohydrate diets
2.4 Discussion
CHAPTER 3 The overview of transcriptome analysis fed with high fat and high carbohydrate diets in Megalobrama amblyphyla
3.1 Introduction
3.2 Materials and methods
3.2.1 Sampling and RNA extraction
3.2.2 c DNA library preparation and Illumina sequencing
3.2.3 De novo assembly of sequencing reads
3.2.4 Annotation
3.2.5 Gene expression analysis
3.2.6 SSR markers
3.3 Results
3.3.1 Transcriptome sequencing and assembly
3.3.2 Blast search analysis and unigene functional annotation
3.3.3 The differentially expressed gene profile
3.3.4 Pathway analysis
3.3.5 SSR markers
3.4 Discussion
CHAPTER 4 High Carbohydrate Diet Negatively Affects the Liver Health of Megalobrama amblycephala
4.1 Introduction
4.2 Materials and methods
4.2.1 Diet preparation and proximate analysis
4.2.2 Animal rearing, experimental procedures and sample collection
4.2.3 Metabolomics study by NMR
4.2.4 Transcriptome analysis
4.2.5 qRT-PCR analysis
4.3 Results
4.3.1 Hepatosomatic index and liver histology
4.3.2 Serum biochemistry
4.3.3 The metabolomics of serum and liver
4.3.4 Impacts of high-carbohydrate diet on the expressions of DEGs associated with NAFLD and insulin signaling pathways
4.4 Discussions
4.4.1 High-carbohydrate induced hepatosomatic index and liver histology changes
4.4.2 High-carbohydrate induced changes in serum biochemistry
4.4.3 High-carbohydrate induced fatty liver associated changes in serum metabolomics and liver metabolomics
4.4.4 The expression of DEGs in NAFLD and insulin signaling pathways via transcriptome and qRT-PCR
CHAPTER 5 High-fat-high-carbohydrate diet causes mitochondrial dysfunction in the liver of Megalobrama amblycephala
5.1 Introduction
5.2 Materials and methods
5.2.1 Diet preparation and proximate analysis
5.2.2 Animal rearing, feeding and sample collection
5.2.3 Transcriptome analysis
5.2.3.1 RNA extraction
5.2.3.2 cDNA library preparation and Illumina sequencing
5.2.3.3 De novo assembly of sequencing reads
5.2.3.4 Annotation
5.2.3.5 Ontology analysis
5.2.3.6 Gene expression analysis
5.2.4 qRT-PCR analysis
5.3 Results
5.3.1 Transcriptome sequencing and annotation of unigenes
5.3.2 Differential expression analysis
5.3.3 qRT-PCR
5.4 Discussion
CONCLUSIONS
REFERENCES
APPENDIX 1
Fig. S1 Metabolomics graphs
Fig. S2 ~1H NMR spectra
Fig. S3 KEGG pathways
Fig. S4 Alzheimer’s disease
Fig. S5 Huntington’s disease
Fig. S6 Parkinson’s disease
Table S1. ~1H NMR signal assignment of metabolites in serum and liver extracts
Table S2. Differentially expressed genes
APPENDIX 2 Publications
APPENDIX 3 Awards and Funding
ACKNOWLEDGEMENTS
【參考文獻】：
期刊論文
[1]飼料蛋白質脂肪比與不同生長階段團頭魴全魚蛋白質、脂肪含量及肌肉氨基酸、脂肪酸組成的關系[J]. 姚林杰,葉元土,蔡春芳,許凡,劉猛,劉漢超,董嬌嬌,陳科全,黃雨薇. 動物營養(yǎng)學報. 2014(08)
[2]Microsatellite analysis of variation among wild,domesticated,and genetically improved populations of blunt snout bream（Megalobrama amblycephala）[J]. Shou-Jie TANG,Si-Fa LI,Wan-Qi CAI,Yan ZHAO. Zoological Research. 2014(02)
[3]團頭魴3個地理種群雜交效果的配合力和微衛(wèi)星標記預測[J]. 曾聰,張耀,曹小娟,羅偉,劉肖蓮,高澤霞,錢雪橋,王衛(wèi)民. 水產(chǎn)學報. 2012(06)
[4]飼料中豆粕蛋白替代魚粉蛋白對齊口裂腹魚幼魚生長性能、體成分及血液生化指標的影響[J]. 向梟,周興華,陳建,李代金,王文娟,周小秋. 水產(chǎn)學報. 2012(05)
[5]Genetically modified mouse models for the study of nonalcoholic fatty liver disease[J]. Perumal Nagarajan,M Jerald Mahesh Kumar,Ramasamy Venkatesan,Subeer S Majundar,Ramesh C Juyal. World Journal of Gastroenterology. 2012(11)
[6]團頭魴野生、馴養(yǎng)、選育3類遺傳生態(tài)群體遺傳變異的線粒體DNA分析[J]. 唐首杰,李思發(fā),蔡完其. 中國水產(chǎn)科學. 2011(03)
[7]團頭魴對營養(yǎng)需求的研究進展[J]. 張媛媛,劉波,周傳朋,戈賢平,謝駿,徐跑. 安徽農(nóng)業(yè)科學. 2010(32)
[8]利用RAPD指紋分析技術分析3種鯉科魚群體的遺傳變異[J]. 張金洲,項智鋒,李學斌,王清華,張廷虎,趙海濤,郭玉嬌. 安徽農(nóng)業(yè)科學. 2008(24)
[9]淤泥湖、梁子湖、鄱陽湖團頭魴mtDNA序列變異及遺傳結構分析[J]. 李弘華. 淡水漁業(yè). 2008(04)
[10]Pathogenesis and management issues for non-alcoholic fatty liver disease[J]. Marko Duvnjak,Ivan Leroti,Neven Bari,Vedran Tomai,Lucija Virovi Juki,Vedran Velagi. World Journal of Gastroenterology. 2007(34)
本文編號：3470893
【文章來源】：華中農(nóng)業(yè)大學湖北省 211工程院校 教育部直屬院校
【文章頁數(shù)】：154 頁
【學位級別】：博士
【文章目錄】：
中文摘要
ABSTRACT
ABBREVIATIONS
CHAPTER 1 Review of literature
1.1 General introduction of Megalobrama amblycephala
1.1.1 Biological characteristics
1.1.2 Megalobrama amblycephala nutrient requirements
1.1.3 The problem of Megalobrama amblycephala in productions
1.2 Transcriptome study
1.2.1 Introduction of transcriptome
1.2.2 The ability of transcriptomics
1.2.3 The use of transcriptome data to explore gene functions
1.2.4 Transcriptome studies in aquatic animals
1.2.5 The study of transcriptome in Megalobrama amblycephala
1.3 Fat and carbohydrate in fish diets
1.3.1 Fat and carbohydrate characteristics
1.3.2 Function in animals
1.3.3 Fat and carbohydrate diets in aquaculture
1.4 Fatty liver disease
1.4.1 Fatty liver disease types
1.4.2 Fatty liver disease in fish researches
1.5 Objective and significance
1.5.1 Objective
1.5.2 Significance
CHAPTER 2 The effect of high fat and high carbohydrate diets on growth and liver histology of Megalobrama amblycephala
2.1 Introduction
2.2 Materials and methods
2.2.1 Animal rearing and experimental procedures
2.2.2 Dietary preparation and proximate analysis
2.2.3 Sample collection
2.2.4 Histology
2.3 Results
2.3.1 The effect of high fat and high carbohydrate diets on growth performance
2.3.2 Liver histology changes of BSB fed with high fat and high carbohydrate diets
2.4 Discussion
CHAPTER 3 The overview of transcriptome analysis fed with high fat and high carbohydrate diets in Megalobrama amblyphyla
3.1 Introduction
3.2 Materials and methods
3.2.1 Sampling and RNA extraction
3.2.2 c DNA library preparation and Illumina sequencing
3.2.3 De novo assembly of sequencing reads
3.2.4 Annotation
3.2.5 Gene expression analysis
3.2.6 SSR markers
3.3 Results
3.3.1 Transcriptome sequencing and assembly
3.3.2 Blast search analysis and unigene functional annotation
3.3.3 The differentially expressed gene profile
3.3.4 Pathway analysis
3.3.5 SSR markers
3.4 Discussion
CHAPTER 4 High Carbohydrate Diet Negatively Affects the Liver Health of Megalobrama amblycephala
4.1 Introduction
4.2 Materials and methods
4.2.1 Diet preparation and proximate analysis
4.2.2 Animal rearing, experimental procedures and sample collection
4.2.3 Metabolomics study by NMR
4.2.4 Transcriptome analysis
4.2.5 qRT-PCR analysis
4.3 Results
4.3.1 Hepatosomatic index and liver histology
4.3.2 Serum biochemistry
4.3.3 The metabolomics of serum and liver
4.3.4 Impacts of high-carbohydrate diet on the expressions of DEGs associated with NAFLD and insulin signaling pathways
4.4 Discussions
4.4.1 High-carbohydrate induced hepatosomatic index and liver histology changes
4.4.2 High-carbohydrate induced changes in serum biochemistry
4.4.3 High-carbohydrate induced fatty liver associated changes in serum metabolomics and liver metabolomics
4.4.4 The expression of DEGs in NAFLD and insulin signaling pathways via transcriptome and qRT-PCR
CHAPTER 5 High-fat-high-carbohydrate diet causes mitochondrial dysfunction in the liver of Megalobrama amblycephala
5.1 Introduction
5.2 Materials and methods
5.2.1 Diet preparation and proximate analysis
5.2.2 Animal rearing, feeding and sample collection
5.2.3 Transcriptome analysis
5.2.3.1 RNA extraction
5.2.3.2 cDNA library preparation and Illumina sequencing
5.2.3.3 De novo assembly of sequencing reads
5.2.3.4 Annotation
5.2.3.5 Ontology analysis
5.2.3.6 Gene expression analysis
5.2.4 qRT-PCR analysis
5.3 Results
5.3.1 Transcriptome sequencing and annotation of unigenes
5.3.2 Differential expression analysis
5.3.3 qRT-PCR
5.4 Discussion
CONCLUSIONS
REFERENCES
APPENDIX 1
Fig. S1 Metabolomics graphs
Fig. S2 ~1H NMR spectra
Fig. S3 KEGG pathways
Fig. S4 Alzheimer’s disease
Fig. S5 Huntington’s disease
Fig. S6 Parkinson’s disease
Table S1. ~1H NMR signal assignment of metabolites in serum and liver extracts
Table S2. Differentially expressed genes
APPENDIX 2 Publications
APPENDIX 3 Awards and Funding
ACKNOWLEDGEMENTS
【參考文獻】：
期刊論文
[1]飼料蛋白質脂肪比與不同生長階段團頭魴全魚蛋白質、脂肪含量及肌肉氨基酸、脂肪酸組成的關系[J]. 姚林杰,葉元土,蔡春芳,許凡,劉猛,劉漢超,董嬌嬌,陳科全,黃雨薇. 動物營養(yǎng)學報. 2014(08)
[2]Microsatellite analysis of variation among wild,domesticated,and genetically improved populations of blunt snout bream（Megalobrama amblycephala）[J]. Shou-Jie TANG,Si-Fa LI,Wan-Qi CAI,Yan ZHAO. Zoological Research. 2014(02)
[3]團頭魴3個地理種群雜交效果的配合力和微衛(wèi)星標記預測[J]. 曾聰,張耀,曹小娟,羅偉,劉肖蓮,高澤霞,錢雪橋,王衛(wèi)民. 水產(chǎn)學報. 2012(06)
[4]飼料中豆粕蛋白替代魚粉蛋白對齊口裂腹魚幼魚生長性能、體成分及血液生化指標的影響[J]. 向梟,周興華,陳建,李代金,王文娟,周小秋. 水產(chǎn)學報. 2012(05)
[5]Genetically modified mouse models for the study of nonalcoholic fatty liver disease[J]. Perumal Nagarajan,M Jerald Mahesh Kumar,Ramasamy Venkatesan,Subeer S Majundar,Ramesh C Juyal. World Journal of Gastroenterology. 2012(11)
[6]團頭魴野生、馴養(yǎng)、選育3類遺傳生態(tài)群體遺傳變異的線粒體DNA分析[J]. 唐首杰,李思發(fā),蔡完其. 中國水產(chǎn)科學. 2011(03)
[7]團頭魴對營養(yǎng)需求的研究進展[J]. 張媛媛,劉波,周傳朋,戈賢平,謝駿,徐跑. 安徽農(nóng)業(yè)科學. 2010(32)
[8]利用RAPD指紋分析技術分析3種鯉科魚群體的遺傳變異[J]. 張金洲,項智鋒,李學斌,王清華,張廷虎,趙海濤,郭玉嬌. 安徽農(nóng)業(yè)科學. 2008(24)
[9]淤泥湖、梁子湖、鄱陽湖團頭魴mtDNA序列變異及遺傳結構分析[J]. 李弘華. 淡水漁業(yè). 2008(04)
[10]Pathogenesis and management issues for non-alcoholic fatty liver disease[J]. Marko Duvnjak,Ivan Leroti,Neven Bari,Vedran Tomai,Lucija Virovi Juki,Vedran Velagi. World Journal of Gastroenterology. 2007(34)
本文編號：3470893
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