【摘要】：【目的】以鹽生植物鹽穗木為材料,研究不同引物對和不同退火溫度對鹽穗木內(nèi)參基因β-actin擴增效率的影響。【方法】設(shè)計擴增β-actin基因的兩對引物,標記為β-actin1和β-actin2,分別建立這兩對引物擴增鹽穗木β-actin基因和特異性引物擴增該物種的過氧化物酶基因POD(鹽響應(yīng)的代表性基因),在不同退火溫度下的標準曲線和擴增曲線�！窘Y(jié)果】不論55還是58℃退火溫度,引物對β-actin1比引物對β-actin2有更好的擴增效率;在55℃退火溫度下,引物對β-actin1有更高的擴增效率。在這兩個退火溫度下,分別以β-actin1和β-actin2引物對擴增β-actin基因作為內(nèi)參,鹽穗木POD基因的相對表達水平具有一定的差異性。【結(jié)論】引物和退火溫度會影響熒光定量PCR的擴增效率,進而會影響靶基因的相對表達水平。
[Abstract]:[objective] to study the effects of different primer pairs and annealing temperature on the amplification efficiency of 尾-actin gene in halophyte salted wood. [methods] two pairs of primers for amplification of 尾-actin gene were designed. 尾-actin1 and 尾-actin2, were used to amplify 尾-actin gene and specific primer POD (representative gene of salt response), respectively. The standard curve and amplification curve at different annealing temperatures. [results] the amplification efficiency of primer pair 尾-actin1 was better than that of primer pair 尾-actin2 regardless of 55 鈩，

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