【摘要】：綠原酸是金銀花(Lonicera japonica Thunb.)入藥的主要化學(xué)成分,如何穩(wěn)定和提高綠原酸的含量是近年來金銀花研究的熱點(diǎn)。本研究通過對金銀花分別根施Fe、B和Mo 3種微量元素,比較處理前、后金銀花中微量元素與綠原酸含量的變化,并定量分析3種微量元素對金銀花綠原酸合成關(guān)鍵酶基因LjHCT和LjC3H1表達(dá)的影響。研究結(jié)果顯示,高濃度的Fe處理對LjHCT基因的表達(dá)有明顯的抑制作用,而中、低濃度的Fe處理可以促進(jìn)LjHCT基因的表達(dá);隨著B和Mo元素濃度的提高,LjHCT基因的表達(dá)量也逐漸增加。低濃度的Fe處理可以促進(jìn)LjC3H1基因的表達(dá),而高濃度的Fe處理對LjC3H1基因的表達(dá)具有抑制作用;B元素對LjC3H1基因表達(dá)無顯著影響,而高濃度的Mo處理可以促進(jìn)LjC3H1基因的表達(dá)。根施中、低濃度的Fe元素,中、高濃度的Mo和B元素后金銀花綠原酸含量顯著增加;而根施高濃度的Fe元素后金銀花中綠原酸含量顯著減少。研究結(jié)果表明微量元素Fe、B和Mo可通過調(diào)節(jié)綠原酸生物合成關(guān)鍵酶基因的表達(dá)從而有效促進(jìn)綠原酸的形成和積累。本研究為人工定向調(diào)控金銀花綠原酸含量、開發(fā)人工栽培金銀花專用微量元素肥料提供了理論依據(jù)。
[Abstract]:Lv Yuan Acid is (Lonicera japonica Thunb.) of Flos Lonicerae The main chemical constituents of Chinese medicine, how to stabilize and improve the content of Lv Yuan acid are the hot spot of honeysuckle research in recent years. In this study, Fe,B and Mo were applied to the root of honeysuckle, and the contents of trace elements and Lv Yuan in honeysuckle were compared before and after treatment. The effects of three trace elements on the expression of LjHCT and LjC3H1 genes of Lv Yuan acid biosynthesis in Flos Lonicerae were analyzed quantitatively. The results showed that the high concentration of Fe could inhibit the expression of LjHCT gene, while the low concentration of Fe could promote the expression of LjHCT gene, and the expression of LjHCT gene increased with the increase of the concentration of B and Mo elements. Low concentration of Fe treatment could promote the expression of LjC3H1 gene, but high concentration of Fe treatment could inhibit the expression of LjC3H1 gene, B element had no significant effect on LjC3H1 gene expression, but high concentration of Mo treatment could promote the expression of LjC3H1 gene. In root application, the content of Lv Yuan in honeysuckle increased significantly with low concentration of Mo and B element, but decreased with root application of high concentration of Fe element. The results showed that trace element Fe,B and Mo could effectively promote the formation and accumulation of Lv Yuan acid by regulating the expression of the key enzyme genes of Lv Yuan biosynthesis. This study provides a theoretical basis for the direct regulation of Lv Yuan acid content in honeysuckle flower and the development of special trace element fertilizer for artificial cultivation of honeysuckle.
【作者單位】： 凱里學(xué)院化學(xué)與材料工程學(xué)院;懷化學(xué)院生物與食品工程學(xué)院;
【基金】：貴州省科技計劃廳聯(lián)合基金項目(黔科合LH字[2014]7219) 貴州省科學(xué)技術(shù)基金項目(黔科合J字[2015]2131) 貴州省教育廳重點(diǎn)項目(黔教合KY字[2014]281) 湖南省科技計劃重點(diǎn)項目(2013FJ4324)~~
【分類號】：Q943.2;S567.79

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