【摘要】：采用RT-PCR方法,首次從木薯華南8號(SC8)克隆一個環(huán)指蛋白RFP基因家族成員MeRFP8.該基因c DNA包含1059 bp的開放閱讀框,編碼一個由352個氨基酸殘基組成的蛋白質(zhì).該蛋白質(zhì)分子質(zhì)量為39.23 ku,理論等電點為4.84,C末端含有保守的結(jié)構(gòu)域RING finger domain(Ring-H2 zinc finger),屬于C3H2C3型環(huán)指蛋白.利用實時熒光定量PCR分析MeRFP8基因在木薯SC8及其同源四倍體葉片的表達水平,結(jié)果顯示:5℃低溫脅迫24 h內(nèi),SC8和其四倍體MeRFP8基因先下調(diào)表達,后上調(diào)表達,呈"V"字型.而且MeRFP8基因在SC8的表達變化水平比其同源四倍體大.推測MeRFP8基因可能參與木薯的低溫響應(yīng).
[Abstract]:MeRFP8., a member of RFP gene family of ring finger protein, was cloned for the first time from cassava South China 8 (SC8) by RT-PCR method. The c DNA gene contains an open reading frame of 1059 bp and encodes a protein consisting of 352 amino acid residues. The molecular weight of the protein is 39.23 ku, and the theoretical isoelectric point is 4.84%. The conserved domain RING finger domain (Ring-H2 zinc finger), belongs to the C3H2C3 ring finger protein. The expression of MeRFP8 gene in cassava SC8 and its autotetraploid leaves was analyzed by real-time fluorescence quantitative PCR. The results showed that the expression of SC8 and its tetraploid MeRFP8 gene was down-regulated at 5 鈩，

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