【摘要】：目的調(diào)查一組60株大腸埃希菌臨床連續(xù)分離株常見耐藥元件的攜帶情況和菌株間的親緣性。方法收集2015年10月-12月醫(yī)院住院患者分離的60株大腸埃希菌臨床連續(xù)分離株,采用K-B法測定12種抗菌藥物的敏感性,再用聚合酶鏈反應(yīng)(PCR)及序列分析法分析15種β-內(nèi)酰胺類、6種氨基糖苷類、3種磺胺類耐藥相關(guān)基因以及4種可移動遺傳元件遺傳標(biāo)記,并對檢測結(jié)果作樣本聚類分析。結(jié)果 60株大腸埃希菌對氨芐西林、慶大霉素、妥布霉素、環(huán)丙沙星、磺胺甲VA唑/甲氧芐啶耐藥率50%,對其它7種抗菌藥物的耐藥率15%,對碳青霉烯類均敏感;60株菌共檢出常見耐藥元件基因15種,其中43株檢出β-內(nèi)酰胺類耐藥基因,檢出率為71.7%,33株檢出氨基糖苷類耐藥基因,檢出率為55.0%,40株檢出磺胺類耐藥基因,檢出率為66.7%,42株檢出可移動遺傳元件遺傳標(biāo)記基因,檢出率為70.0%;共檢出6種β-內(nèi)酰胺類耐藥基因,4種氨基糖苷類耐藥基因,3種磺胺類耐藥基因,2種可移動遺傳元件遺傳標(biāo)記基因;樣本聚類分析提示本組菌疑似存在8個克隆株,同一克隆內(nèi)菌株攜帶著相同耐藥元件,存在醫(yī)院Qg感染。結(jié)論 blaTEM、aac(3)-Ⅱ、sul1、dfrA17、intⅠ1是導(dǎo)致本組菌株對β-內(nèi)酰胺類、氨基糖苷類和磺胺類藥物耐藥的重要原因,耐藥表型與基因型相符率高。
[Abstract]:Objective to investigate the common drug resistant elements and the relationship between 60 clinical isolates of Escherichia coli. Methods A total of 60 consecutive clinical isolates of Escherichia coli from October to December 2015 were collected and the sensitivity of 12 antimicrobial agents was determined by K-B method. Polymerase chain reaction (PCR) and sequence analysis were used to analyze 15 尾 -lactams, 6 aminoglycosides, 3 sulfonamides resistance related genes and 4 transportable genetic elements. Results 60 strains of Escherichia coli were resistant to ampicillin, gentamycin, tobramycin, ciprofloxacin, sulfamethoxazole / trimethoprim. Fifteen common resistant element genes were detected in 60 strains, among which 43 strains were detected 尾 -lactam resistance genes. The detection rate was 71.7% and 33 strains detected aminoglycoside resistance genes. The detection rate was 55.0%. 40 strains of sulfanilamide resistant genes were detected, and the detectable rate was 66.7% and 42 strains, the detectable rate was 70.010%. A total of 6 尾 -lactam resistant genes, 4 aminoglycoside resistant genes, 3 sulfonamides resistance genes and 2 transportable genetic element genetic marker genes were identified. Cluster analysis showed that 8 clones were suspected to exist in this group, and the strains in the same clone were carrying the same drug resistant elements, and there was nosocomial Qg infection. Conclusion blaTEM,aac (3)-鈪，

本文編號：2407225