本文選題：近平滑假絲酵母 + 分離鑒定�。� 參考：《中國(guó)糧油學(xué)報(bào)》2017年10期

【摘要】：為了制備生物柴油而獲得高產(chǎn)油脂的酵母,分離出1株產(chǎn)油酵母,利用分子生物學(xué)技術(shù)對(duì)其RNA基因內(nèi)轉(zhuǎn)錄區(qū)(ITS區(qū))進(jìn)行了克隆測(cè)序,并與Gen Bank中已有的菌株基因序列進(jìn)行比對(duì);同時(shí),對(duì)該菌株生長(zhǎng)發(fā)育和油脂合成進(jìn)行了研究;對(duì)生物量、糖氮代謝、油脂含量以及脂肪酸組分進(jìn)行了檢測(cè)分析。結(jié)果表明:該菌株ITS序列長(zhǎng)度為479 bp,與Gen Bank中近平滑假絲酵母相比較同源率接近100%,形態(tài)結(jié)果和分子生物學(xué)鑒定表明該菌株為近平滑假絲酵母;該菌在0~12 h期間菌體數(shù)量穩(wěn)定增長(zhǎng),糖氮消耗明顯為近平滑假絲酵母的發(fā)酵適應(yīng)期;12~48 h,此時(shí)菌種快速繁殖,糖氮消耗非常顯著,為近平滑假絲酵母的對(duì)數(shù)期;48~120 h,菌體數(shù)量保持穩(wěn)定基本不再增長(zhǎng),糖氮消耗穩(wěn)定為近平滑假絲酵母的穩(wěn)定期;120 h以后,菌體數(shù)量減少,糖氮基本消耗完畢為近平滑假絲酵母的衰退期;在120 h左右胞內(nèi)油脂積累達(dá)到最大值,油脂產(chǎn)量為4.5g/L,含油率約為22%,脂肪酸組成主要以油酸為主,并含有少量棕櫚酸和亞油酸。因此,該菌作為油脂菌株生產(chǎn)生物柴油具有一定前景,為以后調(diào)整培養(yǎng)基含量來增加菌種油脂產(chǎn)量提供了參考。
[Abstract]:In order to obtain high oil production yeast from biodiesel, one oil producing yeast was isolated and cloned and sequenced by molecular biology technique. The sequence of its region of RNA gene was cloned and compared with that of the existing strain in Gen Bank. At the same time, the growth and development of the strain and the synthesis of oil were studied, and the biomass, sugar and nitrogen metabolism, oil content and fatty acid composition were detected and analyzed. The results showed that the length of the ITS sequence of the strain was 479 BP, and the homology rate of the strain was close to 100. The morphological results and molecular biological identification showed that the strain was nearly Candida smoothing. During the period of 0 ~ 12 h, the number of the bacteria increased steadily, and the consumption of sugar and nitrogen was 1248 h during the fermentation adaptation period of Candida smoothing. At this time, the strain rapidly propagated, and the consumption of sugar and nitrogen was very significant. In the logarithmic phase of Candida smoothing for 120 h, the number of mycelia remained stable and no longer increased, and the consumption of sugar and nitrogen was stable to the stable period of nearly Candida smoothing for 120 h, and the number of mycelia decreased after 120 h. The basic consumption of sugar and nitrogen was near decaying period of Candida smoothing, the accumulation of lipid reached the maximum at about 120 h, the oil yield was 4.5 g / L, the oil content was about 22, and the fatty acid composition was mainly oleic acid and a little palmitic acid and linoleic acid. Therefore, it has a certain prospect to produce biodiesel as oil strain, which provides a reference for adjusting the content of culture medium to increase the yield of oil.
【作者單位】： 武漢輕工大學(xué)食品科學(xué)與工程學(xué)院;湖北天基生物能源發(fā)展有限公司;中國(guó)科學(xué)院武漢病毒研究所;
【分類號(hào)】：TE667
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本文編號(hào)：1952720