本文選題：細胞移植 + 移植　； 參考：《中國臨床康復》2005年46期

【摘要】：目的:在過去10年中,自體軟骨細胞移植技術成為治療關節(jié)軟骨缺損的最佳方案。回顧自體軟骨細胞移植技術的發(fā)展過程并對其未來方向作以簡單設想。資料來源:應用計算機檢索Medline1957-01/2005-09的文章,檢索詞為“autologous/autogeneouschondrocyteimplantation/transplantation,car-tilagedefect,ACI”,限定文章語言種類為English;同時計算機檢索相關期刊論文1994-01/2005-09期間的相關文章,檢索詞“骨性關節(jié)炎、關節(jié)軟骨剝脫、關節(jié)軟骨缺損、關節(jié)軟骨損傷、軟骨細胞移植、自體軟骨細胞移植”,限定文章語言種類為中文。另外在google搜索引擎及有關生物醫(yī)學公司網(wǎng)站搜集相關資料。資料選擇:納入標準:①軟骨細胞體外培養(yǎng)及移植的基礎理論。②動物實驗。③FDA審批文件。④臨床應用報告。⑤患者隨訪分析。⑥自體軟骨細胞移植技術與其他治療方法療效對比。排除標準:①較陳舊的文獻。②重復研究。資料提煉:共收集到300余篇(頁)與關節(jié)軟骨損傷、缺損及其治療相關的文獻及網(wǎng)頁,其中56篇(頁)符合納入標準,排除的240篇(頁)文章系同一類重復性研究和近似的網(wǎng)絡文章。資料綜合:①軟骨細胞的體外大規(guī)模培養(yǎng)技術和在動物體進行的軟骨細胞移植實驗為自體軟骨細胞移植在人體的進一步研究奠定了理論基礎。②初期的自體軟骨細胞移植技術通過縫合自體骨膜來制造封閉關節(jié)軟骨缺損病灶的空腔,再將體外培養(yǎng)擴增后的自體軟骨細胞混懸液注射進入該空腔從而使細胞在病灶處形成新生軟骨。③后發(fā)展為膠原膜被用來替代自體骨膜作為自體軟骨細胞移植術中的病灶封閉材料。④近年來又研制出將體外培養(yǎng)擴增后的自體軟骨細胞與Ⅰ/Ⅲ型膠原膜復合制成固態(tài)膜片,再將該膜用生物膠貼附在關節(jié)軟骨缺損部位,移植的細胞以膠原膜為基質(zhì)而增生,修復原軟骨缺損。⑤基質(zhì)誘導的自體軟骨細胞移植技術還不是最完美的方案,仍然存在不足。因此需要向更安全、有效,更易于運輸、保存及更低制造成本等方向研究。結論:經(jīng)過10多年的不斷改進,自體軟骨細胞移植技術已經(jīng)成為目前治療關節(jié)軟骨缺損的最佳方案,其新一代的基質(zhì)誘導的自體軟骨細胞移植技術應該得到臨床普及來治療這一疾病。
[Abstract]:Objective: in the past 10 years, autologous chondrocyte transplantation has been the best method for the treatment of articular cartilage defects. This paper reviews the development of autologous chondrocyte transplantation and gives a simple idea of the future direction of autologous chondrocyte transplantation. Data sources: Medline1957-01/2005-09 articles were searched by computer with the key words "autologous-autogeneouschondrocyteimplantationr transplanationr transplantationcar-tilage defectl ACI", and the Chinese Journal Full-text Database was searched by computer for articles related to the period 1994-01 / 2005-09, with the key words "osteoarthritis, articular cartilage exfoliation," Articular cartilage defect, articular cartilage injury, chondrocyte transplantation, autologous chondrocyte transplantation. In addition in the google search engine and related biomedical company website to collect relevant information. Data selection: basic theory of in vitro culture and transplantation of chondrocytes from standard 1: .2 Animal experiment .3FDA approval document .4 Clinical application report .5 patients follow up analysis of 6 autologous chondrocyte transplantation technique and its application. His treatment was compared. Exclusion Standard: 1 older document .2 duplicate study. Data extraction: more than 300 articles (pages) related to articular cartilage injury, defects and their treatment were collected, 56 of which met the inclusion criteria. The 240 excluded articles were the same type of repetitive research and similar online articles. Data synthesis of mass culture of chondrocytes in vitro and chondrocyte transplantation in animals laid a theoretical foundation for further study of autologous chondrocyte transplantation in human body. 2. Early autologous chondrocytes By suturing autologous periosteum, the technique of cell transplantation is used to seal the cavity of the articular cartilage defect. And then the suspension of autologous chondrocytes cultured in vitro was injected into the cavity, so that the cells formed new cartilage at the focus, and then developed into collagen membrane instead of autologous periosteum as autologous chondrocyte transplantation. Recently, autologous chondrocytes cultured in vitro were combined with collagen 鈪，

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