【摘要】：研究了反相高效液相色譜法同時(shí)測(cè)定濕地植物根際土壤溶液中11種低分子有機(jī)酸的優(yōu)化方法。探索了柱溫、波長(zhǎng)、流速、流動(dòng)相中的pH值以及有機(jī)改良劑對(duì)檢測(cè)效果的影響,并進(jìn)一步驗(yàn)證方法的精密度、加標(biāo)回收率、檢測(cè)限等指標(biāo)。結(jié)果表明,采用Waters Atlantis T3 C18(4.6×250 mm,5μm)色譜柱,在柱溫為35℃,紫外檢測(cè)波長(zhǎng)210 nm,流動(dòng)相為磷酸鹽緩沖液(0.01 mol/L,pH 2.5)與乙腈(ACN)混合溶液,在流速0.7 m L/min時(shí),進(jìn)行梯度洗脫分離。該方法加標(biāo)回收率在95.6%~103.9%,相對(duì)標(biāo)準(zhǔn)偏差在0.3%~5.2%,各酸峰面積與質(zhì)量濃度線性關(guān)系良好(R2≥0.999)。該方法成功用于介質(zhì)復(fù)雜的環(huán)境樣品中低分子有機(jī)酸的檢測(cè),2014年4月采集的2種濕地挺水植物根際與非根際土壤溶液檢測(cè)主要存在草酸與丙二酸。蘆葦土壤溶液中草酸總計(jì)15.83 mg/L,丙二酸總計(jì)18.10 mg/L;扁稈呰草土壤溶液中草酸17.90 mg/L,丙二酸總計(jì)17.05 mg/L。2種植物中,根際土壤溶液中丙二酸含量均高于非根際土壤溶液。
[Abstract]:An optimized method for simultaneous determination of 11 low molecular weight organic acids in rhizosphere soil solutions of wetland plants by reversed phase high performance liquid chromatography (RP-HPLC) was studied. The effects of column temperature, wavelength, flow rate, pH value in mobile phase and organic improver on the detection effect were explored. The precision, recovery rate and detection limit of the method were further verified. The results showed that a Waters Atlantis T3 C18 (4.6 脳 250 mm, 5 渭 m) column was used, the column temperature was 35 鈩，

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