發(fā)布時(shí)間：2018-10-11 10:59

【摘要】：為了探尋歐洲白樺變種裂葉樺的葉緣分裂機(jī)制。以2種樺樹為研究對(duì)象,在葉片形態(tài)特征和解剖特征觀測(cè)的基礎(chǔ)上,進(jìn)行了頂芽及葉片葉尖、裂片及凹缺等不同組織部位相關(guān)基因表達(dá)定量分析、內(nèi)源激素IAA含量測(cè)定以及IAA活體標(biāo)記的GUS染色分析。結(jié)果表明,裂葉樺葉片兩側(cè)葉緣自葉基到葉尖分裂出由大到小3~4個(gè)裂片,葉背面的葉脈呈明顯的管狀隆起,葉寬、平均二級(jí)脈間距、主脈橫切面積等分別較歐洲白樺大了21.12%、12.64%、21.46%。BpmiR164s及靶基因的組織表達(dá)特性分析結(jié)果證明,BpmiR164-2及靶基因BpCUC2與裂片的形成關(guān)系密切,是裂葉性狀產(chǎn)生的主要調(diào)控基因。生長(zhǎng)素輸出載體BpPINs基因的組織表達(dá)特性分析結(jié)果證明,6個(gè)基因中只有BpPIN2和BpPIN5在裂葉樺的葉尖、裂片及頂芽等組織器官中呈現(xiàn)上調(diào)表達(dá)。內(nèi)源激素IAA的活體標(biāo)記發(fā)現(xiàn),在頂芽、幼葉中IAA的含量較高,而在葉片中,葉尖、葉齒及裂片處是激素的高表達(dá)部位,利用液相色譜—質(zhì)譜聯(lián)用儀(LC-MS)對(duì)2種樺樹的上述不同組織部位的生長(zhǎng)素測(cè)定也證明了激素的上述分布特點(diǎn)。綜合上述,裂葉樺裂片產(chǎn)生是激素在葉脈、葉緣的極性運(yùn)輸及相關(guān)基因調(diào)控的結(jié)果。
[Abstract]:In order to find out the mechanism of leaf margin division of Betula platyphylla var. Bai Hua in Europe. Based on the observation of morphological and anatomical characteristics of two species of birch, quantitative analysis of gene expression in different tissues such as apical bud, leaf tip, lobes and concave was carried out. The content of endogenous hormone IAA and GUS staining of IAA in vivo were determined. The results showed that the leaves of Betula lobata were separated from the base to the tip, and the veins on the back of the leaf were obviously tubular, with the width of the leaf, and the average secondary vein spacing. The cross sectional area of main veins was 21.12 / 12.64 and 21.46. BpmiR164s and target gene were respectively larger than those of Bai Hua in Europe. The results showed that BpmiR164-2 and target gene BpCUC2 were closely related to the formation of lobes and were the main regulatory genes for the formation of lobes. The results of tissue expression analysis of BpPINs gene of auxin export vector showed that only BpPIN2 and BpPIN5 showed upregulated expression in tissues and organs of Betula lobata such as tip, lobes and terminal buds of Betula lobata. In vivo marker of endogenous hormone IAA, it was found that the content of IAA was higher in terminal bud and young leaf, while in leaf, tip, tooth and lobes were the high expression sites of hormone. The determination of auxin in different tissues of two birch species by liquid chromatography-mass spectrometry (LC-MS) also proved the distribution of hormones. To sum up above, the production of Betula fissure lobes is the result of the polar transport of hormones in leaf veins and margins and the regulation of related genes.
【作者單位】： 東北林業(yè)大學(xué)林木遺傳育種國(guó)家重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(31370660)
【分類號(hào)】：S792.15

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