微生物源揮發(fā)性化合物苯并噻唑?qū)颐共【囊种菩?/H1>
發(fā)布時間：2018-11-01 18:13
【摘要】：【目的】評價揮發(fā)性化合物苯并噻唑防治灰霉病的應用潛力,為進一步開發(fā)利用提供依據(jù)�！痉椒ā坎捎妹芊獗P菌絲生長速率法測定山東省73株灰霉病菌(Botrytis cinerea)對苯并噻唑的敏感性,并比較不同地區(qū)和不同表現(xiàn)型菌株(多菌靈抗性和敏感菌株:Car~R,Car~S;腐霉利抗性和敏感菌株:Prc~R,Prc~S;嘧霉胺抗性和敏感菌株:Pyr~R,Pyr~S;啶酰菌胺抗性和敏感菌株:Bos~R,Bos~S)間的敏感性差異;測定苯并噻唑與多菌靈、啶酰菌胺室內(nèi)混用對灰霉病菌的抑制作用。通過離體黃瓜葉片接種試驗驗證苯并噻唑?qū)S瓜灰霉病的保護、治療效果以及不同熏蒸劑量和時間對灰霉病菌致病力的影響,并用掃描電子顯微鏡觀察苯并噻唑?qū)颐咕z形態(tài)的影響�！窘Y果】苯并噻唑?qū)颐共【z的EC50范圍為0.38—1.08μL·L~(-1),均值為0.62μL·L~(-1),不同地區(qū)之間無敏感性差異;山東地區(qū)多菌靈、腐霉利、嘧霉胺、啶酰菌胺抗性和敏感灰霉病菌對苯并噻唑均比較敏感,說明這4種殺菌劑與苯并噻唑無交互抗性;在苯并噻唑0.1—3μL·L~(-1)范圍內(nèi),菌絲比孢子對其更敏感;2μL·L~(-1)苯并噻唑?qū)z的抑制率高達86.12%,對芽管伸長的抑制率為55.15%,而對孢子萌發(fā)的抑制率僅為19.30%�？梢�,病菌不同生長階段對此化合物的敏感性不同。苯并噻唑與多菌靈混用防治黃瓜灰霉病無增效或相加作用,兩者混用時哪種藥劑發(fā)揮主要抑菌作用與各組分在混用中使用的濃度和比例有關;苯并噻唑與啶酰菌胺混用有顯著增效作用,其田間實際應用方式、效果以及增效機制還有待進一步明確。離體葉片試驗表明,苯并噻唑?qū)S瓜灰霉病兼具保護和治療防效,2μL·L~(-1)的治療防效為91.01%,高于對照藥劑啶酰菌胺300 mg·L~(-1)(78.90%),并顯著高于48.25%的保護防效。苯并噻唑能夠顯著降低灰霉病菌菌絲的致病力,受抑制程度與苯并噻唑濃度呈正相關。掃描電鏡觀察發(fā)現(xiàn),苯并噻唑能夠使菌絲形態(tài)異常,菌絲干癟,分枝增多,表面凹陷�！窘Y論】苯并噻唑?qū)ι綎|省不同地區(qū)的灰霉病菌均有較高的毒力,適合作為防治灰霉病的熏蒸劑使用。
[Abstract]:[objective] to evaluate the application potential of benzothiazole, a volatile compound, in the control of cinerea. [methods] the sensitivity of 73 strains of Botrytis cinerea (Botrytis cinerea) to benzothiazole in Shandong Province was determined by the method of the growth rate of hermetically sealed hyphae. And compare different regions and different phenotypic strains (carbendazim resistance and sensitive strains: Car~R,Car~S; The difference of sensitivity between the resistant and sensitive strains of Pythium sp. Prc~R,Prc~S; and that of Pyr~R,Pyr~S; and Bos~R,Bos~S; The inhibitory effects of benzothiazole, carbendazim and acyl chloride on Botrytis cinerea were determined. The effects of benzothiazole on the protection of cinerea and the effects of different fumigation doses and time on the pathogenicity of cinerea were tested by inoculation of cucumber leaves in vitro. The effects of benzothiazole on the morphology of Aspergillus cinerea were observed by scanning electron microscope. [results] the EC50 range of benzothiazole on the hyphae of the fungus was 0.38-1.08 渭 L L ~ (-1), and the mean value was 0.62 渭 L L ~ (-1). There was no difference in sensitivity between different regions. Carbendazim, Pythium, pyridamine, pyridamine and susceptible cinerea were all sensitive to benzothiazole, indicating that these four fungicides had no cross resistance to benzothiazole. In the range of 0.1-3 渭 L L ~ (-1) of benzothiazole, mycelium is more sensitive than spores. The inhibition rate of 2 渭 L L ~ (-1) benzothiazole on hyphae was 86.12, the inhibition rate on tube elongation was 55.15, and the inhibition rate on spore germination was 19.30%. It can be seen that the susceptibility of bacteria to this compound is different at different growth stages. The mixture of benzothiazole and carbendazim had no synergistic or additive effect on the control of cinerea in cucumber. The main bacteriostatic effect of benzothiazole and carbendazim was related to the concentration and proportion of each component used in the mixture. The combination of benzothiazole and acylamine has a significant synergistic effect, and its practical application mode, effect and synergistic mechanism need to be further clarified. In vitro leaf tests showed that benzothiazole had both protective and therapeutic effects on cucumber grey mold. The control effect of 2 渭 L L ~ (-1) was 91.01%, which was higher than that of the control drug of 300 mg L ~ (-1) (78.90%). The protective effect was significantly higher than that of 48.25%. Benzothiazole could significantly reduce the pathogenicity of the mycelium of Botrytis cinerea, and the degree of inhibition was positively correlated with the concentration of benzothiazole. It was found by SEM that benzothiazole could make hyphal morphology abnormal, mycelium shriveled, branching increased and surface hollow. [conclusion] benzothiazole has high virulence to different areas of grey mold bacteria in Shandong Province. It is suitable for use as fumigation agent to control cinerea.
【作者單位】： 山東農(nóng)業(yè)大學植物保護學院;山東農(nóng)業(yè)大學山東省蔬菜病蟲生物學重點實驗室;山東省農(nóng)藥毒理與應用技術省級重點實驗室;
【基金】：國家重點研發(fā)計劃(2016YFD0200500)
【分類號】：S432.4
【相似文獻】
相關期刊論文 前1條
1 劉潔;宋功武;;3-乙基苯并噻唑螺萘并VA嗪與牛血清白蛋白的相互作用[J];化學研究;2013年06期
，
本文編號：2304699
本文鏈接：http://www.sikaile.net/kejilunwen/nykj/2304699.html
[Abstract]:[objective] to evaluate the application potential of benzothiazole, a volatile compound, in the control of cinerea. [methods] the sensitivity of 73 strains of Botrytis cinerea (Botrytis cinerea) to benzothiazole in Shandong Province was determined by the method of the growth rate of hermetically sealed hyphae. And compare different regions and different phenotypic strains (carbendazim resistance and sensitive strains: Car~R,Car~S; The difference of sensitivity between the resistant and sensitive strains of Pythium sp. Prc~R,Prc~S; and that of Pyr~R,Pyr~S; and Bos~R,Bos~S; The inhibitory effects of benzothiazole, carbendazim and acyl chloride on Botrytis cinerea were determined. The effects of benzothiazole on the protection of cinerea and the effects of different fumigation doses and time on the pathogenicity of cinerea were tested by inoculation of cucumber leaves in vitro. The effects of benzothiazole on the morphology of Aspergillus cinerea were observed by scanning electron microscope. [results] the EC50 range of benzothiazole on the hyphae of the fungus was 0.38-1.08 渭 L L ~ (-1), and the mean value was 0.62 渭 L L ~ (-1). There was no difference in sensitivity between different regions. Carbendazim, Pythium, pyridamine, pyridamine and susceptible cinerea were all sensitive to benzothiazole, indicating that these four fungicides had no cross resistance to benzothiazole. In the range of 0.1-3 渭 L L ~ (-1) of benzothiazole, mycelium is more sensitive than spores. The inhibition rate of 2 渭 L L ~ (-1) benzothiazole on hyphae was 86.12, the inhibition rate on tube elongation was 55.15, and the inhibition rate on spore germination was 19.30%. It can be seen that the susceptibility of bacteria to this compound is different at different growth stages. The mixture of benzothiazole and carbendazim had no synergistic or additive effect on the control of cinerea in cucumber. The main bacteriostatic effect of benzothiazole and carbendazim was related to the concentration and proportion of each component used in the mixture. The combination of benzothiazole and acylamine has a significant synergistic effect, and its practical application mode, effect and synergistic mechanism need to be further clarified. In vitro leaf tests showed that benzothiazole had both protective and therapeutic effects on cucumber grey mold. The control effect of 2 渭 L L ~ (-1) was 91.01%, which was higher than that of the control drug of 300 mg L ~ (-1) (78.90%). The protective effect was significantly higher than that of 48.25%. Benzothiazole could significantly reduce the pathogenicity of the mycelium of Botrytis cinerea, and the degree of inhibition was positively correlated with the concentration of benzothiazole. It was found by SEM that benzothiazole could make hyphal morphology abnormal, mycelium shriveled, branching increased and surface hollow. [conclusion] benzothiazole has high virulence to different areas of grey mold bacteria in Shandong Province. It is suitable for use as fumigation agent to control cinerea.
【作者單位】： 山東農(nóng)業(yè)大學植物保護學院;山東農(nóng)業(yè)大學山東省蔬菜病蟲生物學重點實驗室;山東省農(nóng)藥毒理與應用技術省級重點實驗室;
【基金】：國家重點研發(fā)計劃(2016YFD0200500)
【分類號】：S432.4
【相似文獻】
相關期刊論文 前1條
1 劉潔;宋功武;;3-乙基苯并噻唑螺萘并VA嗪與牛血清白蛋白的相互作用[J];化學研究;2013年06期
，本文編號：2304699
本文鏈接：http://www.sikaile.net/kejilunwen/nykj/2304699.html
最近更新
教材專著
