【摘要】：為闡明泡桐多倍體的耐鹽分子機(jī)制,以南方泡桐二倍體及對(duì)應(yīng)的四倍體為試驗(yàn)材料,以白花泡桐基因組為參考序列,利用RNA-Seq測(cè)序技術(shù),研究了鹽處理前后南方泡桐四倍體和對(duì)應(yīng)二倍體的基因表達(dá)變化。通過比對(duì)分析,共鑒定出與鹽脅迫相關(guān)的差異表達(dá)基因2 940個(gè)。對(duì)這些差異基因進(jìn)行GO功能分類,結(jié)果顯示差異基因共參與了39個(gè)分類,集中在細(xì)胞、結(jié)合、催化等分類功能區(qū)的數(shù)量最多。KEGG代謝通路分析發(fā)現(xiàn),這些差異表達(dá)基因主要參與了Plant hormone signal transduction,Carbon metabolism,Biosynthesis of amino acid等,其中編碼MYB、WRKY、bZIP、ATPase等的基因差異表達(dá)顯著。表明這些基因可能是潛在的鹽脅迫響應(yīng)基因。采用qRTPCR技術(shù)驗(yàn)證了隨機(jī)挑選的9個(gè)差異表達(dá)基因,結(jié)果與轉(zhuǎn)錄組測(cè)序數(shù)據(jù)趨勢(shì)一致。
[Abstract]:In order to elucidate the molecular mechanism of salt tolerance of Paulownia paulownia polyploid, the diploid and tetraploid of Paulownia paulownia were used as experimental materials, the genome of Paulownia paulownia was used as reference sequence, and the RNA-Seq sequencing technique was used. The changes of gene expression of paulownia tetraploid and diploid were studied before and after salt treatment. A total of 2940 differentially expressed genes related to salt stress were identified by comparative analysis. The GO functional classification of these differentially expressed genes showed that the differentially expressed genes were involved in 39 classifications, mainly in cell, binding, catalytic and other functional regions. KEGG metabolic pathway analysis showed that, These differentially expressed genes were mainly involved in Plant hormone signal transduction,Carbon metabolism,Biosynthesis of amino acid et al. Among them, the differentially expressed genes encoded MYB,WRKY,bZIP,ATPase et al. These genes may be potential salt stress response genes. Nine differentially expressed genes were randomly selected by qRTPCR. The results were consistent with the trend of transcriptome sequencing data.
【作者單位】： 河南農(nóng)業(yè)大學(xué)泡桐研究所;
【基金】：國(guó)家公益性林業(yè)行業(yè)科研專項(xiàng)(201004002)
【分類號(hào)】：S792.43

【相似文獻(xiàn)】

相關(guān)期刊論文 前6條

1 王鵬偉;趙改麗;翟曉巧;范國(guó)強(qiáng);;南方泡桐突變體構(gòu)建及黃化苗體外植株再生的研究[J];河南農(nóng)業(yè)大學(xué)學(xué)報(bào);2010年04期

2 王楊;張曉申;鄧敏捷;趙振利;范國(guó)強(qiáng);;南方泡桐二倍體及其四倍體的光合特性研究[J];江西農(nóng)業(yè)學(xué)報(bào);2013年08期

3 范國(guó)強(qiáng);魏真真;楊志清;;南方泡桐同源四倍體的誘導(dǎo)及其體外植株再生[J];西北農(nóng)林科技大學(xué)學(xué)報(bào)(自然科學(xué)版);2009年10期

4 鄧敏捷;張曉申;范國(guó)強(qiáng);趙振利;董焱鵬;魏振;;四倍體南方泡桐和四倍體白花泡桐對(duì)鹽脅迫的生理響應(yīng)研究[J];河南農(nóng)業(yè)大學(xué)學(xué)報(bào);2013年06期

5 張曉申;范國(guó)強(qiáng);鄧敏捷;董焱鵬;趙振利;;南方泡桐二倍體及其同源四倍體AFLP和MSAP的差異[J];東北林業(yè)大學(xué)學(xué)報(bào);2014年02期

6 歐陽名玲,熊耀國(guó),陶棟偉,金烈誼,陳玉波,李榮幸,劉廷志;南方桐區(qū)泡桐雜交育種試驗(yàn)[J];浙江林業(yè)科技;1979年04期

相關(guān)碩士學(xué)位論文 前1條

1 魏真真;豫雜一號(hào)泡桐和南方泡桐同源四倍體誘導(dǎo)及其體外植株再生的研究[D];河南農(nóng)業(yè)大學(xué);2008年

，

本文編號(hào)：2397454