本文選題：端粒 + 放射敏感性　； 參考：《廣東醫(yī)學》2017年22期

【摘要】：目的探討不同腫瘤細胞內源性DNA雙鏈斷裂(DSBs)水平與放射敏感性參數SF2是否存在相關性。方法選取肺癌細胞系H1299、A549,乳腺癌細胞系MCF-7、MDA-MB-435s為研究對象。利用2 Gy射線照射后克隆形成實驗檢測不同細胞的放射敏感性指標SF2;通過實時熒光定量PCR方法測定不同細胞相對端粒長度變化;通過免疫熒光標記法標記不同腫瘤細胞γ-H2AX蛋白,檢測不同細胞內源性DSBs損傷水平。通過統(tǒng)計學分析評價腫瘤細胞內源性DSBs與放射敏感性的關系。結果免疫熒光法標記γ-H2AX蛋白顯示4種腫瘤細胞內源性DSBs水平腫瘤細胞除H1299與A549之間差異無統(tǒng)計學意義(P0.05)外,其他任意兩組之間差異均有統(tǒng)計學意義(P0.05);4種腫瘤細胞的內源性DSBs水平與SF2、端粒長度的相關系數R分別為-0.95和-0.97,有統(tǒng)計學意義(P0.05)。結論腫瘤細胞內源性DSBs可以作為一種預測腫瘤細胞放射敏感性的標志,它們之間的相關性可能與端粒部分參與了內源性DSBs產生和修復有關。
[Abstract]:Objective to investigate the correlation between the levels of endogenous DNA double strand breaks (DSBs) and radiosensitivity parameters (SF2) in different tumor cells. Methods Lung cancer cell line H1299 A549 and breast cancer cell line MCF-7 MDA-MB-435s were selected. The radiosensitivity index SF _ 2 of different cells was detected by cloning assay after irradiation of 2 Gy, the relative telomere length of different cells was measured by real-time fluorescence quantitative PCR, and 緯 -H2AX protein was labeled by immunofluorescence labeling method. The levels of endogenous DSBs damage in different cells were detected. The relationship between endogenous DSBs and radiosensitivity of tumor cells was evaluated by statistical analysis. Results Immunofluorescence labeling of 緯 -H2AX protein showed that there was no significant difference between H1299 and A549 in the endogenous DSBs level of four kinds of tumor cells except H1299 and A549 (P 0.05). There were significant differences between other two groups in the level of endogenous DSBs and SF _ 2. The correlation coefficients of telomere length were -0.95 and -0.97, respectively. Conclusion Endogenous DSBs of tumor cells can be used as a marker to predict radiosensitivity of tumor cells. The correlation between them may be related to the part of telomere involved in the production and repair of endogenous DSBs.
【作者單位】： 許昌學院醫(yī)學院;許昌市中心醫(yī)院腫瘤科;鄭州大學附屬腫瘤醫(yī)院超聲科;
【基金】：2014年河南省第三批科技計劃項目(編號:142300410382)
【分類號】：R730.55

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