本文關鍵詞： 鮑曼不動桿菌 替加環(huán)素 誘導耐藥 適應度代價　出處：《四川大學學報(醫(yī)學版)》2017年02期 　論文類型：期刊論文

【摘要】：目的了解長期抗菌藥物壓力及常用紫外線消毒對鮑曼不動桿菌耐藥性的影響及替加環(huán)素(TGC)耐藥鮑曼不動桿菌耐藥性在群體中存續(xù)的能力。方法選取臨床分離TGC敏感鮑曼不動桿菌3株,分別采用多步法及紫外線照射體外誘導耐藥,微量肉湯稀釋法檢測誘導前后鮑曼不動桿菌對TGC的最低抑菌濃度(MIC)變化。選取臨床分離TGC耐藥鮑曼不動桿菌2株,在空白MHA平板中反復傳代,測定其MIC值。最后選取TGC敏感株及TGC誘導同源耐藥株進行TGC耐藥株適應度代價研究。結果應用多步法成功誘導出TGC耐藥株;紫外線照射雖然對TGC耐藥性未發(fā)生影響,但其MIC值有上升趨勢。TGC耐藥鮑曼不動桿菌傳代40代后菌株MIC值無明顯變化。TGC敏感株及TGC誘導同源耐藥株進行相同條件下培養(yǎng),體外單獨培養(yǎng)時耐藥株達到對數(shù)生長期及平臺期的時間均較敏感株長;混合培養(yǎng)時,隨著連續(xù)傳代,耐藥株菌落數(shù)量較敏感株迅速減少,甚至耐藥株被清除。結論 TGC可誘導鮑曼不動桿菌產生獲得性耐藥。鮑曼不動桿菌對TGC的耐藥可能具有遺傳穩(wěn)定性。TGC耐藥株較敏感株適應性下降。
[Abstract]:Objective to investigate the effect of long-term antimicrobial pressure and ultraviolet disinfection on drug resistance of Acinetobacter baumannii and TGC of tegacycline. The ability of drug resistance of Acinetobacter baumannii to survive in the population. Methods three strains of Acinetobacter baumannii sensitive to TGC were selected. Multistep method and ultraviolet radiation were used to induce drug resistance in vitro. The minimal inhibitory concentration (MIC) of Acinetobacter baumannii to TGC before and after induction was detected by broth dilution method. Two strains of TGC resistant Acinetobacter baumannii were selected. Repeated passage in blank MHA plate. Finally, TGC sensitive strains and TGC induced homologous drug resistant strains were selected to study the cost of TGC resistance. Results the multistep method was used to induce TGC resistant strains successfully. Ultraviolet irradiation had no effect on TGC resistance. However, the MIC value of Acinetobacter baumannii showed an increasing trend. The MIC value of Acinetobacter baumannii strains did not change significantly after 40 generations. TGC-sensitive strains and TGC induced homologous resistant strains were cultured under the same conditions. The time between logarithmic growth stage and plateau stage of drug-resistant strain was longer than that of sensitive strain in vitro. When mixed culture, with continuous passage, the number of drug-resistant strains was decreased rapidly than that of sensitive strains. Even the resistant strain was cleared. Conclusion. TGC could induce acquired drug resistance of Acinetobacter baumannii, and the resistance of Acinetobacter baumannii to TGC may have genetic stability.
【作者單位】： 四川大學華西醫(yī)院感染性疾病中心;
【基金】：科技部國家科技支撐計劃課題(No.2012EP001000)資助
【分類號】：R446.5
【正文快照】： 鮑曼不動桿菌是院內感染的重要條件致病菌,具有強大的獲得耐藥性和克隆傳播的能力,多重耐藥(MDR)、泛耐藥(XDR)鮑曼不動桿菌呈世界性流行[1],在免疫功能低下患者中可引起嚴重致死性感染。雖然替加環(huán)素(TGC)對不動桿菌感染療效有限[2],但除多黏菌素外,TGC仍是目前治療MDR/XDR鮑

【參考文獻】

相關期刊論文 前3條

1 王燕;周W，

本文編號：1473512