本文選題：鮑曼不動桿菌　切入點：碳青霉烯耐藥　出處：《中國抗生素雜志》2017年08期

【摘要】：目的碳青霉烯耐藥鮑曼不動桿菌(CRAB)逐年升高的分離率和全球播散已成為極為嚴(yán)重的問題,本研究目的是闡明我院CRAB的耐藥機(jī)制和分子流行病學(xué)特征。方法使用gyrB多重PCR方法將我院2014年7月—2015年6月分離的327株非重復(fù)醋酸鈣-鮑曼不動桿菌復(fù)合體(ABC)鑒定到種,VITEK-2儀器法和E-test法測定抗菌藥物的最低抑菌濃度(minimal inhibitory concentration MIC),PCR方法檢測常見的碳青霉烯酶耐藥基因、ISAba1并測序,Turton 2組多重PCR方法進(jìn)行分子分型。結(jié)果327株ABC中有315株鮑曼不動桿菌(ABA)、9株皮特不動桿菌和3株醫(yī)院不動桿菌,ABA、皮特和醫(yī)院不動桿菌對亞胺培南的耐藥率分別為91.7%、0和66.7%,耐藥株主要分離自急診科和呼吸ICU。ABA bla_(OXA-23-like)和ISAba1檢出率均為91.1%,測序均為bla_(OXA-23),bla_(OXA-51-like)和ISAba1檢出率分別為100%和0.3%,測序主要為bla_(OXA-66)和bla_(OXA-69),未檢測到其他OXAs型、NDM、IMP、GIM、KPC、SIM、VIM和GES酶基因。Turton分子分型76.8%屬于國際克�、蛐�(IC Ⅱ)。結(jié)論攜帶bla_(OXA-23)和ISAba1的IC Ⅱ克隆是我院主要的流行克隆株,克隆播散是CRAB感染增加和暴發(fā)的重要原因。
[Abstract]:Objective the increasing isolation rate and global spread of carbapene-resistant Acinetobacter baumannii have become a serious problem. The aim of this study was to elucidate the mechanism of drug resistance and molecular epidemiological characteristics of CRAB in our hospital. Methods 327 strains of acinetobacter Acinetobacter Acinetobacter Acinetobacter Acetate isolated from July 2014 to. VITEK-2 instrument method and E-test method were used to determine the minimum inhibitory concentration minimal inhibitory concentration (MEC) of antimicrobial agents. Results the molecular typing was carried out by multiplex PCR method for the detection of the common carbapenem resistance gene (ISAba1) and sequencing Turton 2 groups. Results 31515 out of 327 ABC strains were detected by multiplex PCR. Acinetobacter baumannii 9 strains of Acinetobacter Pitt and 3 strains of Acinetobacter nosocomial ABA, Pitt and Acinetobacter nosocomial strains were resistant to imipenem 91.7% and 66.7% respectively. The resistant strains were mainly isolated from emergency department and respiratory ICU.ABA blastoid like (OXA-23-like) and ISAba1 detection rate. All of them were 91.1, and the detection rates of ISAba1 and ISAba1 were 100% and 0.3%, respectively, and the detection rates of OXA-51-like and ISAba1 were 100% and 0.3%, respectively, and the sequence was mainly OXA-66) and OXA-690.Conclusion No other OXAs type NDMPIMPIMPIMPIMGIMVIM and GES gene (.Turton) were detected. Conclusion\\\;\\\;\\\%)\% of the IC- 鈪，

本文編號：1678274