【摘要】：【目的】研究干旱脅迫下,棉花DNA損傷修復(fù)基因的表達(dá)情況,從整體水平探討棉花DNA損傷修復(fù)相關(guān)基因表達(dá)與抗旱的相關(guān)性�！痉椒ā坑�2.5%PEG6000處理棉花幼苗,利用RNA-Seq技術(shù)對干旱脅迫下棉花幼苗的轉(zhuǎn)錄組進(jìn)行測序。【結(jié)果】從棉花干旱脅迫響應(yīng)的轉(zhuǎn)錄組中,篩選獲得差異表達(dá)的DNA損傷修復(fù)相關(guān)基因共51個,其中差異表達(dá)的上調(diào)基因23個,差異表達(dá)的下調(diào)基因28個,干旱脅迫能夠影響棉花DNA損傷修復(fù)相關(guān)基因的表達(dá)。選取4個差異基因進(jìn)行生物信息學(xué)分析及qRT-PCR驗證,HMGB1、rec A1、UDGs和GMP synthase基因的表達(dá)變幅有一定的差異,但基因的表達(dá)趨勢一致,棉花轉(zhuǎn)錄組測序結(jié)果通過qRTPCR驗證是可靠的�！窘Y(jié)論】DNA損傷修復(fù)相關(guān)基因可能與干旱脅迫有一定的相關(guān)性,干旱脅迫能夠影響棉花DNA損傷修復(fù)相關(guān)基因的表達(dá)。
[Abstract]:[objective] to study the expression of DNA damage repair genes in cotton under drought stress, and to explore the correlation between the expression of DNA damage repair genes and drought resistance in cotton seedlings. [methods] Cotton seedlings were treated with 2.5%PEG6000. The transcriptome of cotton seedlings under drought stress was sequenced by RNA-Seq technique. [results] 51 differentially expressed genes related to DNA damage repair were screened from the transcriptional groups of cotton drought stress response. Among them, 23 genes were up-regulated and 28 genes were down-regulated. Drought stress could affect the expression of genes related to DNA damage and repair in cotton. Four differentially expressed genes were selected for bioinformatics analysis and qRT-PCR verification. There were some differences in the expression of HMGB1,rec A1UDGs and GMP synthase genes, but the trend of gene expression was the same. [conclusion] DNA damage repair related genes may be related to drought stress, drought stress can affect the expression of cotton DNA damage repair related genes.
【作者單位】： 新疆大學(xué)生命科學(xué)與技術(shù)學(xué)院/新疆生物資源基因工程重點實驗室;
【基金】：國家自然科學(xué)基金-新疆聯(lián)合基金重點項目“棉花水分高效利用的關(guān)鍵基因的挖掘及抗旱種質(zhì)材料的創(chuàng)制”(U1303282)~~
【分類號】：S562

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