【摘要】：根據(jù)蜻蜓鳳梨轉(zhuǎn)錄組信息,通過RACE技術(shù)(rapid amplification of cDNA ends,RACE)獲得蜻蜓鳳梨中一個DELLA蛋白的cDNA序列,并將其命名為AfGAI。AfGAI cDNA全長1 960 bp,開放閱讀框為1 764 bp,編碼587個氨基酸。氨基酸序列分析結(jié)果顯示,AfGAI蛋白含有DELLA蛋白家族共有的功能結(jié)構(gòu)域:DELLA結(jié)構(gòu)域和GRAS結(jié)構(gòu)域,與菠蘿(Ananas comosus)、海棗(Phoenix dactylifera)、油棕(Elaeis guineensis)、巨桉(Eucalyptus grandis)、陸地棉(Gossypium hirsutum)中的GAI同源性分別為90%、73%、73%、65%、59%。進(jìn)一步構(gòu)建了35S:AfGAI過表達(dá)載體,為解析鳳梨科植物開花機(jī)理提供理論依據(jù)。
[Abstract]:According to the transcriptome information of dragonfly and pineapple, the cDNA sequence of a DELLA protein in dragonfly and pineapple was obtained by rapid amplification of cDNA ends (RACE). The full length of the DELLA protein was 1 960 bp, the open reading frame was 1 764 BP and 587 amino acids were encoded. The amino acid sequence analysis showed that the AFGAI protein contained DELLA eggs. The common functional domains of the Bai family are DELLA and GRAS domains, which are 90%, 73%, 73%, 65% and 59% homologous to GAI in pineapple (Ananas comosus), jujube (Phoenix dactylifera), oil palm (Elaeis guineensis), Eucalyptus grandis, and upland cotton (Gossypium hirsutum). The 35S: AfGAI overexpression vector was constructed to understand the above-mentioned genes. To provide theoretical basis for flowering mechanism of pineapple plants.
【作者單位】： 海南大學(xué)農(nóng)學(xué)院;中國熱帶農(nóng)業(yè)科學(xué)院熱帶作物品種資源研究所農(nóng)業(yè)部華南熱帶作物基因資源與種質(zhì)創(chuàng)制重點(diǎn)開放實驗室海南省熱帶作物遺傳改良與創(chuàng)新重點(diǎn)實驗室;
【基金】：國家自然基金面上項目(31372106);國家自然基金青年科學(xué)基金項目(31601793) 海南省自然科學(xué)基金面上項目(20163127)共同資助
【分類號】：Q943.2;S682.3

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