本文選題：B群鏈球菌 + 圍產(chǎn)期婦女��； 參考：《鄭州大學(xué)》2016年碩士論文

【摘要】：B群鏈球菌(group B streptococcus,GBS)是一類寄生于人類泌尿生殖道及下消化道的細(xì)菌,會(huì)導(dǎo)致孕婦流產(chǎn)、胎膜早破等臨床癥狀,此外,也能導(dǎo)致新生兒患敗血癥、肺炎、腦膜炎等疾病。準(zhǔn)確快速地檢測(cè)和鑒別孕產(chǎn)婦感染B群鏈球菌的情況具有重要的臨床意義。檢測(cè)B群鏈球菌所使用的標(biāo)本為陰道分泌物,分離檢測(cè)和鑒別用時(shí)較長(zhǎng),檢測(cè)手續(xù)繁瑣、精度低,目前臨床上急需一種準(zhǔn)確快速的檢測(cè)方法。B群鏈球菌顯色培養(yǎng)基在國(guó)際已有相關(guān)研究,但國(guó)內(nèi)尚未見(jiàn)報(bào)道。進(jìn)口產(chǎn)品價(jià)格昂貴、轉(zhuǎn)運(yùn)周期較長(zhǎng)、保存要求高,進(jìn)而影響其在國(guó)內(nèi)的普遍推廣和使用。本論文采用特異性酶底物顯色技術(shù),研制一種B群鏈球菌臨床檢測(cè)方法,以期獲得快速準(zhǔn)確的檢測(cè)結(jié)果。研制方法和步驟主要為:以營(yíng)養(yǎng)瓊脂培養(yǎng)基為基礎(chǔ),后通過(guò)補(bǔ)加營(yíng)養(yǎng)物質(zhì)和生長(zhǎng)因子,以靈敏度和特異性標(biāo)準(zhǔn)菌株的生長(zhǎng)情況為指標(biāo),篩選出培養(yǎng)基的基礎(chǔ)配方,再結(jié)合特異性酶顯色底物等,通過(guò)一系列優(yōu)化試驗(yàn)和正交試驗(yàn),篩選出B群鏈球菌顯色培養(yǎng)基的最佳配方;最后分別用20株臨床分離的B群鏈球菌和200份臨床疑似標(biāo)本,以科馬嘉B群鏈球菌顯色培養(yǎng)基為對(duì)照,評(píng)價(jià)該配方培養(yǎng)基的性能。結(jié)果表明:1)通過(guò)對(duì)基礎(chǔ)培養(yǎng)基和顯色培養(yǎng)基的研制和優(yōu)化,確定B群鏈球菌顯色培養(yǎng)基的主要成分,即瓊脂粉、蛋白胨、酵母浸粉、牛心浸粉、海藻糖、特異性酶顯色底物、多粘菌素B、兩性霉素B、慶大酶素、吐溫80、氯化高鐵血紅素等,p H值為7.5;2)采用臨床分離的B群鏈球菌菌株,與科馬嘉顯色培養(yǎng)基對(duì)比,二者檢出率均為100%;3)在對(duì)臨床疑似標(biāo)本檢測(cè)中,本品性能優(yōu)于科馬嘉顯色培養(yǎng)基檢測(cè)方法,科馬嘉的檢測(cè)靈敏度為85.8%,特異性為92.3%,而本品的兩項(xiàng)指標(biāo)均為100%。Vitek2 System鑒定系統(tǒng)檢測(cè)結(jié)果支持本品的結(jié)論。本論文采用特異性酶顯色底物技術(shù),研制的B群鏈球菌臨床檢測(cè)方法可獲得快速準(zhǔn)確的檢測(cè)結(jié)果,且性能優(yōu)于法國(guó)科馬嘉的同類產(chǎn)品,可用于臨床檢測(cè)。
[Abstract]:Group B Streptococcus (group B Streptococcus (GBS)) is a class of bacteria parasitic on the human urogenital tract and the lower digestive tract. It can cause pregnant women to abort, premature rupture of membranes and other clinical symptoms, in addition, it can also cause neonatal sepsis, pneumonia, meningitis and other diseases. Accurate and rapid detection and identification of pregnant and lying in and lying in women with group B Streptococcus are heavy. Clinical significance. Detection of group B Streptococcus specimens for vaginal secretions, separation detection and identification used longer, tedious procedures, low accuracy, the clinical need for an accurate and rapid detection method.B group streptococcus color culture medium has been related research in the world, but there is no report at home. The price of imported products is expensive, In this paper, a specific enzyme substrate chromogenic technique is used to develop a clinical detection method of group B Streptococcus, in order to obtain rapid and accurate detection results. The methods and steps are mainly based on the nutrient agar medium and then by supplementing camp. Material and growth factors were used to screen out the basic formula of the medium and the specific enzyme chromogenic substrate. The best formula of group B streptococcal color culture medium was screened out by a series of optimization tests and orthogonal experiments. Finally, 20 B group streptococci were used to separate the clinical isolates of Streptococcus. The bacteria and 200 clinically suspected specimens were compared with the chromogenic medium of group B Streptococcus. The results showed that: 1) the main components of the color culture medium of group B Streptococcus, namely, agar powder, peptone, yeast extract, cow heart dipping powder, trehalose, were determined by the development and optimization of the basic culture medium and color culture medium. The specific enzyme chromogenic substrate, polymyxin B, amphotericin B, Qing Da enzyme, Twain 80, high hemin chlorination, P H value 7.5; 2) the clinical isolation of group B Streptococcus, compared with the Colma coloured culture medium of the Colma color culture, the two detection rates were 100%; 3) in the detection of the suspected specimen of the bed, the quality of this product was superior to the Colma coloration culture basis. The sensitivity of the test was 85.8% and the specificity was 92.3%. The two indexes of this product were all the results of the 100%.Vitek2 System identification system. This paper uses the specific enzyme chromogenic substrate technology to develop the clinical detection method of group B Streptococcus, and the performance is better than the method. Congener's similar products can be used for clinical testing.

【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R446.5

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 張淑紅;鄭揚(yáng)云;吳清平;徐曉可;張菊梅;郭偉鵬;;兩種大腸桿菌顯色培養(yǎng)基檢測(cè)效果的比較[J];食品研究與開(kāi)發(fā);2012年10期

2 張青,汪定成,劉樹(shù)林;顯色培養(yǎng)基用于陰道炎霉菌的檢測(cè)[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2002年04期

3 龔云偉;王平;董偉力;張文超;孔祥云;;應(yīng)用科碼嘉顯色培養(yǎng)基快速分離病原微生物[J];中國(guó)衛(wèi)生工程學(xué);2007年06期

4 盧勉飛;蔡芷荷;吳清平;何天文;;顯色培養(yǎng)基快速檢測(cè)食品中大腸桿菌的應(yīng)用研究[J];中國(guó)熱帶醫(yī)學(xué);2009年07期

5 胡楊峰;何艷玲;劉沛;;自主研制志賀氏菌顯色培養(yǎng)基的應(yīng)用效果評(píng)價(jià)[J];中國(guó)國(guó)境衛(wèi)生檢疫雜志;2012年01期

6 陳榮;羅燕萍;葉麗艷;王歡;王磊利;周光;;應(yīng)用顯色培養(yǎng)基快速檢測(cè)血培養(yǎng)中耐甲氧西林金黃色葡萄球菌[J];中華醫(yī)院感染學(xué)雜志;2012年13期

7 王靜;陳萍;李倩倩;任常菲;;產(chǎn)氣莢膜梭菌選擇性顯色培養(yǎng)基的研制[J];衛(wèi)生研究;2013年04期

8 黃汝添;吳清平;張菊梅;郭偉鵬;吳許文;;三種金黃色葡萄球菌顯色培養(yǎng)基檢測(cè)效果的比較[J];微生物學(xué)通報(bào);2009年08期

9 汪定成,張青,劉茂賢,劉樹(shù)林;顯色培養(yǎng)基在臨床快速檢測(cè)念珠菌的應(yīng)用[J];中華醫(yī)學(xué)檢驗(yàn)雜志;1999年06期

10 丁宸;顯色培養(yǎng)基在臨床鑒定酵母菌中的應(yīng)用[J];醫(yī)藥論壇雜志;2004年04期

相關(guān)會(huì)議論文 前4條

1 蔡芷荷;盧勉飛;葉青華;黃嶺芳;吳清平;;念珠菌顯色培養(yǎng)基的應(yīng)用研究[A];2012年鄂粵微生物學(xué)學(xué)術(shù)年會(huì)——湖北省暨武漢微生物學(xué)會(huì)成立六十年慶祝大會(huì)論文集[C];2012年

2 時(shí)威;騰昆侖;盧勉飛;蔡芷荷;吳清平;;金黃色葡萄球菌顯色培養(yǎng)基檢測(cè)效果初步評(píng)價(jià)[A];第五屆全國(guó)微生物資源學(xué)術(shù)暨國(guó)家微生物資源平臺(tái)運(yùn)行服務(wù)研討會(huì)論文摘要集[C];2013年

3 盧勉飛;蔡芷荷;吳清平;葉青華;;顯色培養(yǎng)基在檢測(cè)蠟樣芽孢桿菌中的應(yīng)用研究[A];2010第二屆中國(guó)食品安全高峰論壇論文集[C];2010年

4 王毅謙;吳福平;邵景東;陳君義;張莉軍;;顯色培養(yǎng)基在檢測(cè)食品中志賀氏菌的應(yīng)用研究[A];中國(guó)食品科學(xué)技術(shù)學(xué)會(huì)第七屆年會(huì)論文摘要集[C];2010年

相關(guān)碩士學(xué)位論文 前1條

1 楊詠康;一種B群鏈球菌臨床檢測(cè)方法的研制[D];鄭州大學(xué);2016年

，

本文編號(hào)：1857242