本文關(guān)鍵詞：黃芩苷在黃芩植株體內(nèi)積累規(guī)律的研究　出處：《山東中醫(yī)藥大學(xué)》2004年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 黃芩 黃芩苷 外界因素 POD酶 蛋白質(zhì)

【摘要】：為了解黃芩植株體內(nèi)黃芩苷的合成積累規(guī)律及其影響因素，達(dá)到控制或提高栽培藥材質(zhì)量的目的，我們采用種子發(fā)芽與盆栽方式，利用高效毛細(xì)管電泳、高效液相色譜等手段，研究了黃芩苷在黃芩植株各器官中的分布及生長(zhǎng)時(shí)間、溫度、光照、營(yíng)養(yǎng)元素等因素對(duì)黃芩苷含量的影響。結(jié)果表明：黃芩種子不含黃芩苷，植株其它器官中均含有，但含量多少依次為：根花葉莖，主根側(cè)根支根，韌皮部木質(zhì)部；種子萌發(fā)至子葉展開(kāi)時(shí)，黃芩苷才開(kāi)始產(chǎn)生，隨著幼苗不斷長(zhǎng)大含量逐步提高，一年生植株的生長(zhǎng)時(shí)間越長(zhǎng)，根中黃芩苷的含量越高：光照具有促進(jìn)黃芩苷合成的作用，光照條件下生長(zhǎng)幼苗中的黃芩苷含量顯著高于黑暗條件下生長(zhǎng)者，光照越強(qiáng)，植株根部的黃芩苷含量越高；溫度對(duì)黃芩苷的合成也有影響，以25℃最適。補(bǔ)充營(yíng)養(yǎng)元素能促進(jìn)黃芩苷合成，在種子發(fā)芽時(shí)以鉀肥和氮磷復(fù)合肥的促進(jìn)作用最大，對(duì)砂培植株來(lái)講，銅有抑制作用，，而磷、鉀等元素有促進(jìn)作用；在植株?duì)I養(yǎng)狀況較差的情況下，補(bǔ)充蔗糖與葡萄糖能夠提高黃芩苷含量，正常植株補(bǔ)充蔗糖與葡萄糖時(shí)，黃芩苷的含量有所降低；黃芩苷含量與可溶性蛋白質(zhì)含量之間具有一定的相關(guān)性，在種子萌發(fā)幼苗生長(zhǎng)期間為明顯的負(fù)相關(guān)性，在施肥補(bǔ)充營(yíng)養(yǎng)元素后為明顯的正相關(guān)性：各器官均有過(guò)氧化物酶存在，但POD酶譜中的帶1，只存在于根中，在莖、葉及花中存在較少或不存在，既使在根中也會(huì)因生長(zhǎng)時(shí)間不同而存在譜帶寬度的差異；不同產(chǎn)地種子的蛋白電泳圖譜有明顯差異，證明其遺傳性發(fā)生了變化。
[Abstract]:In order to understand the synthesis and accumulation of baicalin in Scutellaria baicalensis plant and its influencing factors, and to control or improve the quality of cultivated medicinal materials, we adopted seed germination and pot culture, and used high performance capillary electrophoresis (HPCE). The distribution, growth time, temperature and illumination of baicalin in various organs of Scutellaria baicalensis were studied by high performance liquid chromatography (HPLC). The results showed that baicalin was not contained in the seeds of Scutellaria baicalensis, but was found in other organs of the plant, but the contents were in order as follows: root, flower, leaf stem, main root, lateral root branch and root. Phloem xylem; Baicalin began to produce only when the seeds germinated to cotyledon development, and the growing time of annual plants increased with the increasing of seedling growth. The higher the content of baicalin in the root: light can promote the synthesis of baicalin, the higher the content of baicalin in the growth seedlings was, the stronger the light was. The higher the content of baicalin in the root; Temperature also had an effect on the synthesis of baicalin, and the optimum temperature was 25 鈩

本文編號(hào)：1362760