本文選題：睡眠時相剝奪 + 小平臺水環(huán)境��； 參考：《中華男科學(xué)雜志》2017年08期

【摘要】：目的:研究深慢波睡眠剝奪對大鼠睪丸組織氧化應(yīng)激反應(yīng)的影響。方法:36只5周齡健康雄性Wistar大鼠,隨機分為深慢波睡眠時相剝奪組(SD1組)、深慢波睡眠時相及時長剝奪組(SD2組)和空白對照組(CC組),每組12只,利用小平臺水環(huán)境建立深慢波睡眠剝奪模型。SD1組每間隔24 min干擾1次,SD2組每間隔24min剝奪睡眠8 min;夜間都進(jìn)行12 h的完全睡眠剝奪。CC組模擬正常的12 h光照、12 h黑暗時間。28 d后,大鼠股動脈放血,留取睪丸組織進(jìn)行稱重,測定睪丸組織中蛋白含量、丙二醛(MDA)、谷胱甘肽過氧化物酶(GSH-Px)、超氧化物岐化酶(SOD)水平,顯微鏡下觀察睪丸病理結(jié)構(gòu)的改變。結(jié)果:SD1、SD2組大鼠的終末體質(zhì)量[(248.1±25.1)g、(232.9±10.1)g]均下降,與CC組[(268.5±1.6)g]相比差異有統(tǒng)計學(xué)意義(P均0.05);與CC組相比,SD2組睪丸相對質(zhì)量明顯升高[(54.9±3.5)×10~(-2)vs(50.0±1.3)×10~(-2),P0.05]。與CC組相比,SD2組睪丸組織蛋白含量、MDA含量、SOD活性和GSH-Px均有顯著差異[蛋白含量:(4.5±0.9)g pro/L vs 6.3±1.4)g pro/L;MDA含量:(1.3±0.3)nmol/mg pro vs(1.1±0.1)nmol/mg pro;SOD活性:(135.2±26.9)U/mg pro vs(104.3±33.1)U/mg pro;GSH-Px活性:(21.7±4.3)U/mg pro vs(15.6±4.0)U/mg pro,P均0.05],而與SD1組比較差異無統(tǒng)計學(xué)意義。SD1組睪丸病理學(xué)改變不明顯,但SD2組睪丸生精小管管腔變小,周圍間質(zhì)部分增加,間質(zhì)充血水腫明顯。結(jié)論:長期深慢波睡眠剝奪對大鼠睪丸組織結(jié)構(gòu)產(chǎn)生損傷并引起氧化應(yīng)激反應(yīng)。
[Abstract]:Aim: to study the effects of deep slow wave sleep deprivation on oxidative stress in rat testis. Methods Thirty-six five-week-old male Wistar rats were randomly divided into deep slow wave sleep deprivation group (SD1 group), deep slow wave sleep phase deprivation group (SD2 group) and control group (CC group) with 12 rats in each group. The model of deep slow wave sleep deprivation was established by using a small platform water environment. SD1 group was deprived of sleep by 24min for 8 minutes every 24 min disturbance per interval, and 12 hours of complete sleep deprivation. CC group simulated normal 12 h illumination for 12 h and darkness time of 12 h. 28 d later, SD2 group was deprived of sleep 8 minutes per interval. The rat femoral artery was bled and the testis were taken for weighing. The contents of protein, malondialdehyde (MDA), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in testis were measured. The pathological structure of testis was observed under microscope. Results compared with CC group [(268.5 鹵1.60) g], the end body weight of SD _ 2 group [(248.1 鹵25.1) g, () 232.9 鹵10.10 g] was significantly lower than that of control group (P < 0.05), and the testis relative weight of SD _ 2 group was significantly higher than that of CC group [(54.9 鹵3.5) 脳 10 ~ (-2) vs (-2) vs () 脳 10 ~ (-2) P 0.05]. Compared with CC group, SD2 group had significant difference in testicular tissue protein content, SOD activity and GSH-Px. [protein content: (4.5 鹵0.9) g / L vs 6.3 鹵1.4 g / L: (1.3 鹵0.3) nmol/mg pro vs (鹵0.1 鹵0.1 nmol/mg propron SOD activity: (135.2 鹵26.9) Umg pro vs (104.3 鹵33.1) Umg proproH-Px activity: (21.7 鹵4.3) 渭 pro vs (15.6 鹵4.0 Umg / P 0.05, but no statistical difference was found between SD1 group and SD1 group. The pathological changes of testis in SD1 group were not obvious. In SD2 group, the tubule lumen of testicular seminiferous tubules became smaller, the peripheral interstitium increased, and the interstitial hyperemia and edema were obvious. Conclusion: long term deep slow wave sleep deprivation can damage the testis and induce oxidative stress.
【作者單位】： 天津醫(yī)科大學(xué)代謝病醫(yī)院內(nèi)分泌研究所衛(wèi)生部激素與發(fā)育重點實驗室天津市代謝性疾病重點實驗室;天津醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院;天津醫(yī)科大學(xué)第二臨床醫(yī)學(xué)院;
【基金】：天津醫(yī)科大學(xué)大學(xué)生學(xué)術(shù)研究資助計劃(TMUUROP2015-08,TMUUROP2016-04);天津醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院大學(xué)生科研基金~~
【分類號】：R740

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