【摘要】：選取黃河鯉新品系親本和子代共450尾,5對微衛(wèi)星引物和4個基因區(qū)段擴(kuò)增引物開展基因型檢測,并檢測它們與體重的關(guān)聯(lián)性以及分析相應(yīng)的遺傳效應(yīng)。結(jié)果顯示:Koi42和4個SNPs位點(diǎn)對體重有顯著的影響,獲得具有超過富集2個優(yōu)勢基因型的候選親本共13尾,富集優(yōu)勢分子標(biāo)記基因型的候選親本生長性能優(yōu)勢明顯。通過多元逐步回歸分析,利用AIC最佳模型篩選到COⅠ626、D-Loop253和Koi42共3個位點(diǎn),發(fā)現(xiàn)Koi42貢獻(xiàn)率較大,經(jīng)Fisher精確性檢驗(yàn),發(fā)現(xiàn)其與性別存在關(guān)聯(lián),其加性效應(yīng)接近顯著水平(P0.05)。檢測的多個上位效應(yīng)組分中,僅有D-Loop253和Koi42的加性效應(yīng)間的互作達(dá)到顯著水平。對遺傳方差組分進(jìn)行剖分發(fā)現(xiàn),加性方差占11.4%,兩個位點(diǎn)的加性效應(yīng)構(gòu)成的上位效應(yīng)占到77.5%,因此這3個分子標(biāo)記對體重的影響主要以上位效應(yīng)為主,而且是兩兩加性效應(yīng)的占比較大,可以推斷出D-Loop 253和Koi42兩個分子標(biāo)記的上位效應(yīng)起主要作用。綜上所述結(jié)果提示Koi42及與其有互作的D-Loop253可用于黃河鯉新品系的標(biāo)記輔助選擇,可以開展多個分子標(biāo)記的富集選擇。
[Abstract]:A total of 450 parents and progenies of Yellow River carp were selected. Five pairs of microsatellite primers and four amplified primers were used to detect their genotypes, and their association with body weight and genetic effects were analyzed. The results showed that Koi42 and 4 SNPs loci had a significant effect on body weight, and 13 candidate parents with more than 2 dominant genotypes were obtained. By multivariate stepwise regression analysis, CO 鈪，

本文編號：2403476