【摘要】：脫落酸不敏感基因(ABA-insensitive,ABI)是調(diào)控植物逆境脅迫應(yīng)答、種子脫落和休眠等的一類轉(zhuǎn)錄因子。生物信息學(xué)分析發(fā)現(xiàn),普通蕎麥ABI(CL38501)基因c DNA全長為963 bp,編碼320個氨基酸,含有典型的b ZIP結(jié)構(gòu)域和ABI5家族保守區(qū)Ⅱ,推測該基因可能編碼ABI5家族蛋白。與野生種質(zhì)相比,花蕾期和開花期栽培品種ABA信號轉(zhuǎn)導(dǎo)途徑中絕大多數(shù)基因表達量上調(diào),其中,ABI基因表達量分別上調(diào)4.42和3.66倍。對9個栽培品種和5份野生種質(zhì)的ABI基因進行PCR擴增,發(fā)現(xiàn)14份材料的405個位點中多態(tài)位點為30個,9個栽培品種間僅含1個多態(tài)位點,5個野生種質(zhì)間含有7個多態(tài)位點,說明普通蕎麥ABI基因序列高度保守。栽培品種與野生種質(zhì)ABI序列存在11個共同氨基酸差異位點,蛋白構(gòu)象預(yù)測發(fā)現(xiàn)第32位點和第60位點氨基酸發(fā)生置換導(dǎo)致蛋白構(gòu)象發(fā)生變化,表明其可能與栽培馴化過程中蕎麥喪失休眠與落粒特性相關(guān)。
[Abstract]:Abscisic acid insensitive gene (ABA-insensitive,ABI) is a kind of transcription factor that regulates plant stress response, seed abscission and dormancy. Bioinformatics analysis showed that the total length of c DNA of ABI (CL38501) gene in buckwheat was 963 bp, encoding 320 amino acids, which contained the typical b ZIP domain and ABI5 family conserved region 鈪，

本文編號：2342296