鄱陽(yáng)湖濕地剖面土壤微生物群落結(jié)構(gòu)及功能的變化特征
發(fā)布時(shí)間：2019-06-11 20:10
【摘要】：鄱陽(yáng)湖濕地是我國(guó)最大的天然淡水湖泊濕地,季節(jié)性水位動(dòng)態(tài)明顯,微地形復(fù)雜多變,擁有豐富的自然資源,隨著人們對(duì)濕地功能性認(rèn)識(shí)的不斷高,鄱陽(yáng)湖區(qū)濕地生態(tài)系統(tǒng)研究近年來(lái)受到學(xué)者們的普遍關(guān)注。對(duì)濕地土壤剖面的研究示,深層土壤中可能蘊(yùn)含有大量的微生物資源,對(duì)濕地生態(tài)系統(tǒng)的物質(zhì)轉(zhuǎn)化和能量循環(huán)具有重要影響。然而對(duì)于微生物群落的結(jié)構(gòu)組成、功能、代謝活性如何隨著深度剖面分布,主要受何種因素影響,我們知之甚少且缺乏系統(tǒng)研究。因此,本研究以鄱陽(yáng)湖濕地土壤為研究對(duì)象,樣地選擇在蚌湖(BH)、常湖池(CHC)、南磯山(東湖)(NJS)等三個(gè)典型區(qū),取樣土壤剖面深度為1m,分為5層(0-20cm、20-40cm、40-60cm、60-80cm、80-100cm)。采用16S rRNA高通量測(cè)序、q-PCR、Biolog-ECO等多樣研究方法,對(duì)土壤理化性質(zhì)、胞外酶活性、碳源代謝活性、微生物生物量以及細(xì)菌豐富度和群落結(jié)構(gòu)進(jìn)行分析。以闡明鄱陽(yáng)湖濕地土壤微生物群落結(jié)構(gòu)及代謝功能的空間變化,尤其是沿土壤剖面深度的變化特征,為多微生物組學(xué)角度示土壤演變機(jī)制供一條新的途徑。結(jié)果表明:(1)鄱陽(yáng)湖濕地土壤主要優(yōu)勢(shì)菌門(mén)為綠彎菌門(mén)(30.83%),其次是變形菌門(mén)(21.37%)和酸桿菌門(mén)(18.85%)。在更細(xì)的分類(lèi)層次——科水平,主要的優(yōu)勢(shì)物種是Anaerolineaceae(17.49%),其次是Acidobacteriaceae_(Subgroup_1)(9.36%)和HSB_OF53-F07(8.00%)。與此同時(shí),鄱陽(yáng)湖濕地土壤細(xì)菌豐度(1.60×108-7.49×1010 copies/g)和Chao1指數(shù)(663-2664)也高于其它淡水濕地生態(tài)系統(tǒng)。使用METAGENassist進(jìn)行基于細(xì)菌群落的代謝功能預(yù)測(cè),結(jié)果顯示:表層(0-20cm)土壤中細(xì)菌群落的代謝功能主要包括硫酸鹽的還原(41.9%),氨氧化(35.8%),亞硝酸鹽的還原(33.3%),脫鹵(27.7%),硫化物的氧化(25.8%),木聚糖降解(20.1%),氮的固定(11.0%)等;深層(20-100cm)土壤中細(xì)菌群落的代謝功能主要包括氨氧化(37.7%),硫酸鹽的還原(34.3%),亞硝酸鹽的還原(27.8%),硫化物的氧化(25.7%),脫鹵(22.9%),甲烷氧化(20.0%),木聚糖降解(18.7%)。(2)鄱陽(yáng)湖濕地土壤細(xì)菌豐度及群落結(jié)構(gòu)沿土壤剖面深度顯著改變,且差異性主要表現(xiàn)在表層(0-20cm)土壤和深層(20-100cm)土壤之間,其中綠彎菌門(mén)、變形菌門(mén)以及酸桿菌門(mén)在濕地土壤剖面深度上有顯著的差異性。細(xì)菌群落代謝功能方面,反硝化功能(7.0%)和儲(chǔ)存聚羥基丁酸酯的功能(0.02%)只存在于表層土壤,而萘降解(0.9%)和硒酸鹽還原劑功能(0.04%)只存在于深層土壤中。值得注意的是,深層土壤中的甲烷氧化功能(20.00%)明顯的高于表層土壤(0.08%)。進(jìn)一步研究發(fā)現(xiàn),盡管在不同的樣地間,土壤細(xì)菌群落結(jié)構(gòu)存在顯著的差異,但差異性遠(yuǎn)不及深度上的差異顯著。(3)鄱陽(yáng)湖濕地土壤微生物功能特性(微生物生物量、胞外酶活性、碳源代謝功能)顯著受到土壤深度的影響,表層(0-20cm)土壤中微生物活性最高,土壤深度達(dá)到60cm以后,微生物活性開(kāi)始穩(wěn)定,但深層土壤中仍然進(jìn)行著由微生物參與的復(fù)雜代謝活動(dòng)。此外,僅微生物碳源代謝活性(AWCD)在樣地間存在差異,土壤微生物生物量、胞外酶活性不隨樣地而發(fā)生改變,表明土壤微生物作為環(huán)境變化的敏感指標(biāo),其群落結(jié)構(gòu)的改變會(huì)優(yōu)先于某些代謝功能的改變,以此來(lái)應(yīng)對(duì)外界環(huán)境的變化。(4)通過(guò)相關(guān)性分析以及冗余分析(RDA)發(fā)現(xiàn),鄱陽(yáng)湖濕地土壤微生物功能特性和細(xì)菌群落結(jié)構(gòu)組成主要受到土壤有機(jī)質(zhì)(AFDM)及營(yíng)養(yǎng)元素(TOC、TN)的影響,除此之外,土壤含水量(SM)也是影響因素之一。其中AFDM、TOC和TN沿土壤剖面深度顯著減小是導(dǎo)致鄱陽(yáng)湖濕地土壤微生物的功能活性以及細(xì)菌群落結(jié)構(gòu)隨土壤剖面深度變化的主要原因;土壤SM在樣地間的差異性,則使得土壤微生物碳源代謝活性及細(xì)菌群落結(jié)構(gòu)隨樣地不同而發(fā)生改變。(5)通過(guò)相關(guān)性分析以及冗余分析發(fā)現(xiàn),Bradyrhizobiaceae、4-29、Xanthobacteraceae與β-木糖苷酶(Bxyl)、過(guò)氧化物酶(Pero)、β-葡萄糖苷酶(Bglu)和土壤磷酸酶(Phos)的活性變化存在緊密的聯(lián)系,Spirochaetaceae與乙酰氨基葡萄糖苷酶(NAG)的活性變化相關(guān)。Acidothermaceae、1921-3、HSB_OF53-F07、Cystobacteraceae、sh765B-TzT-35是影響碳源代謝活性的最主要物種。綜上所述,鄱陽(yáng)湖濕地土壤性質(zhì)、土壤細(xì)菌群落結(jié)構(gòu)、細(xì)菌豐富度和功能特性均隨著深度表現(xiàn)出顯著的差異,而在水平距離上差異并不明顯。與深層土壤(20-100cm)相比,盡管表層土壤(0-20cm)微生物的功能更為活躍,細(xì)菌豐度高,但是深層土壤(甚至到100cm深度)仍然具有復(fù)雜的細(xì)菌群落組成和較高的代謝功能活性,這一點(diǎn)與其他生態(tài)系統(tǒng)中土壤微生物的垂直分布不太一致。這就示我們?cè)跐竦厣鷳B(tài)系統(tǒng)中,不同深度的土壤微生物具有不同的群落組成和代謝功能,深層土壤的微生物在土壤演化中的作用值得關(guān)注。本研究的結(jié)果初步揭示了鄱陽(yáng)湖濕地土壤微生物的空間分布規(guī)律,深化了我們對(duì)于濕地生態(tài)系統(tǒng)的認(rèn)識(shí)。全面的、系統(tǒng)的了解濕地生態(tài)系統(tǒng),是濕地環(huán)境治理、資源保護(hù)、可持續(xù)發(fā)展的重要科學(xué)基礎(chǔ)。本項(xiàng)目特色:首次采用較先進(jìn)的研究手段,系統(tǒng)研究了鄱陽(yáng)湖濕地土壤微生物群落結(jié)構(gòu)和功能特性的空間分布,為預(yù)測(cè)鄱陽(yáng)湖濕地生態(tài)系統(tǒng)微生物的演化趨勢(shì)和功能活性供參考;較早的引入METAGENassist,將16S rRNA的高通量測(cè)序結(jié)果與群落代謝功能相聯(lián)系,幫助進(jìn)一步了解濕地土壤沿剖面變化的土壤微生物的功能多樣性和重要性。
[Abstract]:Poyang Lake wetland is the largest natural freshwater lake wetland in China, the seasonal water level is dynamic, the micro-terrain is complex and changeable, has rich natural resources, The study of the wetland ecosystem in the Poyang Lake area has been widely concerned by the scholars in recent years. The study of the wetland soil profile shows that the deep soil may contain a large amount of microbial resources, which has an important effect on the material transformation and energy circulation of the wetland ecosystem. However, for the structure, function and metabolic activity of the microbial community, we know little about the influence of the distribution of the depth profile, and we know little about the system. Therefore, in this study, the soil of Poyang Lake wetland is used as the research object, and three typical areas such as the freshwater mussel (BH), the Changlaway (CHC) and the NJS (NJS) are selected. The depth of the sampling soil is 1 m, which is divided into 5 layers (0-20 cm,20-40 cm,40-60 cm,60-80 cm,80-100 cm). The soil physical and chemical properties, the extracellular enzyme activity, the carbon source metabolic activity, the microbial biomass and the bacterial richness and the community structure were analyzed by a variety of methods such as 16S rRNA high-throughput sequencing, q-PCR, Biolog-ECO and the like. The spatial variation of the structure and the metabolic function of the soil microbial community in the wetland of Poyang Lake, especially the change of the depth of the soil profile, is used to illustrate the soil evolution mechanism for a new route. The results showed that: (1) The main dominant bacteria in the wetland of Poyang Lake were the green lobacter (30.83%), followed by the strain (21.37%) and the acid bacteria (18.85%). The main dominant species were Anabaena (17.49%), followed by Acinetobacter _ (Subgroup _ 1) (9.36%) and HSB _ OF53-F07 (8.00%) at a finer level of classification. At the same time, the bacterial abundance (1.60-108-7.49-1010 copies/ g) and Chao1 index (663-2664) of the wetland in Poyang Lake are also higher than other freshwater wetland ecosystems. The results showed that the metabolic function of the bacterial community in the surface layer (0-20 cm) mainly includes the reduction of sulfate (41.9%), the ammonia oxidation (35.8%), the reduction of nitrite (33.3%) and the dehalogenation (27.7%). The oxidation of the sulfide (25.8%), the degradation of the xylan (20.1%), the fixation of nitrogen (11.0%), etc. The metabolic function of the bacterial community in the deep (20-100 cm) soil mainly includes ammoxidation (37.7%), reduction of sulfate (34.3%), reduction of nitrite (27.8%), Sulphide oxidation (25.7%), dehalogenation (22.9%), methane oxidation (20.0%), and xylan degradation (18.7%). (2) The soil bacterial abundance and community structure of the wetland in Poyang Lake are significantly changed along the soil profile, and the difference is mainly between the soil of the surface layer (0-20cm) and the deep layer (20-100 cm), among which, There was a significant difference in the depth of the soil profile of the wetland. In the aspects of bacterial community metabolism, the function of denitrification (7.0%) and the function of storing polyhydroxybutyrate (0.02%) only existed in the surface soil, while the function of microbial degradation (0.9%) and the function of the selenate reducing agent (0.04%) only existed in the deep soil. It is worth noting that the methane oxidation function (20.00%) in the deep soil is significantly higher than the surface soil (0.08%). Further studies have found that, although there is a significant difference in the structure of the soil bacterial community in different plots, the difference is far from that of the depth. (3) The microbial activity (microbial biomass, extracellular enzyme activity and carbon source metabolism) of the wetland in Poyang Lake was significantly affected by the depth of the soil, and the microbial activity was the highest in the surface layer (0-20 cm) and the soil depth reached 60 cm, and the microbial activity started to be stable. However, the deep soil still carries out complex metabolic activities involving the participation of microorganisms. in addition, only that metabolic activity of the microbial carbon source (AWCD) exist in the sample, the microbial biomass of the soil and the activity of the extracellular enzyme do not change with the sample, indicating that the soil microorganism is a sensitive index of environmental change, and the change of the community structure can take precedence over the change of certain metabolic functions, So as to cope with the change of the external environment. (4) The effect of soil organic matter (AFDM) and nutrient elements (TOC, TN) on the soil microbial function and the structure of the bacterial community was found by the correlation analysis and the redundancy analysis (RDA). In addition, Soil moisture content (SM) is also one of the influencing factors. in which the depth of the AFDM, TOC and TN is significantly reduced along the soil profile, resulting in the functional activity of the soil microorganisms in the Poyang Lake wetland and the main cause of the change of the bacterial community structure with the depth of the soil profile; the difference between the soil SM and the sample ground, The metabolic activity of the soil microbial carbon source and the structure of the bacterial community are changed along with the sample. (5) The activity changes of brayrhizobaiacae,4-29, Xanthomonas campestris (Bxyl), peroxidase (Pero), tomato-grape-glucoamylase (Bglu) and soil phosphatase (Pcos) have a close relationship through the correlation analysis and the redundancy analysis. The activity of Spirochaetacei was related to the activity of glycosaminoglucanase (NAG). Acdothermolaceae,1921-3, HSB _ O53-F07, Cysteobacter, sh765B-TzT-35 are the main species that affect the metabolic activity of the carbon source. In conclusion, the soil properties of the Poyang Lake wetland, the structure of the bacterial community of the soil, the richness of the bacteria and the functional characteristics of the wetland show a significant difference with the depth, and the difference in the horizontal distance is not obvious. Compared with the deep soil (20-100 cm), although the function of the surface soil (0-20cm) is more active and the bacterial abundance is high, the deep soil (even to the depth of 100 cm) still has complex bacterial community composition and higher metabolic function activity, This is not consistent with the vertical distribution of soil microorganisms in other ecosystems. This shows that in the wetland ecosystem, the soil micro-organisms at different depths have different community composition and metabolic functions, and the role of the micro-organisms in the deep soil is of great concern in the soil evolution. The results of this study preliminarily reveal the spatial distribution of soil microorganisms in Poyang Lake wetland and deepen our understanding of the wetland ecosystem. Comprehensive and systematic understanding of wetland ecosystem is an important scientific basis for wetland environmental management, resource protection and sustainable development. This project is characterized by the first use of advanced research means, the spatial distribution of the structure and functional characteristics of the soil microbial community in the wetland of Poyang Lake is systematically studied, and the evolution trend and function activity of the micro-organisms in the wetland ecosystem of Poyang Lake are provided for reference; and the earlier introduction of METAGENE, The high-throughput sequencing results of the 16S rRNA are associated with the community metabolic function to help further understand the functional diversity and importance of the soil microorganisms in the wetland soil along the profile.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：S154.3
本文編號(hào)：2497418
[Abstract]:Poyang Lake wetland is the largest natural freshwater lake wetland in China, the seasonal water level is dynamic, the micro-terrain is complex and changeable, has rich natural resources, The study of the wetland ecosystem in the Poyang Lake area has been widely concerned by the scholars in recent years. The study of the wetland soil profile shows that the deep soil may contain a large amount of microbial resources, which has an important effect on the material transformation and energy circulation of the wetland ecosystem. However, for the structure, function and metabolic activity of the microbial community, we know little about the influence of the distribution of the depth profile, and we know little about the system. Therefore, in this study, the soil of Poyang Lake wetland is used as the research object, and three typical areas such as the freshwater mussel (BH), the Changlaway (CHC) and the NJS (NJS) are selected. The depth of the sampling soil is 1 m, which is divided into 5 layers (0-20 cm,20-40 cm,40-60 cm,60-80 cm,80-100 cm). The soil physical and chemical properties, the extracellular enzyme activity, the carbon source metabolic activity, the microbial biomass and the bacterial richness and the community structure were analyzed by a variety of methods such as 16S rRNA high-throughput sequencing, q-PCR, Biolog-ECO and the like. The spatial variation of the structure and the metabolic function of the soil microbial community in the wetland of Poyang Lake, especially the change of the depth of the soil profile, is used to illustrate the soil evolution mechanism for a new route. The results showed that: (1) The main dominant bacteria in the wetland of Poyang Lake were the green lobacter (30.83%), followed by the strain (21.37%) and the acid bacteria (18.85%). The main dominant species were Anabaena (17.49%), followed by Acinetobacter _ (Subgroup _ 1) (9.36%) and HSB _ OF53-F07 (8.00%) at a finer level of classification. At the same time, the bacterial abundance (1.60-108-7.49-1010 copies/ g) and Chao1 index (663-2664) of the wetland in Poyang Lake are also higher than other freshwater wetland ecosystems. The results showed that the metabolic function of the bacterial community in the surface layer (0-20 cm) mainly includes the reduction of sulfate (41.9%), the ammonia oxidation (35.8%), the reduction of nitrite (33.3%) and the dehalogenation (27.7%). The oxidation of the sulfide (25.8%), the degradation of the xylan (20.1%), the fixation of nitrogen (11.0%), etc. The metabolic function of the bacterial community in the deep (20-100 cm) soil mainly includes ammoxidation (37.7%), reduction of sulfate (34.3%), reduction of nitrite (27.8%), Sulphide oxidation (25.7%), dehalogenation (22.9%), methane oxidation (20.0%), and xylan degradation (18.7%). (2) The soil bacterial abundance and community structure of the wetland in Poyang Lake are significantly changed along the soil profile, and the difference is mainly between the soil of the surface layer (0-20cm) and the deep layer (20-100 cm), among which, There was a significant difference in the depth of the soil profile of the wetland. In the aspects of bacterial community metabolism, the function of denitrification (7.0%) and the function of storing polyhydroxybutyrate (0.02%) only existed in the surface soil, while the function of microbial degradation (0.9%) and the function of the selenate reducing agent (0.04%) only existed in the deep soil. It is worth noting that the methane oxidation function (20.00%) in the deep soil is significantly higher than the surface soil (0.08%). Further studies have found that, although there is a significant difference in the structure of the soil bacterial community in different plots, the difference is far from that of the depth. (3) The microbial activity (microbial biomass, extracellular enzyme activity and carbon source metabolism) of the wetland in Poyang Lake was significantly affected by the depth of the soil, and the microbial activity was the highest in the surface layer (0-20 cm) and the soil depth reached 60 cm, and the microbial activity started to be stable. However, the deep soil still carries out complex metabolic activities involving the participation of microorganisms. in addition, only that metabolic activity of the microbial carbon source (AWCD) exist in the sample, the microbial biomass of the soil and the activity of the extracellular enzyme do not change with the sample, indicating that the soil microorganism is a sensitive index of environmental change, and the change of the community structure can take precedence over the change of certain metabolic functions, So as to cope with the change of the external environment. (4) The effect of soil organic matter (AFDM) and nutrient elements (TOC, TN) on the soil microbial function and the structure of the bacterial community was found by the correlation analysis and the redundancy analysis (RDA). In addition, Soil moisture content (SM) is also one of the influencing factors. in which the depth of the AFDM, TOC and TN is significantly reduced along the soil profile, resulting in the functional activity of the soil microorganisms in the Poyang Lake wetland and the main cause of the change of the bacterial community structure with the depth of the soil profile; the difference between the soil SM and the sample ground, The metabolic activity of the soil microbial carbon source and the structure of the bacterial community are changed along with the sample. (5) The activity changes of brayrhizobaiacae,4-29, Xanthomonas campestris (Bxyl), peroxidase (Pero), tomato-grape-glucoamylase (Bglu) and soil phosphatase (Pcos) have a close relationship through the correlation analysis and the redundancy analysis. The activity of Spirochaetacei was related to the activity of glycosaminoglucanase (NAG). Acdothermolaceae,1921-3, HSB _ O53-F07, Cysteobacter, sh765B-TzT-35 are the main species that affect the metabolic activity of the carbon source. In conclusion, the soil properties of the Poyang Lake wetland, the structure of the bacterial community of the soil, the richness of the bacteria and the functional characteristics of the wetland show a significant difference with the depth, and the difference in the horizontal distance is not obvious. Compared with the deep soil (20-100 cm), although the function of the surface soil (0-20cm) is more active and the bacterial abundance is high, the deep soil (even to the depth of 100 cm) still has complex bacterial community composition and higher metabolic function activity, This is not consistent with the vertical distribution of soil microorganisms in other ecosystems. This shows that in the wetland ecosystem, the soil micro-organisms at different depths have different community composition and metabolic functions, and the role of the micro-organisms in the deep soil is of great concern in the soil evolution. The results of this study preliminarily reveal the spatial distribution of soil microorganisms in Poyang Lake wetland and deepen our understanding of the wetland ecosystem. Comprehensive and systematic understanding of wetland ecosystem is an important scientific basis for wetland environmental management, resource protection and sustainable development. This project is characterized by the first use of advanced research means, the spatial distribution of the structure and functional characteristics of the soil microbial community in the wetland of Poyang Lake is systematically studied, and the evolution trend and function activity of the micro-organisms in the wetland ecosystem of Poyang Lake are provided for reference; and the earlier introduction of METAGENE, The high-throughput sequencing results of the 16S rRNA are associated with the community metabolic function to help further understand the functional diversity and importance of the soil microorganisms in the wetland soil along the profile.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：S154.3
【參考文獻(xiàn)】
相關(guān)期刊論文 前10條
1 隋心;張榮濤;楊立賓;許楠;鐘海秀;王繼豐;倪紅偉;;三江平原不同類(lèi)型小葉章濕地土壤細(xì)菌群落功能多樣性[J];環(huán)境科學(xué)研究;2016年10期
2 王鵬;陳波;張華;;基于高通量測(cè)序的鄱陽(yáng)湖典型濕地土壤細(xì)菌群落特征分析[J];生態(tài)學(xué)報(bào);2017年05期
3 陳熙;劉以珍;李金前;葛剛;吳蘭;李永繡;;稀土尾礦土壤細(xì)菌群落結(jié)構(gòu)對(duì)植被修復(fù)的響應(yīng)[J];生態(tài)學(xué)報(bào);2016年13期
4 馬曉飛;楚新正;馬倩;;艾比湖地區(qū)融雪期梭梭和檉柳林地土壤酶活性研究[J];中國(guó)農(nóng)學(xué)通報(bào);2016年14期
5 李艷紅;葛剛;王茂林;周曉嵐;胡春華;;垂向歸納模型下鄱陽(yáng)湖豐、枯水期初級(jí)生產(chǎn)力特征及與環(huán)境因子相關(guān)性分析[J];湖泊科學(xué);2016年03期
6 韓曉君;;三江平原濕地生態(tài)系統(tǒng)保護(hù)研究進(jìn)展[J];黑龍江水利科技;2016年04期
7 楊英;耿玉清;黃桂林;趙恒毅;周紅娟;;青海小泊湖區(qū)沼澤化草甸、草甸和沙地的土壤酶活性[J];濕地科學(xué);2016年01期
8 王金爽;胡泓;李甜甜;李正炎;呂向梨;;環(huán)境因素對(duì)濕地土壤微生物群落影響研究進(jìn)展[J];濕地科學(xué)與管理;2015年04期
9 蔣紹妍;王文星;薛向欣;徐坤;;利用PCR-DGGE分析茂名油頁(yè)巖礦區(qū)土壤細(xì)菌群落組成[J];中南大學(xué)學(xué)報(bào)(自然科學(xué)版);2015年12期
10 隋心;張榮濤;鐘海秀;許楠;王繼豐;劉應(yīng)竹;袁海峰;倪紅偉;;利用高通量測(cè)序?qū)θ皆∪~章濕地土壤細(xì)菌多樣性的研究[J];土壤;2015年05期
相關(guān)博士學(xué)位論文 前2條
1 陳偉;納帕海高原濕地微生物多樣性研究[D];昆明理工大學(xué);2016年
2 賈玉華;坡面土壤水分時(shí)空變異的試驗(yàn)研究[D];中國(guó)科學(xué)院研究生院（教育部水土保持與生態(tài)環(huán)境研究中心）;2013年
相關(guān)碩士學(xué)位論文 前5條
1 張祖芳;鄱陽(yáng)湖濕地生態(tài)功能重要性分區(qū)研究[D];南昌大學(xué);2014年
2 國(guó)春菲;土壤鹽分和pH對(duì)濱海鹽土土壤微生物多樣性的影響[D];浙江農(nóng)林大學(xué);2013年
3 陳會(huì)海;三江濕地土壤微生物群落結(jié)構(gòu)的多樣性分析[D];東北農(nóng)業(yè)大學(xué);2013年
4 胡維;鄱陽(yáng)湖南磯山濕地土壤微生物群落結(jié)構(gòu)的時(shí)空變化特點(diǎn)研究[D];南昌大學(xué);2012年
5 孟晗;長(zhǎng)三角地區(qū)土壤不同發(fā)育階段微生物群落結(jié)構(gòu)的變化[D];復(fù)旦大學(xué);2011年
，本文編號(hào)：2497418
本文鏈接：http://www.sikaile.net/kejilunwen/nykj/2497418.html
最近更新
教材專(zhuān)著
