本文關鍵詞：耕作方式和秸桿還田對纖維素降解細菌多樣性的影響　出處：《土壤學報》2016年04期 　論文類型：期刊論文

  更多相關文章： 免耕 秸稈還田 纖維素降解細菌 多樣性 PCR-RFLP

【摘要】：免耕和秸稈還田是培肥地力的重要措施,明確其對土壤纖維素降解細菌群落的影響對于土壤質量提升具有重要意義。選擇中國科學院封丘農業(yè)生態(tài)國家試驗站耕作秸稈還田(WtS)、免耕秸稈還田(WntS)、耕作秸稈不還田(Wt)和免耕秸稈不還田(Wnt)4種處理小區(qū)并采集土壤樣品,用CMC-Na剛果紅培養(yǎng)基對纖維素降解細菌計數,采用PCR-RFLP技術研究保護性耕作和秸稈還田對纖維素降解細菌群落的影響。結果表明,秸稈還田與免耕處理均不同程度提高了纖維素降解細菌數量。WtS、WntS、Wt和Wnt4個纖維素降解細菌基因文庫中,OTUs數量分別為23、26、20和19個,秸稈還田加免耕處理多樣性指數最高。本文獲得的纖維素降解細菌共屬于11個屬,秸稈還田土壤中Streptomycetaceae、Flavobacterium、Sphingobium相對豐度明顯多于秸稈不還田處理土壤;免耕處理土壤中Pseudomonas、Phyllobacterium、Paenibacillus、Promicromonosporaceae、Sphingobacterium相對豐度明顯高于耕作處理土壤。典范對應分析顯示四種處理纖維素降解細菌群落結構發(fā)生了較大變化,p H、全磷、有機碳和全鉀與免耕處理呈顯著正相關性,全氮、堿解氮、速效鉀、速效磷和有機碳與秸稈還田處理呈顯著正相關性。免耕和秸稈還田能提高土壤中纖維素降解細菌數量及多樣性,改變纖維素降解細菌群落結構。
[Abstract]:No-tillage and straw returning to the field are important measures to increase fertility. It is of great significance to improve soil quality by determining its effect on soil cellulose degradation bacteria community. Select the National Experimental Station of Fengqiu Agroecology of Chinese Academy of Sciences to return straw to field WtSs). No tillage straw returned to field WNT Sn, tillage straw did not return to field Wt) and no tillage straw did not return to field WntN 4 kinds of treatment plots and collected soil samples. The cellulose-degrading bacteria were counted in CMC-Na Congo red medium. The effects of conservation tillage and straw returning on the cellulose-degrading bacteria community were studied by PCR-RFLP technique. Straw returning and no-tillage treatments increased the number of cellulose-degrading bacteria in different degree. The number of OTUs was 23 in the gene library of cellulose degrading bacteria (WtSX WntSN Wt and Wnt4), respectively. The diversity index of 20 and 19 straw returning treatments with no-tillage treatment was the highest. The cellulose degrading bacteria obtained in this paper belong to 11 genera. The relative abundance of Streptomycetaceaeus Flavobacteriumum Sphingobium in straw returning soil was significantly higher than that in soil without straw treatment. Pseudomonas spp. Phyllobacterium Paenibacillus. Promicromonosporaceae. The relative abundance of Sphingobacterium was significantly higher than that of tillage treatment soil. The canonical correspondence analysis showed that the community structure of cellulose-degrading bacteria in the four treatments had changed greatly. Organic carbon and total potassium were positively correlated with no-tillage treatment, including total nitrogen, alkali-hydrolyzed nitrogen and available potassium. There was a significant positive correlation between available phosphorus and organic carbon and straw returning treatment, which could increase the number and diversity of cellulose degrading bacteria in soil and change the community structure of cellulose degrading bacteria.
【作者單位】： 南京農業(yè)大學生命科學學院/農業(yè)部農業(yè)環(huán)境微生物工程重點實驗室;封丘農業(yè)生態(tài)國家試驗站土壤與農業(yè)可持續(xù)發(fā)展國家重點實驗室(中國科學院南京土壤研究所);
【基金】：國家重點基礎研究發(fā)展計劃項目(2011CB100504) 國家自然科學基金項目(41371262,41471239)資助~~
【分類號】：S345;S141.4
【正文快照】： 纖維素是一類重要的生物高聚物,占植物干重的30%~50%[1],它們隨植物殘體進入土壤后,為土壤微生物生長和繁殖提供豐富的碳源。不同種屬的微生物產生不同的酶系[2],因而不同微生物來源的酶所催化的有機底物特異性有很大差別[3];此外,由于土壤中存在的纖維素種類繁多、結構復雜和

【相似文獻】

相關期刊論文 前10條

1 宋惠月;高建梅;盧月霞;;纖維素降解放線菌的篩選及其降解效果研究[J];江蘇農業(yè)科學;2013年04期

2 徐有權;顧文杰;張發(fā)寶;徐培智;解開治;王立群;;酸性半纖維素降解細菌的篩選與鑒定[J];微生物學雜志;2012年02期

3 趙菁;黃奮飛;林生;林文雄;;纖維素降解細菌的分離、鑒定及其系統(tǒng)發(fā)育分析[J];安徽農學通報(上半月刊);2011年17期

4 孫浩;繆�？�;李彪;;兩株芭蕉花內生菌纖維素降解能力的測定[J];吉林農業(yè);2011年05期

5 范黎;;纖維素高效降解菌的篩選[J];微生物學通報;2012年05期

6 劉發(fā)來;范建平;蘇士;張子義;;纖維素生物轉化研究進展[J];安徽農業(yè)科學;2008年17期

7 馬寧;王彥杰;王文旭;曹雪梅;王心;胡小平;;纖維素降解真菌的篩選與鑒定[J];西北農業(yè)學報;2012年08期

8 韓如e，

本文編號：1392599