【摘要】：【目的】通過分離海分枝桿菌野生株和mkl突變株的全菌蛋白,并進行差異蛋白質(zhì)組分析,以期為探索分枝桿菌重要毒力基因mkl的功能提供新思路�！痉椒ā恳院７种U菌野生株和mkl突變株為研究材料,提取全菌蛋白,i TRAQ試劑標記后進行質(zhì)譜鑒定和定量分析,并利用Uni Prot數(shù)據(jù)庫對差異蛋白進行生物信息學分析�！窘Y(jié)果】共鑒定出在野生株和mkl突變株中差異表達蛋白566個,其中在突變株中上調(diào)表達蛋白232個(比值≥1.4),下調(diào)表達蛋白334個(比值≤0.7)。生物信息學預測這些蛋白主要參與細菌脂質(zhì)代謝、細胞壁和細胞進程、中間代謝、呼吸作用等生物學功能。其中Des A3下調(diào)最顯著,其功能為脂肪酸去飽和酶,與油酸合成相關(guān),進一步驗證發(fā)現(xiàn)mkl突變株在不含油酸的固體培養(yǎng)基中生長受限,提示mkl可能在油酸的生物合成通路中發(fā)揮功能。【結(jié)論】通過i TRAQ分析了海分枝桿菌mkl突變株和野生株的差異表達蛋白譜,發(fā)現(xiàn)可能影響分枝桿菌油酸、脂質(zhì)等合成代謝通路,為進一步研究mkl基因在分枝桿菌致病中發(fā)揮作用的相關(guān)機制奠定了基礎。
[Abstract]:[objective] to isolate the whole bacterial proteins from wild and mkl mutants of Mycobacterium sea, and to analyze the differential proteome. In order to provide a new idea for exploring the function of the important virulence gene mkl of Mycobacterium sp. [methods] the wild and mkl mutants of Mycobacterium martensii were extracted and labeled with, i TRAQ reagent for mass spectrometry and quantitative analysis. A total of 566 differentially expressed proteins were identified in wild and mkl mutants, among which 232 proteins were upregulated in mutants (ratio 鈮，

本文編號：2406026