4種蟲生真菌對儲糧害蟲黃粉蟲不同蟲態(tài)的生防研究
本文關(guān)鍵詞: 蟲生真菌 儲糧害蟲 黃粉蟲 毒力 生物防治 出處:《西南農(nóng)業(yè)學(xué)報》2017年08期 論文類型:期刊論文
【摘要】:【目的】通過研究蟲生真菌對黃粉蟲不同蟲態(tài)的生防效果,以期為儲糧害蟲的生物防治提供參考!痉椒ā吭趯嶒炇夷M干燥的儲糧環(huán)境,測定了4種蟲生真菌菌株(金龜子綠僵菌MAX-2、玫煙色棒束孢IFK-20、球孢白僵菌BBL-3、蟬棒束孢ICG-1)對黃粉蟲3種不同蟲態(tài)(8齡幼蟲、蛹、剛羽化的成蟲)的生防效果。【結(jié)果】在模擬儲糧環(huán)境中,BBL-3對3種蟲態(tài)的毒力都較低,說明該菌株不適宜在干燥條件下發(fā)揮作用;MAX-2對幼蟲和成蟲的毒力都最高,IFK-20其次;孢子懸液處理蛹,ICG-1致死速度最快,其次為MAX-2與IFK-20;ICG-1在模擬儲糧環(huán)境中對幼蟲和成蟲無毒力,但用其孢子懸液處理蛹,毒力卻達到最高,說明蟲生真菌有的對不同蟲態(tài)的毒力較穩(wěn)定,而有的可能只在昆蟲某些發(fā)育階段或在特定環(huán)境中發(fā)揮有效作用。對MAX-2侵染黃粉蟲不同蟲態(tài)的特征進行了描述,發(fā)現(xiàn)其防治害蟲的效果具有持續(xù)性,部分被感染的幼蟲和蛹可以帶菌生存,但生活力下降,有的發(fā)育畸形,最終都會死亡!窘Y(jié)論】不同蟲生真菌菌株對不同蟲態(tài)的毒力差異顯著,同一菌株對不同蟲態(tài)的毒力也不同。除ICG-1外其它3株菌在黃粉蟲不同發(fā)育階段都具有較好防效,MAX-2對3種蟲態(tài)的持續(xù)防效最好,有望應(yīng)用于儲糧害蟲的生物防治。
[Abstract]:[objective] to study the biocontrol effects of insecticidal fungi on different species of powdery mildew in order to provide a reference for biological control of stored grain pests. [methods] the dry storage environment was simulated in laboratory. The effects of four insecticidal fungi strains (Metarhizium anisopliae MAX-2, Rosa fumigatus IFK-20, Beauveria bassiana BBL-3, Coryosporium cicadae ICG-1) on the eighth instar larvae and pupae of three different species of Molitor's larvae were determined. [results] the virulence of BBL-3 to three species of insects was lower in simulated grain storage environment, which indicated that the strain was not suitable to act under dry conditions. The virulence of MAX-2 to larvae and adults was the highest, followed by IFK-20. The lethal rate of ICG-1 was the fastest when treated with spore suspension, followed by MAX-2 and IFK-20 ICG-1, which had no toxicity to larvae and adults in simulated grain storage environment, but the virulence reached the highest when the spores suspension was used to treat pupae. It is suggested that some insecticidal fungi have stable virulence to different insect forms, while others may only play an effective role in certain stages of insect development or in specific environment. The characteristics of MAX-2 infecting different species of powdery mildew are described in this paper. It was found that the control effect of insect pests was sustainable, and some infected larvae and pupae could survive with bacteria, but their viability decreased and some of them developed malformation. [conclusion] the virulence of different insecticidal fungi to different insect forms is significant. The virulence of the same strain to different insect forms was also different. Except ICG-1, the other three strains had better control effect at different stages of development of powdery mildew. MAX-2 had the best sustained control effect on the three species of insects, which was expected to be applied to biological control of stored grain pests.
【作者單位】: 保山學(xué)院高黎貢山生物資源研究所;曲靖師范學(xué)院化學(xué)化工學(xué)院;
【基金】:國家自然科學(xué)地區(qū)科學(xué)基金項目(31460153) 保山市科技計劃項目(2014KJ033) 保山市第六批中青年學(xué)術(shù)和技術(shù)帶頭人(bszqnxshjsdtr2017) 云南省科技廳青年項目(2014FD057) 保山學(xué)院校級項目(BZ15006)
【分類號】:S379.5;S476.12
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