本文選題：氧化損傷 + 聚合人胎盤(pán)血紅蛋白��； 參考：《中國(guó)輸血雜志》2017年04期

【摘要】：目的探討輸注不同濃度聚合人胎盤(pán)血紅蛋白(Poly PHb)對(duì)大鼠造成的氧化損傷。方法建立動(dòng)物換血模型,將50只SD大鼠隨機(jī)均分為假手術(shù)組:僅做股動(dòng)/靜脈插管處理;氯化鈉注射液(對(duì)照)組:股動(dòng)/靜脈插管,輸注氯化鈉注射液行換血實(shí)驗(yàn);2%、4%及6%Poly PHb 3個(gè)實(shí)驗(yàn)組:股動(dòng)/靜脈插管,分別輸注相應(yīng)濃度的Poly PHb行換血實(shí)驗(yàn)。分別在大鼠換血前、換血70%后即刻觀測(cè)平均動(dòng)脈壓(MAP),換血完成后即刻從大鼠股動(dòng)脈取血0.5 m L/只,做動(dòng)脈血血?dú)夥治?并檢測(cè)各組大鼠血漿中還原型谷胱甘肽(GSH)濃度、超氧化物歧化酶(SOD)活力、總抗氧化能力(T-AOC)、丙二醛(MDA)濃度、8-羥基脫氧鳥(niǎo)苷(8-OHDG)濃度。結(jié)果 1)大鼠換血70%后,2%、4%及6%Poly PHb組與對(duì)照組相比,GSH(μmol/L)分別為33.76±14.33、47.98±21.88、39.73±15.54及10.25±5.70(P0.01);SOD(U/m L)分別為238.07±27.39、355.73±35.42、405.26±45.01及138.46±57.16(P0.01);T-AOC(U/m L)分別為20.50±7.87、32.65±4.38、38.03±4.50及5.40±2.22(P0.01)。2)大鼠換血70%后,2%、4%及6%Poly PHb組與對(duì)照組相比,MDA濃度(nmol/m L)隨著Poly PHb濃度的增高而增高:45.08±4.12、54.39±3.16、58.52±6.33及2.85±0.59(P0.01)。3)大鼠換血70%后,2%、4%及6%Poly PHb組與對(duì)照組相比,大鼠血漿8-OHDG濃度(nmol/m L)未發(fā)生明顯變化:2.54!0.90、2.76!0.99、2.79!0.82及2.26!0.42(P0.05)。結(jié)論 Poly PHb對(duì)大鼠造成脂質(zhì)過(guò)氧化損傷,且氧化損傷程度隨著Poly PHb濃度的增高而加重,但并未對(duì)大鼠DNA造成損傷。
[Abstract]:Objective to investigate the oxidative damage induced by different concentrations of polymerized human placental hemoglobin (Poly PHb) in rats.Methods 50 SD rats were randomly divided into sham-operation group (femoral artery / vein intubation only) and sodium chloride injection (control) group (femoral / venous intubation).Three experimental groups: femoral artery / vein catheterization, transfusion of sodium chloride injection with 4% and 6%Poly PHb, and transfusion of corresponding concentration of Poly PHb were performed.The mean arterial pressure (MAPH) was measured immediately before and 70% after the exchange of blood in rats, and 0.5 mL of blood was taken from the femoral artery of rats immediately after the exchange of blood. The arterial blood gas was analyzed and the plasma GSH concentration of reduced glutathione was measured in each group.Superoxide dismutase (SOD) activity, total antioxidant capacity (T-AOC), malondialdehyde (MDA) concentration, 8-hydroxydeoxyguanosine (8-OHDG) concentration.緇撴灉 1)澶ч紶鎹㈣70%鍚，

本文編號(hào)：1739014