【摘要】：為探討斑馬魚胚胎暴露于丙草胺后對斑馬魚仔魚的致畸效應和致畸機理,研究了斑馬魚胚胎暴露于0.50、1.00、1.50 mg·L~(-1)丙草胺染毒液120 h后仔魚的畸形表型及比率,并通過檢測與畸形器官發(fā)育相關(guān)的基因表達探討致畸機理。結(jié)果發(fā)現(xiàn),丙草胺暴露后誘導斑馬魚仔魚出現(xiàn)心包囊腫、軀干彎曲、游囊關(guān)閉等畸形表型,隨著暴露濃度的增加,各畸形癥狀比例增大。1.00、1.50 mg·L~(-1)暴露組仔魚心包囊腫率分別為15.56%(P0.05)、25.56%(P0.01),脊柱彎曲率分別為27.78%(P0.01)、35.56%(P0.01);0.50、1.00、1.50 mg·L~(-1)暴露組仔魚游囊關(guān)閉率分別為20%(P0.05)、37.78%(P0.01)和60%(P0.01)。丙草胺顯著降低了心臟發(fā)育相關(guān)基因Tbx2[1.00 mg·L~(-1)(P0.01)、1.50 mg·L~(-1)(P0.05)],骨骼發(fā)育相關(guān)基因BMP-2[1.50 mg·L~(-1)(P0.05)]、BMP-4[1.00 mg·L~(-1)(P0.01)、1.50 mg·L~(-1)(P0.01)],游囊發(fā)育相關(guān)基因shha[1.50 mg·L~(-1)(P0.05)]、ihha[1.00 mg·L~(-1)(P0.05)、1.50 mg·L~(-1)(P0.05)]的表達水平。研究表明,丙草胺通過影響心臟、骨骼和游囊相關(guān)基因的表達引起斑馬魚仔魚的以上器官發(fā)育異常。
[Abstract]:In order to study the teratogenicity and teratogenic mechanism of zebrafish embryos exposed to propyloxifen, the abnormal phenotype and rate of zebrafish embryos exposed to 1.50 mg L ~ (-1) propanolamine for 120h were studied. The mechanism of teratogenicity was discussed by detecting the gene expression related to the development of malformed organs. The results showed that malformed phenotypes such as pericardial cysts, torso curvature and cysts closed were induced by propanoxifen in zebrafish larvae after exposure, and with the increase of exposure concentration, The rate of pericardial cyst was 15.56% (P0.05), 25.56% (P0.01), 27.78% (P0.01), 35.56% (P0.01) in 1.50 mg L-1 exposure group, and 20% (P0.05), 37.78% (P0.01) and 60% (P0.01) in 0.500.001.50 mg L-1 exposure group, respectively. 涓欒崏鑳烘樉钁楅檷浣庝簡蹇冭剰鍙戣偛鐩稿叧鍩哄洜Tbx2[1.00 mg路L~(-1)(P0.01),1.50 mg路L~(-1)(P0.05)],楠ㄩ鍙戣偛鐩稿叧鍩哄洜BMP-2[1.50 mg路L~(-1)(P0.05)],BMP-4[1.00 mg路L~(-1)(P0.01),1.50 mg路L~(-1)(P0.01)],娓稿泭鍙戣偛鐩稿叧鍩哄洜shha[1.50 mg路L~(-1)(P0.05)],ihha[1.00 mg路L~(-1)(P0.05),1.50 mg路L~(-1)(P0.05)]鐨勮〃杈炬按騫，

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