【摘要】：目的探討肺腺癌程序性死亡分子-1(programmed death 1,PD-1)、程序性死亡分子配體-1(programmed death ligand 1,PD-L1)蛋白的表達與K-RAS基因突變的相關(guān)性。方法采用免疫組化EnVision兩步法檢測PD-1、PD-L1蛋白的表達,應用實時熒光定量PCR技術(shù)檢測K-RAS基因的突變類型。結(jié)果肺腺癌組織中PD-1、PD-L1的陽性率均高于良性病變肺組織(P0.01),PD-1、PD-L1蛋白表達與患者性別、年齡、吸煙史、分化程度、淋巴結(jié)轉(zhuǎn)移及TNM分期均無相關(guān)性(P0.05)。36例肺腺癌樣本中發(fā)生K-RAS基因突變者8例(22.2%),K-RAS基因突變與患者性別、年齡、吸煙史、分化程度、淋巴結(jié)轉(zhuǎn)移及TNM分期均無關(guān)(P0.05)。相關(guān)分析顯示PD-1、PD-L1蛋白表達與K-RAS突變無關(guān)(P0.05)。結(jié)論 PD-1、PD-L1蛋白在肺腺癌中的表達明顯高于良性病變肺組織,但兩者表達程度與肺腺癌的臨床病理特征及K-RAS基因突變無關(guān)。
[Abstract]:Aim to investigate the relationship between the expression of programmed death molecule-1 (programmed death-1), programmed death molecule-1 (programmed death ligand-1), and programmed death molecule-1 (PD-L1) protein and the mutation of K-RAS gene in lung adenocarcinoma. Methods Immunohistochemical EnVision two-step method was used to detect the expression of PD-1,PD-L1 protein, and real-time fluorescence quantitative PCR was used to detect the mutation type of K-RAS gene. Results the positive rate of PD-1,PD-L1 in lung adenocarcinoma was higher than that in benign lung tissues (P0.01). The expression of PD-1,PD-L1 protein was correlated with sex, age, smoking history and differentiation degree. There was no correlation between lymph node metastasis and TNM stage (P0.05). K-RAS gene mutation occurred in 8 cases (22.2%) of 36 lung adenocarcinoma samples, and K-RAS gene mutation was associated with gender, age, smoking history and differentiation degree of the patients, and there was no correlation between K-RAS gene mutation and sex, age, smoking history and differentiation degree of lung adenocarcinoma. There was no significant difference in lymph node metastasis and TNM stage (P0.05). Correlation analysis showed that the expression of PD-1,PD-L1 protein was not associated with K-RAS mutation (P0.05). Conclusion the expression of PD-1,PD-L1 protein in lung adenocarcinoma is significantly higher than that in benign lung tissue, but it is not related to the clinicopathological features of lung adenocarcinoma and the mutation of K-RAS gene.
【作者單位】： 河北省唐山市華北理工大學附屬醫(yī)院病理科;
【基金】：2016年河北省政府資助臨床醫(yī)學優(yōu)秀人才培養(yǎng)和基礎(chǔ)課題研究(361036)
【分類號】：R734.2

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