發(fā)布時(shí)間：2018-09-18 19:24

【摘要】：赤霉素(GAs)對植物生長發(fā)育有重要影響,赤霉素氧化酶基因(GA2ox)在GAs合成過程中起重要調(diào)控作用。本文從不同芹菜(Apium graveolens)品種‘六合黃心芹’和‘文圖拉’中分別克隆得到編碼赤霉素氧化酶的基因AgGA2ox,序列分析顯示兩個(gè)品種AgGA2ox基因均包含一個(gè)全長996 bp的開放閱讀框,編碼331個(gè)氨基酸,在核苷酸水平上有8個(gè)堿基位點(diǎn)的差異,氨基酸水平上有2個(gè)位點(diǎn)的差異。AgGA2ox蛋白在‘六合黃心芹’和‘文圖拉’兩個(gè)品種中的相對分子質(zhì)量分別為37 044.6和36984.5,理論等電點(diǎn)均為8.31。進(jìn)化分析表明,芹菜Ag GA2ox在植物進(jìn)化間具有高度保守性,與西洋參(Panax quinquefolius)進(jìn)化關(guān)系最為接近。實(shí)時(shí)定量PCR顯示,AgGA2ox基因表達(dá)具有組織特異性,在芹菜葉柄中表達(dá)量最高;另外,AgGA2ox基因在4種非生物逆境條件[4°C低溫、38°C高溫、20%聚乙二醇(polyethylene glycol,PEG)及0.2 mol·L~(-1)NaCl]下均有響應(yīng),低溫處理下AgGA2ox基因表達(dá)為明顯上調(diào),高溫、干旱和鹽處理下AgGA2ox基因表達(dá)為下調(diào)。‘六合黃心芹’與‘文圖拉’AgGA2ox基因在逆境下的表達(dá)差異可能與不同品種抗逆性有關(guān)。
[Abstract]:Gibberellin (GAs) plays an important role in plant growth and development, and the GA2ox gene plays an important role in the regulation of GAs synthesis. In this paper, two genes encoding GA2ox were cloned from different celery cultivars, Apium graveolens and Ventura, respectively. The AgGA2ox gene contained an open reading frame of 996 bp, encoding 331 amino acids. There were 8 nucleotide loci and 2 amino acid loci at the nucleotide level. The relative molecular weight of AgGA2ox protein in'Liuhe Huangxinqin'and'Ventura' were 37 044.6 and 36 984.5, respectively. Evolutionary analysis showed that AgGA2ox gene was highly conserved in plant evolution and was most closely related to Panax quinquefolius. Real-time quantitative PCR showed that AgGA2ox gene was tissue-specific and expressed most in celery petiole. In addition, AgGA2ox gene was expressed in four abiotic stress conditions. [4 degree C low temperature, 38 degree C high temperature, 20% polyethylene glycol (PEG) and 0.2 mol (- 1) NaCl] all responded. The expression of AgGA2ox gene was significantly up-regulated under low temperature treatment, but down-regulated under high temperature, drought and salt treatment. Different varieties are resistant to stress.
【作者單位】： 南京農(nóng)業(yè)大學(xué)作物遺傳與種質(zhì)創(chuàng)新國家重點(diǎn)實(shí)驗(yàn)室園藝學(xué)院農(nóng)業(yè)部華東地區(qū)園藝作物生物學(xué)與種質(zhì)創(chuàng)新重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金(31272175) 教育部新世紀(jì)優(yōu)秀人才支持計(jì)劃項(xiàng)目(NCET-11-0670) 江蘇省自然科學(xué)基金杰出青年基金(BK20130027) 江蘇高校優(yōu)勢學(xué)科建設(shè)項(xiàng)目(PAPD)~~
【分類號(hào)】：S636.3

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