本文關(guān)鍵詞： 菹草 內(nèi)生菌 異養(yǎng)硫氧化 硫化氫 殺鮭氣單胞菌 雞糞　出處：《吉林農(nóng)業(yè)大學(xué)學(xué)報(bào)》2017年02期 　論文類型：期刊論文

【摘要】：雞糞中的含硫有機(jī)物在微生物作用下會(huì)釋放出大量的硫化氫氣體,危害畜禽的健康及污染環(huán)境。試驗(yàn)以減少雞糞中硫化氫釋放量為目的,從菹草內(nèi)分離得到1株具有異養(yǎng)硫氧化作用的細(xì)菌,命名為JS3。16S rRNA序列分析表明菌株JS3與殺鮭氣單胞菌的親源關(guān)系最近。以硫代硫酸鈉為唯一硫源時(shí)菌株JS3的硫氧化特征:碳源優(yōu)先利用順序?yàn)槠咸烟菣幟仕徕c乙酸鈉;氮源優(yōu)先利用順序?yàn)橄跛徕c氯化銨;菌株JS3氧化硫的最佳碳氮比為15∶1,此時(shí)77.82%的硫代硫酸根被轉(zhuǎn)化為硫酸根;pH從4.05增加到10.05,菌株的氧化硫能力先增強(qiáng)后下降,當(dāng)pH為9.05時(shí),硫酸根的積累量最高,可達(dá)到4.33 g/L;當(dāng)氯化鈉添加量達(dá)到4.0%時(shí)對(duì)菌株JS3才產(chǎn)生明顯影響,說明株菌具有一定的耐鹽性;最佳硫代硫酸根初始濃度為2 810 mg/L,此時(shí)83.22%的硫代硫酸根被轉(zhuǎn)化為硫酸根;250 mL三角瓶中裝液量為75 mL時(shí),菌株JS3是硫氧化能力最強(qiáng)。在硫化氫減釋模擬試驗(yàn)中,與對(duì)照相比,菌株JS3可降低84.53%的硫化氫釋放量。
[Abstract]:The sulfur organic matter in chicken manure can release a large amount of hydrogen sulfide gas under the action of microorganism, which will harm the health of livestock and poultry and pollute the environment. The purpose of the experiment is to reduce the release of hydrogen sulfide from chicken manure. A strain of bacteria with heterotrophic sulfur oxidation was isolated from Potamogpus crispus. The sequence analysis of JS3.16S rRNA showed that the relationship between strain JS3 and Aeromonas salmon was close. When sodium thiosulfate was used as the sole sulfur source, the characteristics of sulfur oxidation of strain JS3 were observed. The priority of carbon source was glucose, sodium citrate, sodium acetate; The priority order of nitrogen use was sodium nitrate and ammonium chloride; The optimum ratio of carbon to nitrogen for sulfur oxidation of strain JS3 was 15: 1, and 77.82% thiosulfate was converted to sulfate. When pH increased from 4.05 to 10.05, the sulfur oxidation ability of the strain increased first and then decreased. When pH was 9.05, the accumulation of sulfate was the highest, reaching 4.33 g 路L ~ (-1); When the amount of sodium chloride reached 4.0, the strain JS3 had obvious influence, which indicated that the strain had certain salt tolerance. The optimum initial concentration of thiosulfate was 2 810 mg / L, and 83.22% thiosulfate was converted to sulfate. When the volume of liquid in 250ml triangle bottle was 75 mL, the strain JS3 was the most capable of sulfur oxidation. Strain JS3 can reduce hydrogen sulfide release of 84.53%.
【作者單位】： 吉林農(nóng)業(yè)大學(xué)資源與環(huán)境學(xué)院;長(zhǎng)春市農(nóng)業(yè)科學(xué)院;吉林省商品糧基地土壤資源可持續(xù)利用重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(51109089) 吉林省科技發(fā)展計(jì)劃項(xiàng)目(20130206031NY,20160204025SF) 長(zhǎng)春市科技計(jì)劃項(xiàng)目(13NK29)
【分類號(hào)】：X172;X713
【正文快照】： 商品糧基地土壤資源可持續(xù)利用重點(diǎn)實(shí)驗(yàn)室,長(zhǎng)春130118引文格式:王金英,秦玉瑩,陳華晶,等.菹草內(nèi)生硫氧化細(xì)菌的分離鑒定及對(duì)雞糞中硫化氫的減釋作用[J].吉林農(nóng)業(yè)大學(xué)學(xué)報(bào),2017,39(2):176-182.***通信作者priority utilization order of strain JS3 to oxidize sulfur was ammo

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