【摘要】：目的發(fā)展一種在生理流動(dòng)條件下分析血小板黏附聚集的簡(jiǎn)易微流控芯片技術(shù)。方法微流控分析芯片基本結(jié)構(gòu)由直微通道及兩側(cè)的樣品池和出口組成;利用理論計(jì)算和有限元數(shù)值軟件ANSYS對(duì)微通道流體動(dòng)力學(xué)行為進(jìn)行分析;微通道用Ⅰ型膠原蛋白修飾,分別在200 s~(-1)靜脈生理性剪切率和1000 s~(-1)動(dòng)脈生理性剪切率條件下使血液流過(guò)微通道,同時(shí)通過(guò)熒光顯微攝像動(dòng)態(tài)記錄血液中熒光標(biāo)記血小板與Ⅰ型膠原蛋白表面的黏附聚集圖像。結(jié)果理論和數(shù)值分析顯示,700μm×70μm(寬深比10∶1)的微通道具有最優(yōu)的流體剪切率大小分布;相比200 s~(-1)低剪切率,在1000 s~(-1)剪切率條件下血小板初始黏附時(shí)間、聚集速率和最大表面覆蓋面積均顯著降低(P0.05);在1000 s~(-1)剪切率條件下,抗凝藥物替羅非班處理呈濃度依賴(lài)性地降低血小板表面聚集率,IC50值為23.8 nmol/L。結(jié)論該微流控芯片技術(shù)可在生理相關(guān)性流體剪切率環(huán)境下動(dòng)態(tài)分析血小板黏附聚集,血樣少,方法簡(jiǎn)單易行,未來(lái)可用于血小板功能臨床檢測(cè)、抗凝藥物藥效和小型動(dòng)物模型凝血分析。
[Abstract]:Objective to develop a simple microfluidic chip for the analysis of platelet adhesion and aggregation under physiological flow conditions. Methods the basic structure of microfluidic analysis chip was composed of straight microchannel and sample cell and outlet on both sides. The hydrodynamic behavior of microchannel was analyzed by theoretical calculation and finite element software ANSYS, and the microchannel was modified with collagen 鈪，

本文編號(hào)：2276799